{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1390001288118931840.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1248/bpb.b18-00285"}},{"identifier":{"@type":"PMID","@value":"30429411"}},{"identifier":{"@type":"NDL_BIB_ID","@value":"029481787"}},{"identifier":{"@type":"URI","@value":"http://id.ndl.go.jp/bib/029481787"}},{"identifier":{"@type":"URI","@value":"https://ndlsearch.ndl.go.jp/books/R000000004-I029481787"}},{"identifier":{"@type":"URI","@value":"https://www.jstage.jst.go.jp/article/bpb/42/2/42_b18-00285/_pdf"}},{"identifier":{"@type":"NAID","@value":"130007589140"}},{"identifier":{"@type":"URI","@value":"https://search.jamas.or.jp/link/ui/2019253957"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@language":"en","@value":"Effects of Ghrelin on the Apoptosis of Rheumatoid Arthritis Fibroblast-Like Synoviocyte MH7A Cells"}],"dc:language":"en","description":[{"type":"abstract","notation":[{"@language":"en","@value":"<p>Ghrelin is a circulating peptide hormone, which involved in promoting feeding and regulating energy metabolism in human and rodents. Abnormal synovial hyperplasia is the most important pathologic hallmark of rheumatoid arthritis (RA), which is characterised by tumor-like expansion. Existing studies indicated that there may exist some relation between the decreased ghrelin and the abnormally proliferating synovial cells in RA. Therefore, the aim of this study is to explore the apoptotic effects of ghrelin on MH7A synovial cells <i>in vitro</i>. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to evaluate the effects of ghrelin on the viability of MH7A cells. Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-biotin nick-end labeling (TUNEL) and flow cytometry were used to test the apoptotic effects of ghrelin. At last, Western blot and real-time PCR were performed to explore the expression of caspases-8, -9, and -3 after the treatment of ghrelin. MTT experiments showed that ghrelin could inhibit viability of MH7A cells. The results of flow cytometry and TUNEL showed that ghrelin could induce apoptosis of MH7A synovial cells. Western blot showed that expression of cleaved-caspases-8, -9, and -3 were increased in ghrelin stimulation group compared with the control group, while expression of pro-caspases-8, -9, and -3 had no significant difference. In mRNA levels, ghrelin can decrease pro-caspases-8, -9, and -3 mRNA expression, which confirmed the results of protein levels. Then these apoptotic effects were significantly reversed by [D-Lys<sub>3</sub>] GHRP-6 (ghrelin receptor antagonist). This study found that ghrelin can induce apoptosis of MH7A cells through caspase signaling pathways.</p>"}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1410001288118931845","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000399521324"}],"foaf:name":[{"@language":"en","@value":"Zhang Jinshan"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Human Anatomy and Histology and Embryology, the Fourth Military Medical University (Air Force Medical University)"}]},{"@id":"https://cir.nii.ac.jp/crid/1410001288118931843","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000399521319"}],"foaf:name":[{"@language":"en","@value":"Ma Junxian"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Rheumatology and Immunology, Tangdu Hospital of the Fourth Military Medical University (Air Force Medical University)"}]},{"@id":"https://cir.nii.ac.jp/crid/1410001288118931840","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000399521320"}],"foaf:name":[{"@language":"en","@value":"Wang Xinbo"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Rheumatology and Immunology, Tangdu Hospital of the Fourth Military Medical University (Air Force Medical University)"}]},{"@id":"https://cir.nii.ac.jp/crid/1410001288118931968","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000399521325"}],"foaf:name":[{"@language":"en","@value":"Zhang Yan"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Rheumatology and Immunology, Tangdu Hospital of the Fourth Military Medical University (Air Force Medical University)"}]},{"@id":"https://cir.nii.ac.jp/crid/1410001288118931844","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000399521321"}],"foaf:name":[{"@language":"en","@value":"Lv Tingting"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Rheumatology and Immunology, Tangdu Hospital of the Fourth Military Medical University (Air Force Medical