[Background] Contaminated healthcare environments are potential sources of infection spread, necessitating regular cleaning and disinfection. Recently, there has been a growing interest in environmental disinfection through indoor sterilization devices. Herein, we compared the sterilization effectiveness of two indoor sterilization devices: the Pulsed Xenon Ultraviolet (PX-UV) irradiation device and the ozone and hydrogen peroxide indoor sterilization device (Ozone-H2O2).
[Methods] We adjusted the inoculum concentration of seven pathogenic microorganisms, including spore-forming and multidrug-resistant bacteria relevant in healthcare settings, between 104 and 108 CFU/mL. We inoculated 10 μL of the microbial suspension onto Mueller-Hinton (MH) agar. The inoculated agar plates were tilted at a 45#176; angle, and we subjected them to 5 and 10-min PX-UV irradiation. Further, we conducted environmental sterilization using ozone-H2O2. The inoculated agar plates treated with PX-UV and ozone-H2O2 were incubated, and bacterial growth was assessed using a five-point scale.
[Results] After 5 min of PX-UV irradiation, bacterial growth was observed for Bacillus cereus and Candida parapsilosis. Following 10 min of PX-UV irradiation, minimal bacterial growth was observed for B. cereus. However, no bacteria were detected for all tested bacterial species after exposure to ozone-H2O2.
[Conclusion] In the case of PX-UV indoor sterilization, there was a tendency for spore-forming bacteria and Candida species to be less effectively sterilized.
"},{"@language":"ja","@value":"【背景】感染拡大の原因として汚染した医療環境がリザーバーとなる場合が考えられ，定期的に清掃および消毒を実施する必要がある．最近，室内殺菌装置による環境消毒が注目されてきている．今回，パルスドキセノン紫外線照射装置（Pulsed Xenon Ultravioret：PX-UV）とオゾン・過酸化水素室内殺菌装置（オゾン・H2O2）の2種類の室内殺菌装置の殺菌効果の比較検討を行った．
【方法】医療環境で問題となる7種類の芽胞菌や多剤耐性菌を含む病原微生物を接種濃度104～108 CFU/mLに調整した菌液をミューラーヒントン（MH）培地に10 uL接種した．接種培地を平面から45°に傾け，PX-UVで5分照射と10分照射を行った．また，オゾン・H2O2での環境殺菌を実施した．PX-UVとオゾン・H2O2で殺菌した接種培地を培養し，菌の発育状態を5段階で判定した．
【結果】PX-UV 5分間の照射では，Bacillus cereusとCandida parapsilosisにおいて，菌の発育を認めた．PX-UV 10分間の照射では，B. cereusで少数の菌の発育を認めた．オゾン・H2O2では全ての菌種において菌は検出されなかった．
【結論】PX-UVにおいて，芽胞菌とカンジタ属が残存しやすい傾向にあった．
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嘉子"}],"jpcoar:affiliationName":[{"@language":"ja","@value":"九州大学病院グローバル感染症センター"},{"@language":"en","@value":"Center for the Study of Global Infection, Kyushu University Hospital"}]},{"@id":"https://cir.nii.ac.jp/crid/1410019457532534656","@type":"Researcher","foaf:name":[{"@language":"en","@value":"MORI Hitomi"},{"@language":"ja","@value":"森 日登美"}],"jpcoar:affiliationName":[{"@language":"ja","@value":"九州大学病院看護部"},{"@language":"en","@value":"Department of Nursing, Kyushu University Hospital"}]},{"@id":"https://cir.nii.ac.jp/crid/1410019457532534784","@type":"Researcher","foaf:name":[{"@language":"en","@value":"KIYOSUKE Makiko"},{"@language":"ja","@value":"清祐 麻紀子"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Hospital"},{"@language":"ja","@value":"九州大学病院検査部"}]},{"@id":"https://cir.nii.ac.jp/crid/1410019457532534786","@type":"Researcher","foaf:name":[{"@language":"en","@value":"SHIMONO Nobuyuki"},{"@language":"ja","@value":"下野 信行"}],"jpcoar:affiliationName":[{"@language":"ja","@value":"九州大学病院グローバル感染症センター"},{"@language":"en","@value":"Center for the Study of Global Infection, Kyushu University Hospital"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"1882532X"},{"@type":"EISSN","@value":"18832407"}],"prism:publicationName":[{"@language":"en","@value":"Japanese Journal of Infection Prevention and Control"},{"@language":"ja","@value":"日本環境感染学会誌"},{"@language":"en","@value":"Japanese Journal of Environmental Infections"},{"@language":"en","@value":"Nihon Kankyou Kansen Gakkaishi"},{"@language":"ja","@value":"環境感染誌"}],"dc:publisher":[{"@language":"en","@value":"Japanese Society for Infection Prevention and Control"},{"@language":"ja","@value":"一般社団法人 日本環境感染学会"}],"prism:publicationDate":"2024-01-25","prism:volume":"39","prism:number":"1","prism:startingPage":"15","prism:endingPage":"19"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","url":[{"@id":"https://www.jstage.jst.go.jp/article/jsei/39/1/39_15/_pdf"},{"@id":"https://search.jamas.or.jp/link/ui/2024288222"}],"availableAt":"2024-01-25","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=%E5%AE%A4%E5%86%85%E6%AE%BA%E8%8F%8C%E8%A3%85%E7%BD%AE","dc:title":"室内殺菌装置"},{"@id":"https://cir.nii.ac.jp/all?q=%E3%83%91%E3%83%AB%E3%82%B9%E3%83%89%E3%82%AD%E3%82%BB%E3%83%8E%E3%83%B3%E7%B4%AB%E5%A4%96%E7%B7%9A","dc:title":"パルスドキセノン紫外線"},{"@id":"https://cir.nii.ac.jp/all?q=%E3%82%AA%E3%82%BE%E3%83%B3%E3%83%BB%E9%81%8E%E9%85%B8%E5%8C%96%E6%B0%B4%E7%B4%A0","dc:title":"オゾン・過酸化水素"},{"@id":"https://cir.nii.ac.jp/all?q=%E7%92%B0%E5%A2%83%E6%B8%85%E6%8E%83","dc:title":"環境清掃"},{"@id":"https://cir.nii.ac.jp/all?q=indoor%20sterilization%20device","dc:title":"indoor 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