Construction of DNA-Au Nanoparticles Multilayer and Its Application to Detection of DNA Hybridization

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  • Liu Hongchun
    Department of Ecosystem Engineering, Graduate School of Engineering, The University of Tokushima Core Research for Evolution Science & Technology (CREST), Japan Science & Technology Corporation (JST)
  • Yamaguchi Akira
    Department of Chemistry, Graduate School of Science, The University of Tohoku
  • Hayashida Masayuki
    Core Research for Evolution Science & Technology (CREST), Japan Science & Technology Corporation (JST) Research Institute for Electronic Science, Hokkaido University
  • Matsuo Shigeki
    Department of Ecosystem Engineering, Graduate School of Engineering, The University of Tokushima Core Research for Evolution Science & Technology (CREST), Japan Science & Technology Corporation (JST)
  • Misawa Hiroaki
    Core Research for Evolution Science & Technology (CREST), Japan Science & Technology Corporation (JST) Research Institute for Electronic Science, Hokkaido University

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We have developed a sensitive detection system for DNA hybridization containing DNA-Au nanoparticles (DNA-GPs) multilayer which leads to the enhancement of the detection sensitivity of surface plasmon resonance (SPR) signals. The SPR angular shift, which is the difference in the SPR angles before and after hybridization, becomes amplified with increasing number of layers of the DNA-GPs. A similar increase in SPR angular shift was obtained for the target DNA with low concentration. The reason for the enhancement is attributed to the interaction between Au nanoparticles and the Au film, as well as the increase in immobilized probe DNA content on the substrate.

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