{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1390001206473373952.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1271/bbb.62.419"}},{"identifier":{"@type":"COI","@value":"1:CAS:528:DyaK1cXisFymtLs%3D"}},{"identifier":{"@type":"PMID","@value":"9571770"}},{"identifier":{"@type":"NDL_BIB_ID","@value":"4462622"}},{"identifier":{"@type":"URI","@value":"http://id.ndl.go.jp/bib/4462622"}},{"identifier":{"@type":"URI","@value":"https://ndlsearch.ndl.go.jp/books/R000000004-I4462622"}},{"identifier":{"@type":"URI","@value":"http://www.tandfonline.com/doi/pdf/10.1271/bbb.62.419"}},{"identifier":{"@type":"NAID","@value":"110002678993"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@language":"en","@value":"Immunological Detection and Quantification of Oxidized Proteins by Labelling with Digoxigenin"},{"@language":"ja-Kana","@value":"Immunological Detection and Quantificat"}],"dc:language":"en","description":[{"type":"abstract","notation":[{"@language":"en","@value":"An immunological assay, based on the digoxigenin/anti-digoxigenin system, was developed to detect and quantify carbonyl moieties that result from oxidative damage to proteins. Bovine serum albumin (BSA) was oxidized by a hydroxyl radical-generating system consisting of ascorbate/Fe(III)/O<sub>2</sub>. The resulting albumin-derived carbonyls were labelled with digoxigenin-hydrazide and detected by dot blotting with an anti-digoxigenin antibody conjugated to alkaline phosphatase. Quantification was carried out by a densitometric analysi. This system allows the detection of a pmole-amount of carbonyl groups on blots. The assay covers a range of sensitivity from 1.26 to 126 pmoles. Another feature of this method is its application to a complex protein mixture (homogenate) to analyze the oxidative status of individual proteins, as are shown for intestinal brush border membrane homogenate of a rat.<br>"}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1410001206473373953","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000004177578"}],"foaf:name":[{"@language":"en","@value":"BAUTISTA Juan"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Biochemistry, Bromatology and Toxicology, Faculty of Pharmacy, University of Sevilla"}]},{"@id":"https://cir.nii.ac.jp/crid/1410001206473373952","@type":"Researcher","foaf:name":[{"@language":"en","@value":"MATEOS-NEVADO María D."}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Biochemistry, Bromatology and Toxicology, Faculty of Pharmacy, University of Sevilla"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"09168451"},{"@type":"EISSN","@value":"13476947"},{"@type":"NDL_BIB_ID","@value":"000000151899"},{"@type":"ISSN","@value":"09168451"},{"@type":"LISSN","@value":"09168451"},{"@type":"NCID","@value":"AA10824164"}],"prism:publicationName":[{"@language":"en","@value":"Bioscience, Biotechnology, and Biochemistry"},{"@language":"ja","@value":"Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ"},{"@language":"en","@value":"Biosci. Biotechnol. Biochem."},{"@language":"en","@value":"Bioscience, Biotechnology, and Biochemistry"},{"@language":"ja","@value":"Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ"}],"dc:publisher":[{"@language":"en","@value":"Japan Society for Bioscience, Biotechnology, and Agrochemistry"},{"@language":"ja","@value":"公益社団法人 日本農芸化学会"}],"prism:publicationDate":"1998","prism:volume":"62","prism:number":"3","prism:startingPage":"419","prism:endingPage":"423"},"reviewed":"false","url":[{"@id":"http://id.ndl.go.jp/bib/4462622"},{"@id":"https://ndlsearch.ndl.go.jp/books/R000000004-I4462622"},{"@id":"http://www.tandfonline.com/doi/pdf/10.1271/bbb.62.419"}],"availableAt":"1998","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=oxidative%20modification","dc:title":"oxidative modification"},{"@id":"https://cir.nii.ac.jp/all?q=protein%20oxidation","dc:title":"protein oxidation"},{"@id":"https://cir.nii.ac.jp/all?q=immunological%20detection","dc:title":"immunological detection"},{"@id":"https://cir.nii.ac.jp/all?q=digoxigenin","dc:title":"digoxigenin"},{"@id":"https://cir.nii.ac.jp/all?q=aging","dc:title":"aging"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360292619833169280","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"A Role of Mixed‐Function Oxidation Reactions in the Accumulation of Altered Enzyme Forms During Aging"}]},{"@id":"https://cir.nii.ac.jp/crid/1360574095023185920","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"[49] Determination of carbonyl content in oxidatively modified proteins"}]},{"@id":"https://cir.nii.ac.jp/crid/1360855567871177216","@type":"Article","relationType":["references","cites"],"jpcoar:relatedTitle":[{"@value":"Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4"}]},{"@id":"https://cir.nii.ac.jp/crid/1361137044812761984","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Quantitation of aromatic residues in proteins: model compounds for second-derivative spectroscopy"}]},{"@id":"https://cir.nii.ac.jp/crid/1361699993699502464","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Dansyl hydrazine as a fluorimetric reagent for thin-layer chromatographic analysis of reducing sugars"}]},{"@id":"https://cir.nii.ac.jp/crid/1361699994995457920","@type":"Article","relationType":["references","cites"],"jpcoar:relatedTitle":[{"@value":"Description and application of an immunological detection system for analyzing glycoproteins on blots"}]},{"@id":"https://cir.nii.ac.jp/crid/1361699995929327104","@type":"Article","relationType":["references","cites"],"jpcoar:relatedTitle":[{"@value":"Protein Oxidation