Type 2 Antifreeze Protein from a Mid-latitude Freshwater Fish, Japanese Smelt(Hypomesus nipponensis)

DOI Web Site PubMed 被引用文献5件 参考文献35件
  • YAMASHITA Yasuhiro
    <i>Department of Biotechnology, Faculty of Engineering, Kansai University</i>
  • MIURA Rikako
    <i>Department of Biotechnology, Faculty of Engineering, Kansai University</i>
  • TAKEMOTO Yukari
    <i>Department of Biotechnology, Faculty of Engineering, Kansai University</i>
  • TSUDA Sakae
    <i>Research Institute of Biological Resources, National Institute of Advanced Industrial Science and Technology (AIST)</i>
  • KAWAHARA Hidehisa
    <i>Department of Biotechnology, Faculty of Engineering, Kansai University</i>
  • OBATA Hitoshi
    <i>Department of Biotechnology, Faculty of Engineering, Kansai University</i>

書誌事項

タイトル別名
  • Type II Antifreeze Protein from a Mid-latitude Freshwater Fish, Japanese Smelt (<i>Hypomesus nipponensis</i>)
  • Type II Antifreeze Protein from a Mid-latitude Freshwater Fish, Japanese Smelt (Hypomesus nipponensis)

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  A lot of reports of antifreeze protein (AFP) from fish have been published, but no report has mentioned of commercialized mid-latitude fresh water fish which producing AFP in its body fluid. We found that the AFP in the body fluid of Japanese smelt (Hypomesus nipponensis) from mid-latitude fresh water was purified and characterized. The N-terminal amino acid sequence of the Japanese smelt AFP was 75.0% identical to Type II AFP from herring. Results of EDTA treatment and ruthenium red staining suggested that the Japanese smelt AFP had at least one Ca2+-binding domain. Interestingly, the antifreeze activity of the Japanese smelt AFP did not completely disappear when Ca2+ ions were removed. The molecular mass of the Japanese smelt AFP was calculated to be 16,756.8 by the TOF-mass analysis. The Open reading flame of the gene coding for the Japanese smelt AFP was 444 bp long and was 85.0% identical with the entire herring AFP gene. The cDNA and amino acid sequence of the Japanese smelt AFP were the same length as those of herring AFP.<br>

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