Virulence characterization of <i>Campylobacter jejuni</i> isolated from resident wild birds in Tokachi area, Japan

  • SHYAKA Anselme
    Section of Food Microbiology and Immunology, Diagnostic Center for Animal Health and Food Safety, Obihiro University of Agriculture and Veterinary Medicine, 2–11 Inada, Obihiro, Hokkaido 080–8555, Japan University of Rwanda, the College of Agriculture and Veterinary Medicine, P.O. Box 210 Musanze, Rwanda
  • KUSUMOTO Akiko
    Section of Food Microbiology and Immunology, Diagnostic Center for Animal Health and Food Safety, Obihiro University of Agriculture and Veterinary Medicine, 2–11 Inada, Obihiro, Hokkaido 080–8555, Japan
  • CHAISOWWONG Warangkhana
    Section of Food Microbiology and Immunology, Diagnostic Center for Animal Health and Food Safety, Obihiro University of Agriculture and Veterinary Medicine, 2–11 Inada, Obihiro, Hokkaido 080–8555, Japan Department of Veterinary Biosciences and Veterinary Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Mae Hia, Muang, Chiang Mai 50100, Thailand
  • OKOUCHI Yoshiki
    Section of Food Microbiology and Immunology, Diagnostic Center for Animal Health and Food Safety, Obihiro University of Agriculture and Veterinary Medicine, 2–11 Inada, Obihiro, Hokkaido 080–8555, Japan
  • FUKUMOTO Shinya
    Research Unit for Vector Biology, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, 2–11 Inada, Obihiro, Hokkaido 080–8555, Japan
  • YOSHIMURA Aya
    Research Unit for Vector Biology, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, 2–11 Inada, Obihiro, Hokkaido 080–8555, Japan
  • KAWAMOTO Keiko
    Section of Food Microbiology and Immunology, Diagnostic Center for Animal Health and Food Safety, Obihiro University of Agriculture and Veterinary Medicine, 2–11 Inada, Obihiro, Hokkaido 080–8555, Japan

Bibliographic Information

Other Title
  • Public Health : Virulence characterization of Campylobacter jejuni isolated from resident wild birds in Tokachi area, Japan

Search this article

Description

The prevalence of Campylobacter jejuni in wild birds is a potential hazard for human and animal health. The aim of this study was to establish the prevalence of C. jejuni in wild birds in Tokachi area, Hokkaido, Japan and investigate their virulence in vitro. In total, 173 cloacal swabs from individual wild birds were collected for the detection of Campylobacter spp. Thirty four samples (19.7%) were positive for Campylobacter of which 94.1% (32/34 samples) were C. jejuni. Additionally, one C. coli and one C. fetus were isolated. Seven C. jejuni isolates (one from crows and the other from pigeons) had important virulence genes including all three CDT genes (cdtA, cdtB and cdtC) and flaA, flaB, ciaB and cadF, and the other isolates were lacking cdtA gene. Further studies on in vitro virulence-associated phenotypes, such as motility assay on soft agar and invasion assay in Caco-2 cells, were performed. The wild bird C. jejuni isolates adhered and invaded human cells. Although the numbers of viable intracellular bacteria of wild bird isolates were lower than a type strain NCTC11168, they persisted at 48-hr and underwent replication in host cells.

Journal

Citations (2)*help

See more

References(38)*help

See more

Details 詳細情報について

Report a problem

Back to top