尿中TotalEstrogenのRIAによる簡易測定法の開発

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タイトル別名
  • A Simple Method for Radioimmunoassay of Total Estrogen in Urine
  • ニョウチュウ Total Estrogen ノ RIA ニヨル カンイ ソクテ

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A simple and rapid radioimmunoassay (RIA) without extraction or purification was developed for Total Estrogen (conjugated and unconjugated estrogen) in urine from normal men and nonpregnant women.<BR>Antiserum used to RIA was produced by immunizing rabbits with estriol-16α-glucuronide (E2-16-G) -BSA conjugates. Antiserum to E3-16-G.BSA significantly cross-reacted with E1 (100%), E2 (100%), E3 (100%), E3-16-G (100%), and E3-17-G (100%) and did not react with other conjugated estrogen in urine.<BR>Urinary estrogen glucuronides and sulfates were gently hydrolyzed by β-glucuronidase from Helix pomatia juice for 2hr (48°C) without inhibition, and hydrolysis urine was directly applied to RIA.<BR>The results obtained from this direct method correlated well with the chromatographic purificating method, the extracting method and the Brawn-Kambegawa method (colorimetric method).<BR>This direct method is very useful for clinical application.

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