Basic and clinieal studies on isozyme of alkaline phosphatase

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  • アルカリフォスファターゼアイソザイムの基礎的および臨床的研究

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Alkaline phosphatase isozyme of serum and tissue extracts were studied by electrophoresis using polyacrylamide as a supporting medium. Location of the enzyme activity were determined by diazo-coupling staining method.<br>Four separate zones of the enzyme activity were found in various tissue extracts of normal rabbit and human origin, and in normal and pathological human sera.<br>Sera from patients with liver or bone disease showed specific patterns of alkaline phosphatase activity.<br>Sera from patients with obstruction of hepatic or common biliary duct and the bone extracts of the same patient showed analogous isozyme patterns.

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