{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1390282679303572736.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.5511/plantbiotechnology.14.1210b"}},{"identifier":{"@type":"NDL_BIB_ID","@value":"026292486"}},{"identifier":{"@type":"URI","@value":"http://id.ndl.go.jp/bib/026292486"}},{"identifier":{"@type":"URI","@value":"https://ndlsearch.ndl.go.jp/books/R000000004-I026292486"}},{"identifier":{"@type":"URI","@value":"https://dl.ndl.go.jp/pid/11000416"}},{"identifier":{"@type":"NDL_BIB_ID","@value":"11000416"}},{"identifier":{"@type":"URI","@value":"http://id.ndl.go.jp/digimeta/11000416"}},{"identifier":{"@type":"NAID","@value":"130005061602"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@language":"en","@value":"Double-stranded DNA introduction into intact plants using peptide–DNA complexes"},{"@value":"Double-stranded DNA introduction into intact plants using peptide&#x2013;DNA complexes"}],"dc:language":"en","description":[{"type":"abstract","notation":[{"@language":"en","@value":"Introducing exogenous genes into plant cells is an essential technique in many fields in plant science and biotechnology. Despite their huge advantages, disadvantages of current transfection methods include the requirement of expensive equipment, risk of gene damage, low transformation efficiency, transgene size limitations, and limitations of applicable plant types. Recently developed peptide-based gene carriers can deliver plasmid and double-stranded RNA. However, the delivery of double-stranded DNA (dsDNA), specifically PCR products, has not been studied. As dsDNA is handled in several plant science labs, peptide-based gene carriers are expected to be applicable to dsDNA in addition to plasmid DNA and double-stranded RNA. Here, we demonstrate dsDNA introduction into intact <i>Nicotiana benthamiana</i> leaves by using an ionic complex of a fusion peptide comprising (KH)<sub>9</sub> and Bp100 with dsDNA encoding <i>Renilla</i> luciferase as a reporter gene. The buffer condition for the complex preparation and infiltration significantly affected the transfection efficiency; this is because the structure of the complex in various protonated conditions contributed to the transfection efficiency. Structures of the complex and peptide are key factors for improving the peptide-based gene delivery system for plants."}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1410282679303572736","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000286624397"}],"foaf:name":[{"@language":"en","@value":"Lakshmanan Manoj"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Enzyme Research Team, Biomass Engineering Program Cooperative Division, RIKEN Center for Sustainable Resource Science"},{"@language":"en","@value":"Ecobiomaterial Research Laboratory, School of Biological Science, Universiti Sains Malaysia"}]},{"@id":"https://cir.nii.ac.jp/crid/1420001326213721728","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"80342872"},{"@type":"NRID","@value":"1000080342872"},{"@type":"NRID","@value":"9000391935238"},{"@type":"NRID","@value":"9000391923377"},{"@type":"NRID","@value":"9000265562065"},{"@type":"NRID","@value":"9000018277433"},{"@type":"NRID","@value":"9000391946692"},{"@type":"NRID","@value":"9000391922014"},{"@type":"NRID","@value":"9000391943674"},{"@type":"NRID","@value":"9000391972035"},{"@type":"NRID","@value":"9000391917467"},{"@type":"NRID","@value":"9000000190751"},{"@type":"NRID","@value":"9000375908249"},{"@type":"NRID","@value":"9000391955471"},{"@type":"NRID","@value":"9000391935230"},{"@type":"NRID","@value":"9000391935220"},{"@type":"NRID","@value":"9000391957540"},{"@type":"NRID","@value":"9000019137825"},{"@type":"NRID","@value":"9000001958064"},{"@type":"NRID","@value":"9000391946263"},{"@type":"NRID","@value":"9000391940793"},{"@type":"NRID","@value":"9000391943693"},{"@type":"NRID","@value":"9000391948618"},{"@type":"NRID","@value":"9000391952782"},{"@type":"NRID","@value":"9000391922026"},{"@type":"NRID","@value":"9000391953132"},{"@type":"NRID","@value":"9000391954266"},{"@type":"NRID","@value":"9000391959017"},{"@type":"NRID","@value":"9000391934621"},{"@type":"NRID","@value":"9000391954507"},{"@type":"NRID","@value":"9000391928499"},{"@type":"NRID","@value":"9000391955592"},{"@type":"NRID","@value":"9000402256598"},{"@type":"NRID","@value":"9000006201971"},{"@type":"NRID","@value":"9000391962510"},{"@type":"NRID","@value":"9000391958380"},{"@type":"NRID","@value":"9000391933618"},{"@type":"NRID","@value":"9000391938487"},{"@type":"NRID","@value":"9000391937609"},{"@type":"NRID","@value":"9000391936188"},{"@type":"NRID","@value":"9000391961459"},{"@type":"NRID","@value":"9000411979756"},{"@type":"NRID","@value":"9000391951410"},{"@type":"NRID","@value":"9000347296549"},{"@type":"NRID","@value":"9000286624398"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/zumi-takasaki"}],"foaf:name":[{"@language":"en","@value":"Yoshizumi Takeshi"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Institute for Advanced Biosciences, Keio