Dietary Requirements of Protein and Amino Acids

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  • Kido Yasuhiro
    Laboratory of Nutrition Science, Division of Applied Life Science, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University

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  • たんぱく質・アミノ酸の必要量に関する研究
  • タンパクシツ ・ アミノサン ノ ヒツヨウリョウ ニ カンスル ケンキュウ

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Even when energy intake is adequate, the classic protein deficiency disease kwashiorkor increases susceptibility to infection. The protein requirement defines the requirement in terms of the needs to maintain the physical structure and body functions, i.e. metabolic demands, and the dietary protein requirement will satisfy those demands. Protein requirement is generally determined by nitrogen balance studies, but various limitations are associated with this method. The indicator amino acid oxidation (IAAO) method, with a theoretical foundation quite different from that of the nitrogen balance method, was developed as an alternative for studies in animals and humans. We employed the IAAO technique to evaluate dietary protein requirements and protein quality in rats and healthy men. The results indicated that the IAAO method is effective for evaluating the dietary protein requirements for people of all ages and for postoperative patients or those with injuries or infections, all of who represent a wide range of metabolic demand. This method could also be used to evaluate protein quality.

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