University)"}]},{"@id":"https://cir.nii.ac.jp/crid/1410001288118931841","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000399521322"}],"foaf:name":[{"@language":"en","@value":"Liu Jie"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Rheumatology and Immunology, Tangdu Hospital of the Fourth Military Medical University (Air Force Medical University)"}]},{"@id":"https://cir.nii.ac.jp/crid/1410001288118931842","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000399521323"}],"foaf:name":[{"@language":"en","@value":"Ren Ying"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Rheumatology and Immunology, Tangdu Hospital of the Fourth Military Medical University (Air Force Medical University)"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"09186158"},{"@type":"EISSN","@value":"13475215"},{"@type":"NDL_BIB_ID","@value":"000000153250"},{"@type":"ISSN","@value":"09186158"},{"@type":"LISSN","@value":"09186158"},{"@type":"NCID","@value":"AA10885497"}],"prism:publicationName":[{"@language":"ja","@value":"Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ"},{"@language":"en","@value":"Biological and Pharmaceutical Bulletin"},{"@language":"en","@value":"Biological & Pharmaceutical Bulletin"},{"@language":"en","@value":"Biol. Pharm. Bull."},{"@language":"ja","@value":"Ｂｉｏｌ．　Ｐｈａｒｍ．　Ｂｕｌｌ．"}],"dc:publisher":[{"@language":"en","@value":"The Pharmaceutical Society of Japan"},{"@language":"ja","@value":"公益社団法人 日本薬学会"}],"prism:publicationDate":"2019-02-01","prism:volume":"42","prism:number":"2","prism:startingPage":"158","prism:endingPage":"163"},"reviewed":"false","url":[{"@id":"http://id.ndl.go.jp/bib/029481787"},{"@id":"https://ndlsearch.ndl.go.jp/books/R000000004-I029481787"},{"@id":"https://www.jstage.jst.go.jp/article/bpb/42/2/42_b18-00285/_pdf"},{"@id":"https://search.jamas.or.jp/link/ui/2019253957"}],"availableAt":"2019-02-01","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=rheumatoid%20arthritis","dc:title":"rheumatoid arthritis"},{"@id":"https://cir.nii.ac.jp/all?q=fibroblast-like%20synoviocyte","dc:title":"fibroblast-like synoviocyte"},{"@id":"https://cir.nii.ac.jp/all?q=ghrelin","dc:title":"ghrelin"},{"@id":"https://cir.nii.ac.jp/all?q=apoptosis","dc:title":"apoptosis"},{"@id":"https://cir.nii.ac.jp/all?q=rheumatoid%20arthritis","dc:title":"rheumatoid arthritis"},{"@id":"https://cir.nii.ac.jp/all?q=fibroblast-like%20synoviocyte","dc:title":"fibroblast-like synoviocyte"},{"@id":"https://cir.nii.ac.jp/all?q=ghrelin","dc:title":"ghrelin"},{"@id":"https://cir.nii.ac.jp/all?q=apoptosis","dc:title":"apoptosis"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360011145190294016","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Inactivated Sendai Virus Strain Tianjin Induces Apoptosis in Human Breast Cancer MDA-MB-231 Cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1360011145512192128","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Upregulation and nuclear translocation of testicular ghrelin protects differentiating spermatogonia from ionizing radiation injury"}]},{"@id":"https://cir.nii.ac.jp/crid/1360292618971159296","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Ghrelin reduces liver impairment in a model of concanavalin A-induced acute hepatitis in mice"}]},{"@id":"https://cir.nii.ac.jp/crid/1360292621277874048","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Ghrelin attenuates brain injury in septic mice via PI3K/Akt signaling activation"}]},{"@id":"https://cir.nii.ac.jp/crid/1360574094741576576","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Serum ghrelin in female patients with rheumatoid arthritis during treatment with infliximab"}]},{"@id":"https://cir.nii.ac.jp/crid/1360855569583094016","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Therapeutic effect of cortistatin on experimental arthritis by downregulating inflammatory and Th1 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N-Terminal Kinase and Wnt/β-Catenin Signaling and Suppresses Lung Inflammation"}]},{"@id":"https://cir.nii.ac.jp/crid/1361981469534696960","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Ghrelin attenuates the growth of HO-8910 ovarian cancer cells through the ERK pathway"}]},{"@id":"https://cir.nii.ac.jp/crid/1361981469859637888","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Effect of CD95 on inflammatory response in rheumatoid arthritis fibroblast-like synoviocytes"}]},{"@id":"https://cir.nii.ac.jp/crid/1361981471416722816","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The interplay between inflammation and metabolism