and Aging"}]},{"@id":"https://cir.nii.ac.jp/crid/1361699996162161664","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Derivatization of γ-glutamyl semialdehyde residues in oxidized proteins by fluoresceinamine"}]},{"@id":"https://cir.nii.ac.jp/crid/1361981469667489408","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Degradation of oxidatively denatured proteins in Escherichia coli"}]},{"@id":"https://cir.nii.ac.jp/crid/1361981471339504000","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Protein, Lipid and DNA Repair Systems in Oxidative Stress: The Free-Radical Theory of Aging Revisited"}]},{"@id":"https://cir.nii.ac.jp/crid/1362262944514481536","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Oxidation of proteins by mixed-function oxidation systems: implication in protein turnover, ageing and neutrophil function"}]},{"@id":"https://cir.nii.ac.jp/crid/1362544419590555520","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Conversion of Amino Acid Residues in Proteins and Amino Acid Homopolymers to Carbonyl Derivatives by Metal-catalyzed Oxidation Reactions"}]},{"@id":"https://cir.nii.ac.jp/crid/1362544420251924736","@type":"Article","relationType":["references","cites"],"jpcoar:relatedTitle":[{"@value":"Use of fluorescein hydrazide and fluorescein thiosemicarbazide reagents for the fluorometric determination of protein carbonyl groups and for the detection of oxidized protein on polyacrylamide gels"}]},{"@id":"https://cir.nii.ac.jp/crid/1362825895994650240","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Covalent modification reactions are marking steps in protein turnover"}]},{"@id":"https://cir.nii.ac.jp/crid/1363107369865197312","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Nonenzymatic cleavage of proteins by reactive oxygen species generated by dithiothreitol and iron."}]},{"@id":"https://cir.nii.ac.jp/crid/1363107371006204544","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Inactivation of glutamine synthetase by a purified rabbit liver microsomal cytochrome P-450 system"}]},{"@id":"https://cir.nii.ac.jp/crid/1363670318345108608","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Metal-catalyzed oxidation of proteins. Physiological consequences"}]},{"@id":"https://cir.nii.ac.jp/crid/1363951795021571584","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Implication of Lysine Residues in the Loss of Enzymatic Activity in Rat Liver 6-Phosphogluconate Dehydrogenase Found in Aging"}]},{"@id":"https://cir.nii.ac.jp/crid/1364233268517818112","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Oxidative modification of glutamine synthetase. I. Inactivation is due to loss of one histidine residue."}]},{"@id":"https://cir.nii.ac.jp/crid/1364233268577804672","@type":"Article","relationType":["references","cites"],"jpcoar:relatedTitle":[{"@value":"Determination of carbonyl groups in oxidatively modified proteins by reduction with tritiated sodium borohydride"}]},{"@id":"https://cir.nii.ac.jp/crid/1570009751756945024","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Metal-catalyzed oxidation of proteins"}]},{"@id":"https://cir.nii.ac.jp/crid/1570572699095516032","@type":"Article","relationType":["cites"]},{"@id":"https://cir.nii.ac.jp/crid/1570854174345499648","@type":"Article","relationType":["cites"]},{"@id":"https://cir.nii.ac.jp/crid/1570854176667876480","@type":"Article","relationType":["cites"]},{"@id":"https://cir.nii.ac.jp/crid/1570854176673965696","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Nonenzymatic cleavage of proteins by reactive oxygen species generated by dithiothreitol and iron."}]},{"@id":"https://cir.nii.ac.jp/crid/1570854176679069056","@type":"Article","relationType":["cites"]},{"@id":"https://cir.nii.ac.jp/crid/1571135651655936384","@type":"Article","relationType":["cites"]},{"@id":"https://cir.nii.ac.jp/crid/1571417124298921984","@type":"Article","relationType":["cites"]},{"@id":"https://cir.nii.ac.jp/crid/1571417126624969344","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Conversion of amino acid residues in proteins and amino acid homopolymers to carbonyl derivatives by metal-catalyzed oxidation reactions?"}]},{"@id":"https://cir.nii.ac.jp/crid/1571980074252342528","@type":"Article","relationType":["cites"]},{"@id":"https://cir.nii.ac.jp/crid/1571980074252343552","@type":"Article","relationType":["cites"]},{"@id":"https://cir.nii.ac.jp/crid/1572261551570432512","@type":"Article","relationType":["cites"]},{"@id":"https://cir.nii.ac.jp/crid/1572824499182474496","@type":"Article","relationType":["cites"]},{"@id":"https://cir.nii.ac.jp/crid/1573105973884234496","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Quantitation of aromatic residues in proteins: Model compounds for second-derivative spectroscopy"}]},{"@id":"https://cir.nii.ac.jp/crid/1573387451461729152","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Derivatization of gammaglutamyl semialdehyde residues in oxidized proteins by fluoresceinamine"}]},{"@id":"https://cir.nii.ac.jp/crid/1573668924112606336","@type":"Article","relationType":["cites"]},{"@id":"https://cir.nii.ac.jp/crid/1573950399089317120","@type":"Article","relationType":["cites"]},{"@id":"https://cir.nii.ac.jp/crid/1574231876209942656","@type":"Article","relationType":["cites"]}],"dataSourceIdentifier":[{"@type":"JALC","@value":"oai:japanlinkcenter.org:0008480533"},{"@type":"NDL_SEARCH","@value":"oai:ndlsearch.ndl.go.jp:R000000004-I4462622"},{"@type":"CROSSREF","@value":"10.1271/bbb.62.419"},{"@type":"PUBMED","@value":"9571770"},{"@type":"CIA","@value":"110002678993"}]}