Slowly Growing Mycobacteriaの分類と同定 (第3報)

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書誌事項

タイトル別名
  • Classification and Identification of Slowly Growing Mycobacteria
  • Slowly growing mycobacteria ノ ブンルイ ト ドウテイ 3 Mycobacterium marinum
  • <i>Mycobacterium marinum</i>
  • III. <i>Mycobacterium marinum</i>

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説明

Characters of Mycobacterium marinum, based on tests of six strains, are as follows:<br>Colonial Morphology: Usually rough, but it may be smooth. Colonial pigmentation: Negative, when cultivated without light. Some strains are photochromogenic. Nitrate reduced. Two-week-arylsulfatase activity: Positive. Urease: Positive. Allantoinase: Usually positive. Other amidases of Bönicke's ten amidases, acetamidase, benzamidase, isonicotinamidase, nicotinamidase, pyrazinamidase, salicylamidase, succinamidase and malonamidase: Negative. Catalase: Positive. Peroxidase: Negative. Niacin: Negative.<br>Growth on 0.5mglml and 1.0mg/ml sodium salicylate-Ogawa egg medium: Positive. Growth on 0.2% PAS-Ogawa egg medium: Positive. Growth on 0.25mg/ml. NH2OH-HCl-Ogawa egg medium: Positive. Growth on 0.25mg/ml 8-azaguanine-Ogawa egg medium: Positive.<br>Growth rate: Slow; growth at 7 days on egg media; growth at 7 to 14 days on Sauton agar.<br>Growth at 28°C and 37°C, but no growth at 45°C.<br>Utilization of carbohydrates as sole carbon source: Acetate, citrate, succinate, malate, pyruvate, benzoate, malonate and fumarate not utilized; glucose, fructose, sucrose, ethanol, propanol, propylene glycol, 1, 3-, 1, 4- and 2, 3- butylene glycols not utilized; mannose, galactose, arabinose, xylose, rhamnose, trehalose, raffinose, inositol, mannitol and sorbitol not utilized in the presence of ammoniacal nitrogen. No acid from glucose, mannose, galactose, arabinose, xylose, rhamose, trehalose, raffinose, inositol and sorbitol.<br>Utilization of nitrogen compounds as sole, simultaneous carbon and nitrogen source; L-Glutamate utilized usually; L-serine, glucsamine-HCl, acetamide, benzamide, monoethanolamine and trimethylene diamine not utilized.<br>Utilization of nitrogen compounds as sole nitrogen source: L-Glutamate, urea, succinamide and nitrate utilized; L-serine, pyrazinamide and nicotinamide utilized by three of six strains; L-methionine, acetamide, benzamide, isonicotinamide and nitrite not utilized.<br>Two types of M. marinum were differentiated according to the pattern of utilization of carbohydrates, in the presence of glutamate-nitrogen or trimethylene diamine-nitrogen. The first biotype utilized (showed growth) glucose, acetate and pyruvate in the presence of glutamate-nitrogen and utilized acetate and pyruvate in the presence of trimethylene diaminenitrogen. To this type, three strains received as M. marinum and M. balnei belonged. Another biotype utilized none of glucose, fructose, sucrose, acetate, citrate, succinate, malate, pyruvate, malonate and fumarate in the presence of one of the above nitrogen sources. Three strains received as M. ranae, M. piscium and M. platypoecilus belonged to the latter type.

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