in rheumatoid arthritis"}]},{"@id":"https://cir.nii.ac.jp/crid/1362262943818035712","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Ghrelin is a growth-hormone-releasing acylated peptide from stomach"}]},{"@id":"https://cir.nii.ac.jp/crid/1362262944272686976","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Ghrelin Inhibits Oligodendrocyte Cell Death by Attenuating Microglial Activation"}]},{"@id":"https://cir.nii.ac.jp/crid/1362262945708528384","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Ghrelin Protects against Dexamethasone-Induced INS-1 Cell Apoptosis via ERK and p38MAPK Signaling"}]},{"@id":"https://cir.nii.ac.jp/crid/1362825895131768320","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Ghrelin and obestatin expression in serous ovarian tumours"}]},{"@id":"https://cir.nii.ac.jp/crid/1362825895841838720","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Upregulation of miR-21 by Ghrelin Ameliorates Ischemia/Reperfusion-Induced Acute Kidney Injury by Inhibiting Inflammation and Cell Apoptosis"}]},{"@id":"https://cir.nii.ac.jp/crid/1363107368419982208","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Alterations of Plasma Ghrelin Levels in Rats with Lipopolysaccharide-Induced Wasting Syndrome and Effects of Ghrelin Treatment on the Syndrome"}]},{"@id":"https://cir.nii.ac.jp/crid/1363107368892858368","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Selective modulation of MAPKs contribute to the anti-proliferative and anti-inflammatory activities of 1,7-dihydroxy-3,4-dimethoxyxanthone in rheumatoid arthritis-derived fibroblast-like synoviocyte MH7A cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1363670318907790592","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Anti-tumour necrosis factor α therapy modulates ghrelin in patients with severe rheumatoid arthritis"}]},{"@id":"https://cir.nii.ac.jp/crid/1363951793999959168","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Chronic inflammation modulates ghrelin levels in humans and rats"}]},{"@id":"https://cir.nii.ac.jp/crid/1363951794541523712","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Caspases: A Molecular Switch Node in the Crosstalk between Autophagy and Apoptosis"}]},{"@id":"https://cir.nii.ac.jp/crid/1363951795875545600","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Ghrelin inhibits LPS-induced release of IL-6 from mouse dopaminergic neurones"}]},{"@id":"https://cir.nii.ac.jp/crid/1364233268471386624","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Ghrelin gene products, receptors, and GOAT enzyme: biological and pathophysiological insight"}]},{"@id":"https://cir.nii.ac.jp/crid/1364233269782273024","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Ghrelin levels in patients with juvenile idiopathic arthritis: relation to anti–tumor necrosis factor treatment and disease activity"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204930137600","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Establishment and Characterization of a Novel Human Rheumatoid Fibroblast-Like Synoviocyte Line, MH7A, Immortalized with SV40 T Antigen."},{"@value":"Establishment and characterization of novel human rheumatoid fibroblast-like synoviocyte line, MH7A, immortalized with SV40T antigen"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679608446336","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Dihydrotestosterone Inhibits Interleukin-1.ALPHA. or Tumor Necrosis Factor .ALPHA.-Induced Proinflammatory Cytokine Production via Androgen Receptor-Dependent Inhibition of Nuclear Factor-.KAPPA.B Activation in Rheumatoid Fibroblast-Like Synovial Cell Line"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282681275622016","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@language":"en","@value":"The regulation of circulating ghrelin — with recent updates from cell-based assays [Review]"},{"@value":"The regulation of circulating ghrelin &mdash; with recent updates from cell-based assays [Review]"},{"@value":"The regulation of circulating ghrelin - with recent updates from cell-based assays [Review]"},{"@value":"The regulation of circulating ghrelin - with recent updates from cell-based assay"}]}],"dataSourceIdentifier":[{"@type":"JALC","@value":"oai:japanlinkcenter.org:2007062717"},{"@type":"NDL_SEARCH","@value":"oai:ndlsearch.ndl.go.jp:R000000004-I029481787"},{"@type":"CROSSREF","@value":"10.1248/bpb.b18-00285"},{"@type":"PUBMED","@value":"30429411"},{"@type":"CIA","@value":"130007589140"}]}