Non-Cytotoxic Photodynamic Therapy with Talaporfin Sodium Reduces the Expression of CXCR4 and Enhances Chemotherapeutic Efficacy in Undifferentiated Gastric Cancer Cell Line HGC27

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  • Kai Kengo
    Department of Anatomy, Histochemistry and Cell Biology, Faculty of Medicine, University of Miyazaki Department of Surgery, Faculty of Medicine, University of Miyazaki
  • Hishikawa Yoshitaka
    Department of Anatomy, Histochemistry and Cell Biology, Faculty of Medicine, University of Miyazaki Frontier Science Research Center, Faculty of Medicine, University of Miyazaki
  • Nanashima Atsushi
    Department of Surgery, Faculty of Medicine, University of Miyazaki
  • Mori Ryoma
    Department of Applied Chemistry, Faculty of Engineering, University of Miyazaki
  • Matsumoto Jin
    Department of Applied Chemistry, Faculty of Engineering, University of Miyazaki
  • Higuchi Kazuhiro
    Department of Surgery, Faculty of Medicine, University of Miyazaki
  • Fidya
    Department of Anatomy, Histochemistry and Cell Biology, Faculty of Medicine, University of Miyazaki
  • Shimamawari Koki
    Department of Applied Chemistry, Faculty of Engineering, University of Miyazaki
  • Ishizuka Takumi
    Department of Anatomy, Histochemistry and Cell Biology, Faculty of Medicine, University of Miyazaki
  • Kubota Toshiki
    Department of Anatomy, Histochemistry and Cell Biology, Faculty of Medicine, University of Miyazaki
  • Lkham-Erdene Baljinnyam
    Department of Anatomy, Histochemistry and Cell Biology, Faculty of Medicine, University of Miyazaki
  • Ikenoue Makoto
    Department of Surgery, Faculty of Medicine, University of Miyazaki

Bibliographic Information

Published
2025-04-26
Resource Type
journal article
DOI
  • 10.1267/ahc.25-00002
Publisher
JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY

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<p>Gastric cancer (GC), particularly the undifferentiated type, is frequently associated with peritoneal metastasis, which significantly worsens prognosis due to its resistance to conventional treatments. Photodynamic therapy (PDT) is localized treatment using a photosensitizer (PS) activated by light of a specific wavelength to generate cytotoxic reactive oxygen species that induce cell death. Severe adverse events were reported from clinical trials investigating PDT for peritoneal dissemination conducted until the early 2000s, leaving its safety and clinical effectiveness unestablished. The present study explored whether “non-cytotoxic” PDT using talaporfin sodium (TS) could enhance efficacy of chemotherapeutic agents in undifferentiated GC cell line HGC27. Cell viability was evaluated with MTT assay following TS-PDT, and the synergistic effect between non-cytotoxic TS-PDT and anticancer drug SN-38 was assessed. Changes in expression of drug resistance markers were analyzed through qRT-PCR, Western blotting, and immunocytochemistry. We found that non-cytotoxic TS-PDT enhanced the efficacy of chemotherapy in the undifferentiated GC cell line and reduced the expression of C-X-C chemokine receptor type 4, a key marker associated with GC stem-like properties. These findings highlight the potential of non-cytotoxic TS-PDT as a synergistic treatment approach. We conclude that non-cytotoxic TS-PDT could enhance drug sensitivity and offers a promising therapeutic strategy for GC.</p>

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