{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1390282679028620160.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.2116/bunsekikagaku.50.813"}},{"identifier":{"@type":"COI","@value":"1:CAS:528:DC%2BD38XmtVSr"}},{"identifier":{"@type":"NDL_BIB_ID","@value":"6018494"}},{"identifier":{"@type":"URI","@value":"http://id.ndl.go.jp/bib/6018494"}},{"identifier":{"@type":"URI","@value":"https://ndlsearch.ndl.go.jp/books/R000000004-I6018494"}},{"identifier":{"@type":"URI","@value":"https://dl.ndl.go.jp/pid/10895138"}},{"identifier":{"@type":"NDL_BIB_ID","@value":"10895138"}},{"identifier":{"@type":"NAID","@value":"110002904996"}},{"identifier":{"@type":"URI","@value":"http://id.ndl.go.jp/digimeta/10895138"}},{"identifier":{"@type":"URI","@value":"http://www.jstage.jst.go.jp/article/bunsekikagaku/50/12/50_12_813/_pdf"}},{"identifier":{"@type":"URI","@value":"http://dl.ndl.go.jp/info:ndljp/pid/10895138"}},{"identifier":{"@type":"URI","@value":"https://search.jamas.or.jp/link/ui/2002096887"}}],"dc:title":[{"@language":"en","@value":"Analytical Chemistry for Advanced Technologies. Quantitative determination of K+, NH4+, Na+, Ca2+ and Mg2+ cations in secreted human sweat by capillary zone electrophoresis."},{"@language":"ja","@value":"高度技術を支える分析化学　　キャピラリー電気泳動法によるヒト汗中のナトリウム，カリウム，カルシウム，マグネシウム，アンモニウムイオンの定量"},{"@value":"キャピラリー電気泳動法によるヒト汗中のナトリウム,カリウム,カルシウム,マグネシウム,アンモニウムイオンの定量"},{"@language":"ja-Kana","@value":"キャピラリー デンキ エイドウホウ ニ ヨル ヒト アセ チュウ ノ ナトリウム カリウム カルシウム マグネシウム アンモニウムイオン ノ テイリョウ"},{"@value":"キャピラリー電気泳動法によるヒト汗中のナトリウム, カリウム, カルシウム, マグネシウム, アンモニウムイオンの定量(<特集>高度技術を支える分析化学)"}],"dc:language":"ja","description":[{"type":"abstract","notation":[{"@language":"en","@value":"Cations in human insensible perspiration were analyzed by capillary zone electrophoresis. A simple sampling method from the skin surface of the forefinger of a hand was devised. After washing the forefinger with tap water and then distilled water for 10 s, 40 μl of a 10% ethanol aqueous solution kept in a small vial (0.6 ml) was allowed to contact the treated forefinger for 3 minutes. The sweat sample was then analyzed by capillary zone electrophoresis. A mixture of 5.0 mmol l<sup>-1</sup> copper (II) acetate, 10 mmol l<sup>-1</sup> ethylendiamine and 1.0 mmol l<sup>-1</sup> 18-crown-6-ether at pH 6.5 was selected as the optimum medium for capillary zone electrophoresis. Six cations {Rb<sup>+</sup>(I.S.), K<sup>+</sup>, NH<sub>4</sub><sup>+</sup>, Na<sup>+</sup>, Ca<sup>2+</sup> and Mg<sup>2+</sup>} were completely separated in less than 3 min, and detected with indirect UV detection at 230 nm. Sweat samples were collected from seven volunteers. They were healthy males (<i>n</i> = 4) and females (<i>n</i> = 3), aged 21∼25 years. The mean values of the cation concentrations were K<sup>+</sup> = 39.9 mM, Na<sup>+</sup> = 54.0 mM, Ca<sup>2+</sup> = 5.49 mM, Mg<sup>2+</sup> = 1.67 mM, NH<sub>4</sub><sup>+</sup> = 5.13 mM. It shoud be noted that the concentration of K<sup>+</sup> in secreted sweat is much higher than that in serum."},{"@language":"ja","@value":"簡易的かつ迅速なサンプリング方法を用いて汗を採取し, 汗中に含まれるイオン類を分離・定量した. 主に不感性発汗を10%エタノール水溶液40μlを汗の試料の捕集液として入れた小さなバイアルを手掌部人差し指皮膚表面上で接触させ, 汗を3分間サンプリングした. 次いでキャピラリー電気泳動法によりヒト汗中の陽イオンの分離・定量を行った. 汗中のカリウム, ナトリウム, カルシウム, マグネシウム, アンモニウムイオンを同時に一斉分離することを達成できた. 22～25歳の健常人被験者7人の汗を測定したところ, 各陽イオンの平均値は, K<sup>+</sup>=39.9, Na<sup>+</sup>=54.0, Ca<sup>2+</sup>=5.49, Mg<sup>2+</sup>=1.67, NH<sub>4</sub><sup>+</sup>=5.13mMとなった. 本報告での汗中のカリウムイオン濃度は, 今までの報告値よりも高濃度となり, また血清中よりもかなり濃度が高くなり, 従来にない知見を得た. また, 汗中アンモニア濃度は血清中よりも100倍以上の高濃度となった."