書誌事項
- タイトル別名
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- Rapid and Simple Determination of D-Glucose, D-Fructose and Sucrose in Food by Combination of Quinone-dependent Dehydrogenase Reactions and Self-driven Coulometry.
- コウソ ハンノウ ト クーロメトリー オ クミアワセタ ショクヒンチュウ ノ
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説明
A new method for determination of n-glucose, D-fructose and sucrose was developed by combining quinone-dependent dehydrogenase reactions and self-driven coulometry and applied for determination of sugars in food. Enzyme reaction was carried out in a mixture consisting of phosphate buffer (pH 6.5), ubiquinone-0 (Q0), quinone-dependent glucose dehydrogenase or fructose dehydrogenase. An aliquot of the reaction mixture was then added to the self-driven coulometric cell to determine the amount of reduced Q0 by coulometry. In a range of 0.1 to 6% (w/v) of D-glucose or D-fructose, the measured total charge was correlated with sugar concentration and corresponded to the theoretical total charges, suggesting that these sugars were determined without calibration. In case that glucose dehydrogenase and invertase were used, sucrose was determined. D-glucose, D-fructose and sucrose in various foods were determined by this method and the results were correlated with those determined by high-performance liquid chromatography and enzymatic method. Coefficient of variation of ten trials was about 2%. Total concentration of the three sugars was also rapidly determined and the results were correlated with the Somogyi's method (r=0.9983).
収録刊行物
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- 日本食品科学工学会誌
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日本食品科学工学会誌 42 (3), 169-175, 1995
公益社団法人 日本食品科学工学会
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詳細情報 詳細情報について
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- CRID
- 1390001206410274304
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- NII論文ID
- 10007579574
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- NII書誌ID
- AN10467499
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- ISSN
- 18816681
- 1341027X
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- NDL書誌ID
- 3601291
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可