{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1390001205783498240.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.20632/vso.75.1_1"}},{"identifier":{"@type":"NDL_BIB_ID","@value":"5648798"}},{"identifier":{"@type":"URI","@value":"http://id.ndl.go.jp/bib/5648798"}},{"identifier":{"@type":"URI","@value":"https://ndlsearch.ndl.go.jp/books/R000000004-I5648798"}},{"identifier":{"@type":"NAID","@value":"110002883428"}},{"identifier":{"@type":"URI","@value":"https://search.jamas.or.jp/link/ui/2001143947"}}],"dc:title":[{"@language":"ja","@value":"1. α-トコフェロールおよびカロテノイドのリン脂質膜における存在様態と一重項酸素不活化反応のキネティクス"},{"@language":"en","@value":"Kinetics and Dynamics of α-Tocopherol and Carotenoids for Deactivating Singlet Oxygen in Phospholipid Membranes"},{"@value":"α-トコフェロールおよびカロテノイドのリン脂質膜における存在様態と一重項酸素不活化反応のキネティクス"},{"@language":"ja-Kana","@value":"アルファ トコフェロール オヨビ カロテノイド ノ リン シシツ マク ニ オケル ソンザイ ヨウタイ ト 1ジュウコウ サンソ フカツカ ハンノウ ノ キネティクス"}],"dc:language":"ja","description":[{"type":"abstract","notation":[{"@language":"en","@value":"The singlet oxygen (^1O_2) deactivating rate constants (k_s) of α-tocopherol (α-Toc) and carotenoids such as β-carotene, astaxanthin and canthaxanthin and their inhibiting activities for ^1O_2-dependentlipid peroxidation (IC_50' that is, the concentration required for 50% inhibition of lipid peroxidation) were investigated in liposomes and compared to those in EtOH solution. Liposomes were prepared from dimyristoylphosphatidylcholine to measure the k_s and egg yolk phosphatidylcholine to measure the IC_50 values. ^1O_2 was generated by photoirradiation using three photosensitizers, Rose bengal and methylene blue, which generate ^1O_2 at the membrane surface or in bulk water, and 2-(1-pyrene) dodecanoic acid, which generates ^1O_2 in the membrane hydrophobic inner region. The k_s and IC_50 values were determined in membranes considering the following two points: (1) antioxidants exist in membranes and their active moieties are distributed in different regions of membranes at different concentrations; (2) dynamics of ^1O_2 in membranes (generation site of ^1O_2 depending on the localization of photosensitizer in membranes, and solubility of ^1O_2 which differs in the site of membranes). In conclusion, the following three factors are experimentally confirmed to be important for the consideration of ^1O_2 deactivating activities of α-Toc and carotenoids in membranes:(1) the concentration of antioxidants (EtOH solution < membrane), especially the local concentration of their active moieties in membranes (in the case of OH-group of α-Toc, 0%, 50-60%, and 40-50% at polar zone, hydrogen belt, and hydrophobic core, respectively, of the membranes); (2) the mobility of antioxidants (EtOH solution > membrane), the mobility of membrane phospholipid (liquid crystalline state > gel state), the mobility of antioxidants in membranes (antioxidants located at one half of the bilayer membrane such as α-tocopherol > antioxidants located across the bilayer such as β-carotene; antioxidants interactive with polar head group of lipid in membrane such as astaxanthin < antioxidants nointeractive with polar head group of lipid in membrane such as β-carotene), and the local mobility of their active moieties (in case of OH-group of α-Toc, membrane surface < membrane inner region); (3) the dielectric constant (micropolarity) at the domains in membranes, that is higher in membrane surface than in membrane inner region, where the active moieties of antioxidants deactivate ^1O_2 (in case of α-Toc, reactivity is high in the solution with high dielectric constant)."}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1410001205783498240","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000006798665"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0170975"}],"foaf:name":[{"@language":"ja","@value":"福澤 健治"},{"@language":"en","@value":"FUKUZAWA Kenji"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Faculty of Pharmaceutical Sciences, Tokushima University"},{"@language":"ja","@value":"徳島大学薬学部衛生化学教室"}]},{"@id":"https://cir.nii.ac.jp/crid/1410001205783498241","@type":"Researcher","foaf:name":[{"@language":"ja","@value":"徳村 彰"},{"@language":"en","@value":"TOKUMURA Akira"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Faculty of Pharmaceutical Sciences, Tokushima University"},{"@language":"ja","@value":"徳島大学薬学部衛生化学教室"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"0006386X"},{"@type":"EISSN","@value":"2424080X"},{"@type":"NDL_BIB_ID","@value":"000000020049"},{"@type":"ISSN","@value":"0006386X"},{"@type":"LISSN","@value":"0006386X"},{"@type":"NCID","@value":"AN00207833"}],"prism:publicationName":[{"@language":"ja","@value":"ビタミン"},{"@language":"en","@value":"VITAMINS"}],"dc:publisher":[{"@language":"en","@value":"THE VITAMIN SOCIETY OF JAPAN"},{"@language":"ja","@value":"公益社団法人 日本ビタミン学会"}],"prism:publicationDate":"2001","prism:volume":"75","prism:number":"1","prism:startingPage":"1","prism:endingPage":"13"},"url":[{"@id":"http://id.ndl.go.jp/bib/5648798"},{"@id":"https://ndlsearch.ndl.go.jp/books/R000000004-I5648798"},{"@id":"https://search.jamas.or.jp/link/ui/2001143947"}],"availableAt":"2001","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=%CE%B1-tocopherol","dc:title":"α-tocopherol"},{"@id":"https://cir.nii.ac.jp/all?q=carotenoid","dc:title":"carotenoid"},{"@id":"https://cir.nii.ac.jp/all?q=singlet%20oxygen","dc:title":"singlet oxygen"},{"@id":"https://cir.nii.ac.jp/all?q=lipid%20peroxidation","dc:title":"lipid peroxidation"},{"@id":"https://cir.nii.ac.jp/all?q=phospholipid%20membranes","dc:title":"phospholipid membranes"},{"@id":"https://cir.nii.ac.jp/all?q=liposomes","dc:title":"liposomes"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1361137045028929536","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@value":"Solvent effects in dye-sensitized photooxidation reactions"}]},{"@id":"https://cir.nii.ac.jp/crid/1361418521355844480","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@value":"Determination of rate constants of reaction and lifetimes of singlet oxygen in solution by a flash photolysis technique"}]},{"@id":"https://cir.nii.ac.jp/crid/1361699993743675392","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@value":"Radiationless decay of singlet molecular oxygen in solution. Experimental and theoretical study of electronic-to-vibrational energy transfer"}]},{"@id":"https://cir.nii.ac.jp/crid/1362262945987296000","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@value":"Location and dynamics of α-tocopherol in model phospholipid membranes with different charges"}]},{"@id":"https://cir.nii.ac.jp/crid/1363388844543308288","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@value":"Kinetics and Dynamics of Singlet Oxygen Scavenging by α-Tocopherol in Phospholipid Model Membranes"}]},{"@id":"https://cir.nii.ac.jp/crid/1363388844674615552","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@value":"Rate constants for quenching singlet oxygen and activities for inhibiting lipid peroxidation of carotenoids and α‐tocopherol in liposomes"}]},{"@id":"https://cir.nii.ac.jp/crid/1363670319236466176","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@value":"Antioxidant activity of β‐carotene‐related carotenoids in solution"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001205701349632","@type":"Article","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@language":"ja","@value":"卵黄レシチンリポソーム膜におけるビタミンEのラジカル消去反応速度(研究論文紹介)"},{"@language":"en","@value":"Kinetic study of aroxyl radical-scavenging action of vitamin E in membranes of egg yolk phosphatidylcholine