納豆菌の細胞融合　コロニーカウントによる融合の証明

DOI

書誌事項

タイトル別名
  • Cell fusion between Miura strain and Takahashi strain of Bacillus natto. Proof of fusion by plate-count method.
  • Proof of Fusion by Plate-Count Method
  • コロニーカウントによる融合の証明
公開日
1991
DOI
  • 10.3136/nskkk1962.38.79
公開者
社団法人 日本食品科学工学会

この論文をさがす

説明

Cell fusion was examined using commercial Natto bacteria. An important point is the discrimination of fusants. Following results were obtained; (1) the optimum tonicity for protoplast regeneration (PR) of Bacillus was 0.2M, less than half as the ordinary methods, (2) PR is limited when amino acid-negative mutants were used because of the limitation of casamino acids (CA) content, and (3) an easy plate-count method, namely plating directly in hypertonic media, was possible. Based on above results, nucleic acid-negative mutants (NNM) not affected by CA, and a new protoplast reversion medium containing a low content of sodium succinate, a sufficient amount of CA and a little biotine as well as polyvinylpyrrolidone, were employed. The frequency of back mutation of NNM of Natto bacteria was 10-6 level by the Miura strain with an adenine marker and 10-5 level by the Takahashi strain with an uracil marker. Their PR occured efficiently by the easy plate-count method. The cell fusion between them was shown by giving five proofs in one series of experiments; protoplast formation, its reversion, check of back mutation, control number of fusion and fusant number. The fusion frequency was approximately 0.02%.

収録刊行物

詳細情報 詳細情報について

  • CRID
    1390001206406817152
  • NII論文ID
    130003967841
  • DOI
    10.3136/nskkk1962.38.79
  • ISSN
    00290394
  • 本文言語コード
    ja
  • データソース種別
    • JaLC
    • Crossref
    • CiNii Articles
  • 抄録ライセンスフラグ
    使用不可

問題の指摘

ページトップへ