【4/18更新】CiNii ArticlesのCiNii Researchへの統合について

SiN多孔膜を有するマイクロウェルを用いた単一ニューロンの長期培養

  • 善明 祐介
    九州工業大学 大学院生命体工学研究科
  • 森迫 勇
    九州工業大学 大学院生命体工学研究科
  • 安田 隆
    九州工業大学 大学院生命体工学研究科

書誌事項

タイトル別名
  • Long-term Culture of a Single Neuron using a Microwell with a Porous SiN Membrane

抄録

<p>For long-term culture of a single neuron, many astrocytes and a single neuron were co-cultured on both sides of a 1 µm thick silicon nitride membrane which has many microholes of 3 µm in diameter. After astrocytes were cultured on the back side of the membrane for 1 week, a neuron was seeded on the front side of the membrane of a microwell bottom and cultured for 5 weeks. By fluorescently staining VGLUT1 (vesicular glutamate transporter 1) which is localized at a presynaptic terminal, we found that the number of synapses formed by a single neuron drastically increases within the first 2 weeks and keeps high values for weeks afterward.</p>

収録刊行物

参考文献 (5)*注記

もっと見る

関連プロジェクト

もっと見る

詳細情報

問題の指摘

ページトップへ