University"}]},{"@id":"https://cir.nii.ac.jp/crid/1420564276158577664","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"00455213"},{"@type":"NRID","@value":"1000000455213"},{"@type":"NRID","@value":"9000002146316"},{"@type":"NRID","@value":"9000345217684"},{"@type":"NRID","@value":"9000403148598"},{"@type":"NRID","@value":"9000309167253"},{"@type":"NRID","@value":"9000402079316"},{"@type":"NRID","@value":"9000359881961"},{"@type":"NRID","@value":"9000404636021"},{"@type":"NRID","@value":"9000002146120"},{"@type":"NRID","@value":"9000286624432"},{"@type":"NRID","@value":"9000411998960"},{"@type":"NRID","@value":"9000412405668"},{"@type":"NRID","@value":"9000398302034"},{"@type":"NRID","@value":"9000414819994"},{"@type":"NRID","@value":"9000398985739"},{"@type":"NRID","@value":"9000413505894"},{"@type":"NRID","@value":"9000405759649"},{"@type":"NRID","@value":"9000284274319"},{"@type":"NRID","@value":"9000018661030"},{"@type":"NRID","@value":"9000014187468"},{"@type":"NRID","@value":"9000396127890"},{"@type":"NRID","@value":"9000242694275"},{"@type":"NRID","@value":"9000403261175"},{"@type":"NRID","@value":"9000411979758"},{"@type":"NRID","@value":"9000312722507"},{"@type":"NRID","@value":"9000286624400"},{"@type":"NRID","@value":"9000399777826"},{"@type":"NRID","@value":"9000399567752"},{"@type":"NRID","@value":"9000370732915"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/yutakakodama"}],"foaf:name":[{"@language":"en","@value":"Kodama Yutaka"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Center for Bioscience Research and Education, Utsunomiya University"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282679303572738","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000286624401"}],"foaf:name":[{"@language":"en","@value":"Numata Keiji"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Enzyme Research Team, Biomass Engineering Program Cooperative Division, RIKEN Center for Sustainable Resource Science"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282679303572740","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000286624399"}],"foaf:name":[{"@language":"en","@value":"Sudesh Kumar"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Ecobiomaterial Research Laboratory, School of Biological Science, Universiti Sains Malaysia"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"13424580"},{"@type":"LISSN","@value":"13424580"},{"@type":"EISSN","@value":"13476114"},{"@type":"CODEN","@value":"PLBIF6"},{"@type":"NDL_BIB_ID","@value":"000000157745"},{"@type":"ISSN","@value":"13424580"},{"@type":"ISSN","@value":"13476114"},{"@type":"NCID","@value":"AA11250821"}],"prism:publicationName":[{"@language":"en","@value":"Plant Biotechnology"},{"@language":"ja","@value":"Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ"},{"@language":"en","@value":"Plant Biotechnology"},{"@language":"ja","@value":"Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ"}],"dc:publisher":[{"@language":"en","@value":"Japanese Society for Plant Biotechnology"},{"@language":"ja","@value":"日本植物バイオテクノロジー学会"}],"prism:publicationDate":"2015","prism:volume":"32","prism:number":"1","prism:startingPage":"39","prism:endingPage":"45"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","url":[{"@id":"http://id.ndl.go.jp/bib/026292486"},{"@id":"https://ndlsearch.ndl.go.jp/books/R000000004-I026292486"},{"@id":"https://dl.ndl.go.jp/pid/11000416"},{"@id":"http://id.ndl.go.jp/digimeta/11000416"}],"availableAt":"2015","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Double-stranded%20DNA","dc:title":"Double-stranded DNA"},{"@id":"https://cir.nii.ac.jp/all?q=gene%20carrier%20peptide","dc:title":"gene carrier peptide"},{"@id":"https://cir.nii.ac.jp/all?q=gene%20delivery","dc:title":"gene delivery"}],"project":[{"@id":"https://cir.nii.ac.jp/crid/1040000782317604352","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"26840088"},{"@type":"JGN","@value":"JP26840088"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26840088/"}],"notation":[{"@language":"ja","@value":"ゼニゴケＴ－ＤＮＡ挿入変異体集団を用いたオルガネラ低温定位運動の制御遺伝子探索"},{"@language":"en","@value":"Screening for genes involved in the cold-induced organelle relocation using a T-DNA insertion library of the liverwort Marchantia polymorpha"}]}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050016880901693568","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Plant Mitochondrial-Targeted Gene Delivery by Peptide/DNA Micelles Quantitatively Surface-Modified with Mitochondrial Targeting and Membrane-Penetrating Peptides"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285708189375232","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Quantized Folding of Plasmid DNA Condensed with Block Catiomer into Characteristic Rod Structures Promoting Transgene Efficacy"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285710786376064","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Local gene silencing in plants via synthetic ds<scp>RNA</scp> and carrier peptide"}]},{"@id":"https://cir.nii.ac.jp/crid/1360290617744394624","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Endosome-escaping micelle complexes dually equipped with cell-penetrating and endosome-disrupting peptides for efficient DNA delivery into intact plants"}]},{"@id":"https://cir.nii.ac.jp/crid/1360292618503651840","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Transfection and expression of plasmid DNA in plant cells by an arginine‐rich intracellular delivery peptide without protoplast