{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1390001206361322368.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1265/jjh.44.615"}},{"identifier":{"@type":"PMID","@value":"2554035"}},{"identifier":{"@type":"NDL_BIB_ID","@value":"3251118"}},{"identifier":{"@type":"URI","@value":"http://id.ndl.go.jp/bib/3251118"}},{"identifier":{"@type":"URI","@value":"https://ndlsearch.ndl.go.jp/books/R000000004-I3251118"}},{"identifier":{"@type":"URI","@value":"http://www.jstage.jst.go.jp/article/jjh1946/44/2/44_2_615/_pdf"}},{"identifier":{"@type":"NAID","@value":"130000999616"}},{"identifier":{"@type":"URI","@value":"https://search.jamas.or.jp/link/ui/1990154456"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@language":"en","@value":"The decomposition and aggregation of rat lens protein induced by selenite in vitro and in vivo."},{"@language":"ja","@value":"<I>In vitro</I>および<I>In vivo</I>における亜セレン酸によるラット水晶体蛋白の分解と凝集物形成について"},{"@value":"In vitroおよびIn vivoにおける亜セレン酸によるラット水晶体蛋白の分解と凝集物形成について〔英文〕"},{"@language":"ja-Kana","@value":"In vitro オヨビ In vivo ニ オケル ア セレンサン ニ ヨル"}],"dc:language":"en","description":[{"type":"abstract","notation":[{"@language":"en","@value":"One of the toxic effects of selenite is the development of bilateral nuclear cataracts in suckling rats. These lesions can be induced by a single subcutaneous injection of sodium selenite. Several studies have shown some biochemical changes associated with the selenite-induced cataract<SUP>1-3)</SUP>, but the mechanisms of selenite cataract formation are still unknown. Selenite is a strong sulfhydryl oxidant <I>in vitro</I><SUP>4)</SUP> and the aggregation of lens proteins by disulfide cross-links has been show to play an important role in cataract formation<SUP>5)</SUP>. A decrease in lens glutathione and a reduction in lens protein sulfhydryl have been reported in selenite-induced cataract<SUP>1, 6)</SUP>. David and Shearer<SUP>7)</SUP> reported that during selenite-induced cataractogenesis, there was no decrease in lens protein sulfhydryl content, though the loss of glutathione did occur. They claimed that the selenite-induced cataract could not be attributed to the reduction of lens sulfhydryl levels. As described above, explanations for the mechanism of selenite-induced cataract are controversial. Therefore, the purpose of this study is to further clarify the role of sulfhydryl oxidation in the cataract induced by selenite. Our findings are that inhibition of glutathione synthesis in rat pups induced by the intraperitoneal (i.p.) administration of buthionine sulfoximine (BSO) increased the cataract incidence caused by a single subcutaneous (s.c.) injection of selenite; furthermore, selenite induced the decomposition of lens protein and the formation of disulfide-linked lens protein aggregates <I>in vitro</I> and <I>in vivo</I>. Our data suggest that the oxidation of lens protein sulfhydryl by selenite is associated with selenite-induced cataractogenesis."},{"@value":"幼若ラットに亜セレン酸ナトリウムを1回皮下投与し,白内障を誘発した。この亜セレン酸誘発性水晶体毒性はグルタチオン合成抑制剤の前投与により増強された。SDS-ポリアクリアミドゲルを用いて,電気泳動分析した結果,白内障水晶体中に高分子蛋白凝集物質と蛋白分解物質の存在が分かった。さらに水溶性水晶体蛋白に亜セレン酸ナトリウムを加えて3日間インキュベションすると,水晶体蛋白液の混濁度の増加,SH基含量の減少および高分子蛋白凝集物質と蛋白分解物質の出現が認められた。<BR>以上の所見から,亜セレン酸ナトリウムは水晶体蛋白のSH基の酸化を起こし,水晶体蛋白の変性および不溶性高分子物質形成をもたらし,白内障を誘発するものと考えられる。"}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1410001206361322369","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000020218444"}],"foaf:name":[{"@language":"en","@value":"Guo Shin-Biao"},{"@language":"ja","@value":"郭 新彪"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Hygiene and Preventive Medicine, Faculty of Medicine, University of