vesicles"},{"@value":"卵黄レシチンリポソーム膜におけるビタミンEのラジカル消去反応速度"},{"@language":"ja-Kana","@value":"ランオウ レシチンリポソームマク ニ オケル ビタミン E ノ ラジカル ショウキョ ハンノウ ソクド"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001205760479232","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"ja","@value":"(3)膜におけるビタミンEの動態と抗酸化機能 (<シリーズ>ビタミン研究のブレークスルー : 「ビタミンE」)"},{"@value":"膜におけるビタミンEの動態と抗酸化機能"},{"@language":"ja-Kana","@value":"マク ニ オケル ビタミン E ノ ドウタイ ト コウサンカ キノウ"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282680677555840","@type":"Article","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@language":"ja","@value":"膜におけるビタミンEの抗酸化ダイナミクス"},{"@language":"en","@value":"Antioxidant Dynamics of α-Tocopherol in Membranes"},{"@language":"ja-Kana","@value":"マク ニ オケル ビタミン E ノ コウサンカ ダイナミクス"}]},{"@id":"https://cir.nii.ac.jp/crid/1570009751741748992","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Lycopene as the most efficient biological carotenoid singlet oxygen quencher"}]},{"@id":"https://cir.nii.ac.jp/crid/1570291224633250944","@type":"Article","relationType":["cites"]},{"@id":"https://cir.nii.ac.jp/crid/1570572699609960448","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Location, antioxidant and recycling dynamics of α-tocopherol in liposome membranes."}]},{"@id":"https://cir.nii.ac.jp/crid/1570572699609961600","@type":"Article","relationType":["cites"]},{"@id":"https://cir.nii.ac.jp/crid/1570572700859618560","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Zeaxanthin (dihydroxy-βcarotene) but not β-carotene rigidifies lipid membranes : a ^1HNMR study of carotenoid-egg phosphatidylcholine liposomes"}]},{"@id":"https://cir.nii.ac.jp/crid/1571417126437575296","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Polarity studies on the head group of single-layered phosphatidylcholine-α-tocopherol vesicles."}]},{"@id":"https://cir.nii.ac.jp/crid/1571417126626077824","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Fluorescence study of the location and dynamics of α-tocopherol in phospholipid vesicles"}]},{"@id":"https://cir.nii.ac.jp/crid/1571698599516803328","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Singlet oxygen scavenging in phospholipid membranes."}]},{"@id":"https://cir.nii.ac.jp/crid/1571698601412956032","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Model of singlet oxygen scavenging by α-tocopherol in biomembranes"}]},{"@id":"https://cir.nii.ac.jp/crid/1571980074493512576","@type":"Article","relationType":["cites"]},{"@id":"https://cir.nii.ac.jp/crid/1572543026519861248","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Comparison of the effects of inserted C40- and C50-terminally dihydroxylaed carotenoids on the mechanical properities of various phospholipid vesicles."}]},{"@id":"https://cir.nii.ac.jp/crid/1572543026554070400","@type":"Article","relationType":["cites"]},{"@id":"https://cir.nii.ac.jp/crid/1572824499423645568","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Singlet oxygen scavenging by α-tocopherol and β-carotene: kinetic studies in phospholipid membranes and ethanol solution."}]},{"@id":"https://cir.nii.ac.jp/crid/1573668924353778432","@type":"Article","relationType":["cites"]},{"@id":"https://cir.nii.ac.jp/crid/1573950401230924416","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Products formed by photosensitized oxidation of unsaturated fatty acid esters."}]},{"@id":"https://cir.nii.ac.jp/crid/1574231874307199104","@type":"Article","relationType":["cites"]},{"@id":"https://cir.nii.ac.jp/crid/1574231876390899968","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Scavenging of radicals by vitamin E in the membranes as studied by spin labeling"}]}],"dataSourceIdentifier":[{"@type":"JALC","@value":"oai:japanlinkcenter.org:2003535175"},{"@type":"NDL_SEARCH","@value":"oai:ndlsearch.ndl.go.jp:R000000004-I5648798"},{"@type":"CIA","@value":"110002883428"}]}