Environmental biosafety assessment on transgenic Oncidium orchid modified by RNA interference of Phytoene Synthase genes

  • Ko Swee-Suak
    Agricultural Biology Research Center
  • Liu Yao-Chung
    Institute of Plant Biology, National Taiwan University
  • Chung Mei-Chu
    Institute of Plant and Microbiology, Academia Sinica
  • Shih Ming-Che
    Agricultural Biology Research Center
  • Mohammadmehdi Habibi
    Graduate School of Life and Environmental Sciences, University of Tsukuba
  • Oguchi Taichi
    Faculty of Life and Environmental Sciences, University of Tsukuba Tsukuba Plant Innovation Research Center, University of Tsukuba
  • Watanabe Kazuo N.
    Faculty of Life and Environmental Sciences, University of Tsukuba Tsukuba Plant Innovation Research Center, University of Tsukuba
  • Yeh Kai-Wun
    Institute of Plant Biology, National Taiwan University

書誌事項

タイトル別名
  • Environmental biosafety assessment on transgenic <i>Oncidium</i> orchid modified by RNA interference of <i>Phytoene Synthase</i> genes

この論文をさがす

抄録

<p>Hybrid Oncidium orchids, such as Oncidium Gower Ramsey and Oncidium “Honey Angel,” are popular cut flowers in Japan and Taiwan. Due to pollen sterility, no new varieties have been created by conventional breeding methods. Recently, we employed RNA interference (RNAi) technology to suppress phytoene synthase and successfully modified floret hue from yellow to white (Liu et al. 2019). Transgenic white Oncidium orchids, Honey Snow MF-1, have been grown to test their genetic stability, and their environmental biosafety was assessed for approximately one year under government regulatory instructions from the Council of Agriculture, Taiwan. In the present study, pollen sterility was demonstrated by cytological observation of the microsporogenesis step, pollen morphology abortion, and failure of pollen germination. Assays on allelopathic effect on the other plants and the soil rhizospheric microbial flora-revealed that transgenic Oncidium orchids are potentially safe with regard to environmental biodiversity. Therefore, the general release permissions have been granted and an application for licensing for commercial production is under way.</p>

収録刊行物

被引用文献 (2)*注記

もっと見る

参考文献 (11)*注記

もっと見る

詳細情報

問題の指摘

ページトップへ