Microchip electrophoresis system for the evaluation of RNA quality

DOI 8 References
  • Takeda Mayu
    Division of Healthcare Economics and Quality Management, Tohoku Fukushi University 3Department of Biomedical Informatics, Division of Health Sciences, Osaka University Graduate School of Medicine
  • Funato Tadao
    Division of Healthcare Economics and Quality Management, Tohoku Fukushi University
  • Fujimaki Schinichi
    Division of Molecular Diagnostics, Tohoku University School of Medicine
  • Iwatani Yoshinori
    3Department of Biomedical Informatics, Division of Health Sciences, Osaka University Graduate School of Medicine

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  • オンチップ電気泳動システムによるＲＮＡの品質評価の検討

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RNA degradation is a critical problem in all experimental settings and for clinical samples for the analysis of gene expression. The sufficient integrity of starting RNA is the essential factor that determines the accuracy of mRNA measurements such as quantitative real-time reverse-transcription PCR (RT-qPCR) and microarray experiments. We evaluated RNA yield and quality of RNA samples and obtained RNA Integrity Number (RIN) with a microchip electrophoresis system. All samples of frozen RNA and RNA extracted from cultured cells revealed RIN of more than 9.8, and showed a discrete, high-molecular-weight band on agarose gel electrophoresis. RIN was not affected by repeated freezing and thawing. These results suggested that the RIN could be a suitable indicator for the integrity of RNA during storage.<br>

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