Sphingomyelins in Four Ascidians, Ciona intestinalis, Halocynthia roretzi, Halocynthia aurantium, and Styela clava

DOI Web Site Web Site PubMed 被引用文献2件 参考文献10件 オープンアクセス
  • Ito Masahiro
    Department of Bioinformatics, Institute of Science and Engineering, Ritsumeikan University
  • Yokoi Kazuhito
    Department of Bioinformatics, Institute of Science and Engineering, Ritsumeikan University
  • Inoue Takashi
    Department of Bioinformatics, Institute of Science and Engineering, Ritsumeikan University
  • Asano Shogo
    Department of Bioinformatics, Institute of Science and Engineering, Ritsumeikan University Graduate School of Frontier Biosciences, Graduate School of Medicine, Osaka University
  • Hatano Rei
    Department of Bioinformatics, Institute of Science and Engineering, Ritsumeikan University
  • Shinohara Ryota
    Department of Bioinformatics, Institute of Science and Engineering, Ritsumeikan University Department of Pharmacology, Kyoto University Faculty of Medicine
  • Itonori Saki
    Department of Chemistry, Faculty of Liberal Arts and Education, Shiga University
  • Sugita Mutsumi
    Department of Chemistry, Faculty of Liberal Arts and Education, Shiga University

書誌事項

公開日
2009
資源種別
journal article
DOI
  • 10.5650/jos.58.473
公開者
公益社団法人 日本油化学会

この論文をさがす

説明

Sphingomyelin is rarely found in lower animals, while sphingophospholipid is a characteristic of higher animals. In this study, sphingomyelin was first isolated and characterized from ascidian Ciona intestinalis. Ascidian sphingomyelin was prepared using ion exchange (QAE-Sphadex-A25) and silicic acid (Florisil and Iatrobeads) column chromatographies. The chemical structure was characterized by fatty acid analysis, sphingoid analysis, hydrogen fluoride degradation, acid hydrolysis, enzymatic hydrolysis, infrared analysis, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The ceramide moieties of C. intestinalis sphingomyelin consisted primarily of C16:0, C18:0, and C18:1 fatty acids and d18:2 sphingadiene. Furthermore, sphingomyelins were isolated and characterized from 3 other ascidians, Halocynthia roretzi, Halocynthia aurantium, and Styela clava using the same methods. Comparative analysis of the sphingomyelin structures in 4 ascidian species-C. intestinalis (Enterogona) and H. roretzi, H. aurantium, and S. clava (Pleurogona)-revealed that the major fatty acid composition of the ceramides was similar, and that they differed in minor components.

収録刊行物

被引用文献 (2)*注記

もっと見る

参考文献 (10)*注記

もっと見る

関連プロジェクト

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