}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1410282679028620160","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000004505531"}],"foaf:name":[{"@language":"en","@value":"TERASAWA Junko"},{"@language":"ja","@value":"寺澤 純子"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Applied Chemistry, Nagoya Institute of Technology"},{"@language":"ja","@value":"名古屋工業大学応用化学科"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282679028620162","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000004505532"}],"foaf:name":[{"@language":"en","@value":"MITSUYA Kotaro"},{"@language":"ja","@value":"三矢 光太郎"}],"jpcoar:affiliationName":[{"@language":"ja","@value":"名古屋工業大学応用化学科"},{"@language":"en","@value":"Department of Applied Chemistry, Nagoya Institute of 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Takao"},{"@language":"ja","@value":"津田 孝雄"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Applied Chemistry, Nagoya Institute of Technology"},{"@language":"ja","@value":"名古屋工業大学応用化学科"}]},{"@id":"https://cir.nii.ac.jp/crid/1420001326219160320","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"30252175"},{"@type":"NRID","@value":"1000030252175"},{"@type":"NRID","@value":"9000239627950"},{"@type":"NRID","@value":"9000239866623"},{"@type":"NRID","@value":"9000415156377"},{"@type":"NRID","@value":"9000239627752"},{"@type":"NRID","@value":"9000239627841"},{"@type":"NRID","@value":"9000239627730"},{"@type":"NRID","@value":"9000239627623"},{"@type":"NRID","@value":"9000239627722"},{"@type":"NRID","@value":"9000239627850"},{"@type":"NRID","@value":"9000237784885"},{"@type":"NRID","@value":"9000239627900"},{"@type":"NRID","@value":"9000410469668"},{"@type":"NRID","@value":"9000239627919"},{"@type":"NRID","@value":"9000239627796"},{"@type":"NRID","@value":"9000239627619"},{"@type":"NRID","@value":"9000401514776"},{"@type":"NRID","@value":"9000239627578"},{"@type":"NRID","@value":"9000239627656"},{"@type":"NRID","@value":"9000239627945"},{"@type":"NRID","@value":"9000239627585"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0043585"}],"foaf:name":[{"@language":"en","@value":"ISHII Akira"},{"@language":"ja","@value":"石井 晃"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Legal Medicine and Bioethics, Post Graduate School of Medicine Nagoya University"},{"@language":"ja","@value":"名古屋大学医学部法医学教室"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"05251931"},{"@type":"NDL_BIB_ID","@value":"000000021279"},{"@type":"ISSN","@value":"05251931"},{"@type":"LISSN","@value":"05251931"},{"@type":"PISSN","@value":"http://id.crossref.org/issn/05251931"},{"@type":"NCID","@value":"AN00222633"}],"prism:publicationName":[{"@language":"ja","@value":"分析化学"},{"@language":"en","@value":"BUNSEKI KAGAKU"},{"@language":"en","@value":"BUNSEKI KAGAKU"},{"@language":"en","@value":"Bunseki kagaku"},{"@language":"ja","@value":"分析化学"}],"dc:publisher":[{"@language":"en","@value":"The Japan Society for Analytical Chemistry"},{"@language":"ja","@value":"公益社団法人 日本分析化学会"}],"prism:publicationDate":"2001","prism:volume":"50","prism:number":"12","prism:startingPage":"813","prism:endingPage":"817"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","url":[{"@id":"http://id.ndl.go.jp/bib/6018494"},{"@id":"https://ndlsearch.ndl.go.jp/books/R000000004-I6018494"},{"@id":"https://dl.ndl.go.jp/pid/10895138"},{"@id":"http://id.ndl.go.jp/digimeta/10895138"},{"@id":"http://www.jstage.jst.go.jp/article/bunsekikagaku/50/12/50_12_813/_pdf"},{"@id":"http://dl.ndl.go.jp/info:ndljp/pid/10895138"},{"@id":"https://search.jamas.or.jp/link/ui/2002096887"}],"availableAt":"2001","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=capillary%20zone%20electrophoresis","dc:title":"capillary zone electrophoresis"},{"@id":"https://cir.nii.ac.jp/all?q=cation","dc:title":"cation"},{"@id":"https://cir.nii.ac.jp/all?q=sweat","dc:title":"sweat"},{"@id":"https://cir.nii.ac.jp/all?q=potassium%20ion","dc:title":"potassium 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sweat"}]},{"@id":"https://cir.nii.ac.jp/crid/1363388844913612800","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Studies on Sweat Losses of Nutrients I. 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