Scanning RNA-Seq and RAD-Seq approach to develop SNP markers closely linked to <i>MALE STERILITY 1</i> (<i>MS1</i>) in <i>Cryptomeria japonica</i> D. Don
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- Ueno Saneyoshi
- Department of Forest Molecular Genetics and Biotechnology, Forestry and Forest Products Research Institute, Forest Research and Management Organization
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- Uchiyama Kentaro
- Department of Forest Molecular Genetics and Biotechnology, Forestry and Forest Products Research Institute, Forest Research and Management Organization
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- Moriguchi Yoshinari
- Graduate School of Science and Technology, Niigata University
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- Ujino-Ihara Tokuko
- Department of Forest Molecular Genetics and Biotechnology, Forestry and Forest Products Research Institute, Forest Research and Management Organization
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- Matsumoto Asako
- Department of Forest Molecular Genetics and Biotechnology, Forestry and Forest Products Research Institute, Forest Research and Management Organization
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- Wei Fu-Jin
- Department of Forest Molecular Genetics and Biotechnology, Forestry and Forest Products Research Institute, Forest Research and Management Organization
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- Saito Maki
- Toyama Prefectural Agricultural Forestry and Fisheries Research Center, Forestry Research Institute
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- Higuchi Yumi
- Niigata Prefectural Forest Research Institute
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- Futamura Norihiro
- Department of Forest Molecular Genetics and Biotechnology, Forestry and Forest Products Research Institute, Forest Research and Management Organization
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- Kanamori Hiroyuki
- National Institute of Agrobiological Sciences
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- Katayose Yuichi
- National Institute of Agrobiological Sciences
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- Tsumura Yoshihiko
- Department of Forest Molecular Genetics and Biotechnology, Forestry and Forest Products Research Institute, Forest Research and Management Organization Faculty of Life and Environmental Sciences, University of Tsukuba
Bibliographic Information
- Other Title
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- Scanning RNA-Seq and RAD-Seq approach to develop SNP markers closely linked to MALE STERILITY 1 (MS1) in Cryptomeria japonica D. Don
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Description
<p>Cryptomeria japonica is a major forestry tree species in Japan. Male sterility of the species is caused by a recessive gene, which shows dysfunction of pollen development and results in no dispersed pollen. Because the pollen of C. japonica induces pollinosis, breeding of pollen-free C. japonica is desired. In this study, single nucleotide polymorphism (SNP) markers located at 1.78 and 0.58 cM to a male sterility locus (MS1) were identified from an analysis of RNA-Seq and RAD-Seq, respectively. SNPs closely linked to MS1 were first scanned by a method similar to MutMap, where a type of index was calculated to measure the strength of the linkage between a marker sequence and MS1. Linkage analysis of selected SNP markers confirmed a higher efficiency of the current method to construct a partial map around MS1. Allele-specific PCR primer pair for the most closely linked SNP with MS1 was developed as a codominant marker, and visualization of the PCR products on an agarose gel enabled rapid screening of male sterile C. japonica. The allele-specific primers developed in this study would be useful for establishing the selection of male sterile C. japonica.</p>
Journal
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- Breeding Science
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Breeding Science 69 (1), 19-29, 2019
Japanese Society of Breeding
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Details 詳細情報について
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- CRID
- 1390001288131785216
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- NII Article ID
- 130007636129
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- NII Book ID
- AA11317194
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- ISSN
- 13473735
- 13447610
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- NDL BIB ID
- 029672215
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL Search
- Crossref
- CiNii Articles
- OpenAIRE
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- Abstract License Flag
- Disallowed