書誌事項
- タイトル別名
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- Long-term Culture of a Single Neuron using a Microwell with a Porous SiN Membrane
抄録
<p>For long-term culture of a single neuron, many astrocytes and a single neuron were co-cultured on both sides of a 1 µm thick silicon nitride membrane which has many microholes of 3 µm in diameter. After astrocytes were cultured on the back side of the membrane for 1 week, a neuron was seeded on the front side of the membrane of a microwell bottom and cultured for 5 weeks. By fluorescently staining VGLUT1 (vesicular glutamate transporter 1) which is localized at a presynaptic terminal, we found that the number of synapses formed by a single neuron drastically increases within the first 2 weeks and keeps high values for weeks afterward.</p>
収録刊行物
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- 電気学会論文誌Ｅ（センサ・マイクロマシン部門誌）
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電気学会論文誌Ｅ（センサ・マイクロマシン部門誌） 138 (7), 327-328, 2018-07-01
一般社団法人 電気学会
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詳細情報 詳細情報について
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- CRID
- 1390001288042209280
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- NII論文ID
- 130007386996
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- ISSN
- 13475525
- 13418939
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可