Comprehensive Analysis of Rice Genes Using the FOX-Hunting System

DOI

Bibliographic Information

Other Title
  • FOX Hunting Systemを用いたイネ遺伝子の網羅的機能解析
Published
2008
DOI
  • 10.14841/jspp.2008.0.1003.0
Publisher
The Japanese Society of Plant Physiologists

Description

For systematic and genome-wide functional analysis of rice genes, we are taking advantage of the Full-length cDNA Over-eXpresser (FOX) gene-hunting system. We collected the plasmid DNAs of 13,980 independent clones from RIKEN to produce a FOX library by placing individual cDNA under the control of ZmUbi-1 promoter. Then the FOX library was transformed into rice via Agrobacterium. So far, we have generated approximately 12,000 RIKEN-FOX-rice lines. Sequencing analysis of the PCR fragments carrying FL-cDNAs from 8,615 FOX-rice lines identified FL-cDNAs in 8,225 lines, and a database search classified the cDNAs into 5,462 independent ones. Approximately 16.6% of FOX-rice lines examined showed altered growth or morphological characteristics. We also have constructed rice FOX libraries containing 13,823 independent FL-cDNAs from the Foundation for Advancement of International Science (FAIS), and are generating FAIS-FOX-rice lines. Our collection of the FOX-rice lines should be a valuable tool to efficiently identify agronomically important genes.

Journal

Details 詳細情報について

  • CRID
    1390001205630840192
  • NII Article ID
    130006991753
  • DOI
    10.14841/jspp.2008.0.1003.0
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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