Quantification of Nτ-methylhistidine and Nπ-methylhistidine in chicken plasma by liquid chromatography–tandem mass spectrometry

DOI Web Site 被引用文献2件 参考文献26件 オープンアクセス
  • Shiraishi Jun-ichi
    Graduate School of Applied Life Science, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino, Tokyo 180-8602, Japan
  • Ijiri Daichi
    Graduate School of Agriculture, Forestry and Fisheries, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan The United Graduate School of Agricultural Sciences, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan
  • Katafuchi Ayumi
    Graduate School of Agriculture, Forestry and Fisheries, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan
  • Tomonaga Shozo
    Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan
  • Shimamoto Saki
    Graduate School of Science and Technology, Niigata University, 8050 Ikarashi 2-no-cho, Nishi-ku, Niigata 950-2181, Japan
  • Do Hanwool
    Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan
  • Ishihara Shinya
    Graduate School of Applied Life Science, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino, Tokyo 180-8602, Japan
  • Ohtsuka Akira
    Graduate School of Agriculture, Forestry and Fisheries, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan The United Graduate School of Agricultural Sciences, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan

書誌事項

タイトル別名
  • Quantification of N<sup>τ</sup> -Methylhistidine and N<sup>π</sup>-Methylhistidine in Chicken Plasma by Liquid Chromatography–Tandem Mass Spectrometry
公開日
2023
資源種別
journal article
DOI
  • 10.2141/jpsa.2023017
公開者
日本家禽学会

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説明

<p>The concentration of Nτ-methylhistidine in plasma provides an index of skeletal muscle protein breakdown. This study aimed to establish a quantitative method for measuring the concentrations of Nτ-methylhistidine and its isomer Nπ-methylhistidine in chicken plasma, using liquid chromatography–tandem mass spectrometry with stable isotope dilution analysis. The acceptable linear ranges of detection were 1.56–50.00 μmol/L for Nτ-methylhistidine and 0.78–25.00 μmol/L for Nπ-methylhistidine. The proposed method detected changes in the plasma levels of Nτ-methylhistidine and Nπ-methylhistidine in response to fasting and re-feeding. These results suggest that the method developed in this study can be used for the simultaneous measurement of Nτ-methylhistidine and Nπ-methylhistidine in chicken plasma.</p>

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