Tokyo"},{"@language":"ja","@value":"東京大学医学部衛生学教室"}]},{"@id":"https://cir.nii.ac.jp/crid/1030003658321864064","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"90165928"},{"@type":"NRID","@value":"1000090165928"},{"@type":"NRID","@value":"9000003097047"},{"@type":"NRID","@value":"9000005412503"},{"@type":"NRID","@value":"9000253090718"},{"@type":"NRID","@value":"9000020243277"},{"@type":"NRID","@value":"9000003094985"},{"@type":"NRID","@value":"9000020260176"},{"@type":"NRID","@value":"9000256591391"},{"@type":"NRID","@value":"9000253091131"},{"@type":"NRID","@value":"9000020175831"},{"@type":"NRID","@value":"9000256206174"},{"@type":"NRID","@value":"9000253090934"},{"@type":"NRID","@value":"9000253091119"},{"@type":"NRID","@value":"9000253236983"},{"@type":"NRID","@value":"9000008349281"},{"@type":"NRID","@value":"9000348627681"},{"@type":"NRID","@value":"9000020637818"},{"@type":"NRID","@value":"9000007460504"},{"@type":"NRID","@value":"9000253091796"},{"@type":"NRID","@value":"9000020218466"},{"@type":"NRID","@value":"9000253091095"},{"@type":"NRID","@value":"9000020776554"},{"@type":"NRID","@value":"9000253090968"},{"@type":"NRID","@value":"9000020131391"},{"@type":"NRID","@value":"9000020132698"},{"@type":"NRID","@value":"9000020270745"}],"foaf:name":[{"@language":"en","@value":"Manabe Shigeo"},{"@language":"ja","@value":"真鍋 重夫"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Hygiene and Preventive Medicine, Faculty of Medicine, University of Tokyo"},{"@language":"ja","@value":"東京大学医学部衛生学教室"}]},{"@id":"https://cir.nii.ac.jp/crid/1410001206361322370","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000020218475"}],"foaf:name":[{"@language":"en","@value":"Wada Osamu"},{"@language":"ja","@value":"和田 攻"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Hygiene and Preventive Medicine, Faculty of Medicine, University of Tokyo"},{"@language":"ja","@value":"東京大学医学部衛生学教室"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00215082"},{"@type":"EISSN","@value":"18826482"},{"@type":"NDL_BIB_ID","@value":"000000018201"},{"@type":"ISSN","@value":"00215082"},{"@type":"LISSN","@value":"00215082"},{"@type":"NCID","@value":"AN00185923"}],"prism:publicationName":[{"@language":"ja","@value":"日本衛生学雑誌"},{"@language":"en","@value":"Nippon Eiseigaku Zasshi (Japanese Journal of Hygiene)"},{"@language":"en","@value":"Jpn. J. Hyg."},{"@language":"ja","@value":"日衛誌"},{"@language":"en","@value":"Nippon Eiseigaku Zasshi (Japanese Journal of Hygiene), Jpn. J. Hyg."},{"@language":"ja","@value":"日本衛生学雑誌、日衛誌"},{"@language":"en","@value":"Nippon Eiseigaku Zasshi (Japanese Journal of Hygiene)"}],"dc:publisher":[{"@language":"en","@value":"The Japanese Society for Hygiene"},{"@language":"ja","@value":"一般社団法人日本衛生学会"}],"prism:publicationDate":"1989","prism:volume":"44","prism:number":"2","prism:startingPage":"615","prism:endingPage":"621"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","url":[{"@id":"http://id.ndl.go.jp/bib/3251118"},{"@id":"https://ndlsearch.ndl.go.jp/books/R000000004-I3251118"},{"@id":"http://www.jstage.jst.go.jp/article/jjh1946/44/2/44_2_615/_pdf"},{"@id":"https://search.jamas.or.jp/link/ui/1990154456"}],"availableAt":"1989","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Selenite","dc:title":"Selenite"},{"@id":"https://cir.nii.ac.jp/all?q=Cataract","dc:title":"Cataract"},{"@id":"https://cir.nii.ac.jp/all?q=Sulfhydryl%20oxidation","dc:title":"Sulfhydryl oxidation"},{"@id":"https://cir.nii.ac.jp/all?q=Electrophoresis","dc:title":"Electrophoresis"},{"@id":"https://cir.nii.ac.jp/all?q=%E4%BA%9C%E3%82%BB%E3%83%AC%E3%83%B3%E9%85%B8","dc:title":"亜セレン酸"},{"@id":"https://cir.nii.ac.jp/all?q=%E7%99%BD%E5%86%85%E9%9A%9C","dc:title":"白内障"},{"@id":"https://cir.nii.ac.jp/all?q=SH%E5%9F%BA%E9%85%B8%E5%8C%96","dc:title":"SH基酸化"},{"@id":"https://cir.nii.ac.jp/all?q=%E9%9B%BB%E6%B0%97%E6%B3%B3%E5%8B%95","dc:title":"電気泳動"}],"dataSourceIdentifier":[{"@type":"JALC","@value":"oai:japanlinkcenter.org:0017377431"},{"@type":"NDL_SEARCH","@value":"oai:ndlsearch.ndl.go.jp:R000000004-I3251118"},{"@type":"CROSSREF","@value":"10.1265/jjh.44.615"},{"@type":"PUBMED","@value":"2554035"},{"@type":"CIA","@value":"130000999616"},{"@type":"OPENAIRE","@value":"doi_dedup___::cedca24e29481b485f4c938ffb97e1d2"}]}