preparation"}]},{"@id":"https://cir.nii.ac.jp/crid/1360292619719120768","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Bioengineered silk protein-based gene delivery systems"}]},{"@id":"https://cir.nii.ac.jp/crid/1360292621510414464","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Adsorption and Hydrolysis Reactions of Poly(hydroxybutyric acid) Depolymerases Secreted from <i>Ralstonia</i> <i>pickettii</i> T1 and <i>Penicillium </i><i>funiculosum</i> onto Poly[(<i>R</i>)-3-hydroxybutyric acid]"}]},{"@id":"https://cir.nii.ac.jp/crid/1360298754813829248","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Particle bombardment-assisted peptide-mediated gene transfer for highly efficient transient assay"}]},{"@id":"https://cir.nii.ac.jp/crid/1360565164562467072","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Silk‐Based Nanocomplexes with Tumor‐Homing Peptides for Tumor‐Specific Gene Delivery"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567183118526464","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Rapid and Efficient Gene Delivery into Plant Cells Using Designed Peptide Carriers"}]},{"@id":"https://cir.nii.ac.jp/crid/1360574095100081152","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Co-polymer of histidine and lysine markedly enhances transfection efficiency of 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bacteria"}]},{"@id":"https://cir.nii.ac.jp/crid/1360855569776764416","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"<i>Arabidopsis</i>\n                    Nitric Oxide Synthase1 Is Targeted to Mitochondria and Protects against Oxidative Damage and Dark-Induced Senescence"}]},{"@id":"https://cir.nii.ac.jp/crid/1360855570115459968","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Silica breaks through in plants"}]},{"@id":"https://cir.nii.ac.jp/crid/1360861291387378048","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Functional peptide-mediated plastid transformation in tobacco, rice, and kenaf"}]},{"@id":"https://cir.nii.ac.jp/crid/1361131417891076224","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Dual Peptide-Based Gene Delivery System for the Efficient Transfection of Plant Callus Cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1361418520073002496","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Spider Silk-Based Gene Carriers for Tumor Cell-Specific Delivery"}]},{"@id":"https://cir.nii.ac.jp/crid/1361699993775843584","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Silk-Based Gene Carriers with Cell Membrane Destabilizing Peptides"}]},{"@id":"https://cir.nii.ac.jp/crid/1361981469976141952","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Chloroplast Transformation in\n            <i>Chlamydomonas</i>\n            with High Velocity Microprojectiles"}]},{"@id":"https://cir.nii.ac.jp/crid/1362262944192573824","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Gene delivery mediated by recombinant silk proteins containing cationic and cell binding motifs"}]},{"@id":"https://cir.nii.ac.jp/crid/1362544419834610176","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Study of uptake of cell penetrating peptides and their cargoes in permeabilized wheat immature embryos"}]},{"@id":"https://cir.nii.ac.jp/crid/1362825894298348416","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Solid phase peptide synthesis utilizing 9‐fluorenylmethoxycarbonyl amino acids"}]},{"@id":"https://cir.nii.ac.jp/crid/1363107371253976448","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Cationic oligopeptide‐mediated delivery of dsRNA for post‐transcriptional gene silencing in plant cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1363388844522760320","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Adsorption of Biopolyester Depolymerase on Silicon Wafer and Poly[(<i>R</i>)‐3‐hydroxybutyric acid] Single Crystal Revealed by Real‐Time AFM"}]},{"@id":"https://cir.nii.ac.jp/crid/1363388845474708992","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Silk-based delivery systems of bioactive molecules"}]},{"@id":"https://cir.nii.ac.jp/crid/1363670320670217344","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Agrobacterium tumefaciens transfers extremely long T-DNAs by a unidirectional mechanism"}]},{"@id":"https://cir.nii.ac.jp/crid/1363670320807496064","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Enzymatic Degradation Processes of Poly[(<i>R</i>)-3-hydroxybutyric acid] and Poly[(<i>R</i>)-3-hydroxybutyric acid-<i>c</i><i>o</i>-(<i>R</i>)-3-hydroxyvaleric acid] Single Crystals Revealed by Atomic Force Microscopy:  Effects of Molecular Weight and Second-Monomer Composition on Erosion Rates"}]},{"@id":"https://cir.nii.ac.jp/crid/1364233268340329600","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Gene transfer to plants by diverse species of bacteria"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001288143737216","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"A centrifugation-assisted peptide-mediated gene transfer method for high-throughput analyses"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679303460096","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Direct introduction of neomycin phosphotransferase II protein into apple leaves to confer kanamycin 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