Medium optimization by orthogonal array and response surface methodology for cholesterol oxidase production by Streptomyces lavendulae NCIM 2499
-
- K. Chauhan Awadesh
- Food Engineering and Technology Department, Institute of Chemical Technology, University of Mumbai
-
- A. Survase Shrikan
- Food Engineering and Technology Department, Institute of Chemical Technology, University of Mumbai
-
- Kishenkumar Jyoti
- Food Engineering and Technology Department, Institute of Chemical Technology, University of Mumbai
-
- S. Annapure Uday
- Food Engineering and Technology Department, Institute of Chemical Technology, University of Mumbai
説明
This paper deals with the optimization of culture conditions for the production of cholesterol oxidase (COD) by Streptomyces lavendulae NCIM 2499 using the one-factor-at-a-time method, orthogonal array method and response surface methodology (RSM) approaches. The one-factor-at-a-time method was adopted to investigate the effects of medium components (i.e. carbon and nitrogen) and environmental factors (i.e. initial pH) on biomass growth and COD production. Subsequently, an L12 orthogonal matrix was used to evaluate the significance of glycerol, soyabean meal, malt extract, K2HPO4, MgSO4 and NaCl. The effects of media components were ranked according to their effects on the production of COD as malt extract > soyabean meal > K2HPO4 > NaCl > MgSO4 > glycerol. The subsequent optimization of the four most significant factors viz. malt extract, soyabean meal, K2HPO4 and NaCl, was carried out by employing a central composite rotatable design (CCRD) of RSM. There was a 2.48-fold increase in productivity of COD as compared to the unoptimized media by using these statistical approaches.
収録刊行物
-
- The Journal of General and Applied Microbiology
-
The Journal of General and Applied Microbiology 55 (3), 171-180, 2009
公益財団法人 応用微生物学・分子細胞生物学研究奨励会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390282679121966592
-
- NII論文ID
- 130004802139
-
- COI
- 1:CAS:528:DC%2BD1MXot1amu7Y%3D
-
- ISSN
- 13498037
- 00221260
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- Crossref
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可