{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1390282679158352000.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1143/jpsj.66.975"}},{"identifier":{"@type":"BIBCODE","@value":"1997JPSJ...66..975E"}},{"identifier":{"@type":"NDL_BIB_ID","@value":"4209375"}},{"identifier":{"@type":"URI","@value":"http://id.ndl.go.jp/bib/4209375"}},{"identifier":{"@type":"URI","@value":"https://ndlsearch.ndl.go.jp/books/R000000004-I4209375"}},{"identifier":{"@type":"NAID","@value":"210000100707"}},{"identifier":{"@type":"NAID","@value":"110001970345"}},{"identifier":{"@type":"NAID","@value":"130004536074"}}],"dc:title":[{"@language":"en","@value":"Light-Induced Denaturation of Bacteriorhodopsin Just above Melting Point of Two-Dimensional Crystal."},{"@value":"Light-Induced Denaturation of Bacteriorhodopsin Just abobe Melting Point of Two-Dimensional Crystal"},{"@language":"ja-Kana","@value":"Light-Induced Denaturation of Bacterior"}],"dc:language":"en","description":[{"type":"abstract","notation":[{"@language":"en","@value":"The denaturation kinetics of bacteriorhodopsin induced by light irradiation was measured in the temperature range between 80 and 86°C, which was just above the melting point of the two-dimensional crystal composed of bacteriorhodopsin molecules in the purple membrane. Bacteriorhodopsin was stable in the dark but gradually denatured under the irradiation of visible light longer than 520, nm. Analyzing the time course of the light-induced denaturation phenomenon, the kinetic constant of the denaturation from a photointermediate state was estimated. The temperature dependence of the kinetic constant lead to the effective activation energy of about 41, kcal/mol. The stability of a photointermediate state was discussed on the basis of the light-induced denaturation behavior."}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1410282679158352000","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000401878499"},{"@type":"NRID","@value":"9000003322373"},{"@type":"NRID","@value":"9000258189672"}],"foaf:name":[{"@language":"en","@value":"Itoh Hiroyasu"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Tsukuba Research Laboratory, Hamamatsu Photonics K.K., Tokodai 5–9–2, Ibaraki 300–26"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282679158352001","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000401878498"},{"@type":"NRID","@value":"9000003322370"},{"@type":"NRID","@value":"9000258189671"}],"foaf:name":[{"@language":"en","@value":"Etoh Akinori"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Faculty of Technology, Department of Biotechnology, Tokyo University of Agriculture and Technology, Nakacho 2–24–16, Koganei, Tokyo 184"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282679158352002","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000401878500"},{"@type":"NRID","@value":"9000258189673"}],"foaf:name":[{"@language":"en","@value":"Mitaku Shigeki"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Faculty of Technology, Department of Biotechnology, Tokyo University of Agriculture and Technology, Nakacho 2–24–16, Koganei, Tokyo 184"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00319015"},{"@type":"LISSN","@value":"00319015"},{"@type":"EISSN","@value":"13474073"},{"@type":"NDL_BIB_ID","@value":"000000118709"},{"@type":"ISSN","@value":"00319015"},{"@type":"NCID","@value":"AA00704814"}],"prism:publicationName":[{"@language":"en","@value":"Journal of the Physical Society of Japan"},{"@language":"ja","@value":"Ｊｏｕｒｎａｌ　ｏｆ　ｔｈｅ　Ｐｈｙｓｉｃａｌ　Ｓｏｃｉｅｔｙ　ｏｆ　Ｊａｐａｎ"},{"@language":"en","@value":"JOURNAL OF THE PHYSICAL SOCIETY OF JAPAN"},{"@language":"en","@value":"JPSJ"},{"@language":"ja","@value":"ＪＰＳＪ"}],"dc:publisher":[{"@language":"en","@value":"THE PHYSICAL SOCIETY OF JAPAN"},{"@language":"ja","@value":"一般社団法人 日本物理学会"}],"prism:publicationDate":"1997","prism:volume":"66","prism:number":"4","prism:startingPage":"975","prism:endingPage":"978"},"reviewed":"false","url":[{"@id":"http://id.ndl.go.jp/bib/4209375"},{"@id":"https://ndlsearch.ndl.go.jp/books/R000000004-I4209375"}],"availableAt":"1997","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=denaturation","dc:title":"denaturation"},{"@id":"https://cir.nii.ac.jp/all?q=bacteriorhodopsin","dc:title":"bacteriorhodopsin"},{"@id":"https://cir.nii.ac.jp/all?q=photointermediate%20state","dc:title":"photointermediate state"},{"@id":"https://cir.nii.ac.jp/all?q=activation%20energy","dc:title":"activation energy"},{"@id":"https://cir.nii.ac.jp/all?q=kinetics","dc:title":"kinetics"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050291768265904512","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Pore Size Distributions Related with Spontaneous Purple Membrane Stacking in Porous Hydrogels"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004233920967168","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Spontaneous stacking of purple membranes during immobilization with physical cross-linked poly(vinyl alcohol) hydrogel with retaining native-like functionality of bacteriorhodopsin"}]},{"@id":"https://cir.nii.ac.jp/crid/1360009142920136704","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Influence of osmolytes and ionic liquids on the 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