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- OKANIWA Koji
- Graduate School of Medical Sciences, Kitasato University
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- WADA Takaaki
- Mitsuwadai General Hospital
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- UJIHIRA Masanobu
- School of Allied Health Sciences, Kitasato University
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- ICHIKAWA Hinako
- School of Allied Health Sciences, Kitasato University
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- MATSUMURA Yumi
- Shirohigebashi Hospital
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- MABUCHI Kiyoshi
- School of Allied Health Sciences, Kitasato University
Bibliographic Information
- Other Title
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- 1417 人工組織のガラス状態における有効温度伝導率の測定
Description
The purpose of this study is to assess the vitrified artificial tissue with the effective thermal diffusivity. Human fibroblast cultured into collagen sponge was used for the test artificial tissue, frozen at a cooling rate of 30∿1200℃/min from room temperature to liquid-nitrogen temperature (-196℃). The test chamber (Φ24×4mm) for freezing the test artificial tissue was in contact with a carbon heater that was on a copper block, which was immersed in liquid-nitrogen. The temperature of the test artificial tissue was increased stepwise from -190℃ to -185℃, and then the effective thermal diffusivity was deter-mined by Laplace transform method. After the measurement of the effective thermal diffusivety, post-thaw viability of human fibroblast was measured by trypan blue exclusion assay. As a result, the effective thermal diffusivity decresed and the viability increased with increasing the cooling rate. The decrease of the effective thermal diffusivity and the increase of the viability show the decrease of quantity of intra-and extracellular ice formation in the artificial tissue. Our results demonstrate that the vitrified artificial tissue can be assessed with the effective thermal diffusivity.
Journal
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- The proceedings of the JSME annual meeting
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The proceedings of the JSME annual meeting 2000.2 (0), 259-260, 2000
The Japan Society of Mechanical Engineers
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Details 詳細情報について
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- CRID
- 1390001206059194368
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- NII Article ID
- 110002522729
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- ISSN
- 24331325
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- Text Lang
- ja
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed