{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1390282679303628160.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.5511/plantbiotechnology.12.0406a"}},{"identifier":{"@type":"COI","@value":"1:CAS:528:DC%2BC38Xht1Chtb3M"}},{"identifier":{"@type":"DOI","@value":"10.5511/plantbiotechnology.12.406a"}},{"identifier":{"@type":"NDL_BIB_ID","@value":"023784456"}},{"identifier":{"@type":"URI","@value":"http://id.ndl.go.jp/bib/023784456"}},{"identifier":{"@type":"URI","@value":"https://ndlsearch.ndl.go.jp/books/R000000004-I023784456"}},{"identifier":{"@type":"URI","@value":"https://dl.ndl.go.jp/pid/10205083"}},{"identifier":{"@type":"NAID","@value":"10030804744"}},{"identifier":{"@type":"NAID","@value":"130001883169"}}],"dc:title":[{"@language":"en","@value":"Establishment of a novel system to elucidate the mechanisms underlying light-induced ripening of strawberry fruit with an <i>Agrobacterium</i>-mediated RNAi technique"},{"@value":"Establishment of a novel system to elucidate the mechanisms underlying light-induced ripening of strawberry fruit with an Agrobacterium-mediated RNAi technique"},{"@value":"10.5511/plantbiotechnology.12.406a"}],"dc:language":"en","description":[{"type":"abstract","notation":[{"@language":"en","@value":"Traditional methods used to study strawberry ripening-related gene function are time-consuming, and require at least 15 months from initiating the transformation experiment until the first ripe fruits are available for analysis. To accelerate data acquisition during gene function studies, we explored a transient assay method that employs an <i>Agrobacterium</i>-mediated RNAi (<i>A</i>mRNAi) technique in post-harvest strawberry fruit, <i>Fragaria</i>×<i>ananassa</i> (<i>Fa</i>) cv. Sachinoka, a Japanese cultivar. Our results showed that artificial white light induced strong expression of <i>Fa′chalcone synthase</i> (<i>Fa′CHS</i>), <i>Fa′chalcone isomerase</i> (<i>Fa′CHI</i>), and <i>Fa′flavonoid 3′-hydroxylase</i> orthologues (<i>Fa′F3′H</i>) in post-harvest fruit. <i>Fa′CHS</i> and <i>Fa′F3′H</i> function was subsequently examined by performing <i>A</i>mRNAi with post-harvest fruit. Although reduction of light-induced <i>Fa′F3′H</i> expression by <i>A</i>mRNAi resulted in no significant change in anthocyanin content, reduction of <i>Fa′CHS</i> significantly decreased anthocyanin levels, and up-regulated <i>Fa′F3′H</i> levels. Our results are consistent with previous data indicating that while <i>CHS</i> is required for anthocyanin accumulation during late stage strawberry fruit maturation, <i>Fa′F3′H</i> is not required. The novel system described here enabled gene function data to be available within 10 days of initiating the incubation period following infiltration. Therefore, we conclude our system is a valuable tool to elucidate the molecular mechanisms underlying light-induced ripening of strawberry fruit."}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1410282679303628165","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000000981425"},{"@type":"NRID","@value":"9000021955023"}],"foaf:name":[{"@language":"en","@value":"Miyawaki Katsuyuki"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Life Systems, Institute of Technology and Science, The University of Tokushima"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282679303628166","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000019194324"},{"@type":"NRID","@value":"9000021955026"}],"foaf:name":[{"@language":"en","@value":"Hamaoka Hirokazu"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Life Systems, Institute of Technology and Science, The University of Tokushima"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282679303628161","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000019194322"},{"@type":"NRID","@value":"9000021955024"}],"foaf:name":[{"@language":"en","@value":"Fukuoka Sachi"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Life Systems, Institute of Technology and Science, The University of Tokushima"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282679303628167","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000019194323"},{"@type":"NRID","@value":"9000021955025"}],"foaf:name":[{"@language":"en","@value":"Kadomura Yasuko"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Nutrition, Faculty of Medicine, The University of Tokushima"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282679303628164","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000006338809"},{"@type":"NRID","@value":"9000021955028"}],"foaf:name":[{"@language":"en","@value":"Mito Taro"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Life Systems, Institute of Technology and Science, The University of Tokushima"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282679303628160","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000019194325"},{"@type":"NRID","@value":"9000021955032"}],"foaf:name":[{"@language":"en","@value":"Schwab Wilfried"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Biotechnology of Natural Products, Technical University München"}]},{"@id":"https://cir.nii.ac.jp/crid/1420845751157089024","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"00253229"},{"@type":"NRID","@value":"1000000253229"},{"@type":"NRID","@value":"9000000848598"},{"@type":"NRID","@value":"9000414332199"},{"@type":"NRID","@value":"9000283280856"},{"@type":"NRID","@value":"9000000191092"},{"@type":"NRID","@value":"9000414942015"},{"@type":"NRID","@value":"9000258062146"},{"@type":"NRID","@value":"9000387431804"},{"@type":"NRID","@value":"9000401560511"},{"@type":"NRID","@value":"9000001945266"},{"@type":"NRID","@value":"9000414332204"},{"@type":"NRID","@value":"9000322108605"},{"@type":"NRID","@value":"9000021955030"},{"@type":"NRID","@value":"9000410999742"},{"@type":"NRID","@value":"9000404848016"},{"@type":"NRID","@value":"9000409883065"},{"@type":"NRID","@value":"9000398801509"},{"@type":"NRID","@value":"9000364703205"},{"@type":"NRID","@value":"9000242119440"},{"@type":"NRID","@value":"9000409650629"},{"@type":"NRID","@value":"9000315684143"},{"@type":"NRID","@value":"9000398390182"},{"@type":"NRID","@value":"9000410145915"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/ohuchihideyo"}],"foaf:name":[{"@language":"en","@value":"Ohuchi Hideyo"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Life Systems, Institute of Technology and Science, The University of Tokushima"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282679303628162","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000004698482"},{"@type":"NRID","@value":"9000021955034"}],"foaf:name":[{"@language":"en","@value":"Noji Sumihare"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Life Systems, Institute of Technology and Science, The University of Tokushima"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"13424580"},{"@type":"LISSN","@value":"13424580"},{"@type":"EISSN","@value":"13476114"},{"@type":"CODEN","@value":"PLBIF6"},{"@type":"NDL_BIB_ID","@value":"000000157745"},{"@type":"ISSN","@value":"13424580"},{"@type":"PISSN","@value":"08496757"},{"@type":"NCID","@value":"AA11250821"}],"prism:publicationName":[{"@language":"en","@value":"Plant Biotechnology"},{"@language":"ja","@value":"Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ"},{"@language":"en","@value":"Plant Biotechnology"},{"@language":"ja","@value":"Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ"}],"dc:publisher":[{"@language":"en","@value":"Japanese Society for Plant Biotechnology"},{"@language":"ja","@value":"日本植物バイオテクノロジー学会"}],"prism:publicationDate":"2012","prism:volume":"29","prism:number":"3","prism:startingPage":"271","prism:endingPage":"277"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","url":[{"@id":"http://id.ndl.go.jp/bib/023784456"},{"@id":"https://ndlsearch.ndl.go.jp/books/R000000004-I023784456"},{"@id":"https://dl.ndl.go.jp/pid/10205083"}],"availableAt":"2012","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=%3Ci%3EAgrobacterium%3C/i%3E-mediated%20RNAi","dc:title":"<i>Agrobacterium</i>-mediated RNAi"},{"@id":"https://cir.nii.ac.jp/all?q=%3Ci%3Echalcone%20synthase%3C/i%3E","dc:title":"<i>chalcone synthase</i>"},{"@id":"https://cir.nii.ac.jp/all?q=%3Ci%3Eflavonoid%203%E2%80%B2-hydroxylase%3C/i%3E","dc:title":"<i>flavonoid 3′-hydroxylase</i>"},{"@id":"https://cir.nii.ac.jp/all?q=light-induced%20pigmentation","dc:title":"light-induced pigmentation"},{"@id":"https://cir.nii.ac.jp/all?q=strawberry%20fruit","dc:title":"strawberry fruit"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360004231238834304","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Light and abscisic acid independently regulated FaMYB10 in Fragaria × ananassa fruit"}]},{"@id":"https://cir.nii.ac.jp/crid/1360565165304263296","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Phototropin 2 is involved in blue light-induced anthocyanin accumulation in Fragaria x ananassa fruits"}]},{"@id":"https://cir.nii.ac.jp/crid/1360574095198679296","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"RNAi‐induced silencing of gene expression in strawberry fruit (<i>Fragaria</i> × <i>ananassa</i>) by agroinfiltration: a rapid assay for gene function analysis"}]},{"@id":"https://cir.nii.ac.jp/crid/1361137045038862208","@type":"Article","relationType":["references","cites"],"jpcoar:relatedTitle":[{"@value":"Isolation, cloning and expression of a multifunctional O‐methyltransferase capable of forming 2,5‐dimethyl‐4‐methoxy‐3(2H)‐furanone, one of the key aroma compounds in strawberry fruits"}]},{"@id":"https://cir.nii.ac.jp/crid/1361137045777958016","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"An R2R3 MYB transcription factor associated with regulation of the anthocyanin biosynthetic pathway in Rosaceae"}]},{"@id":"https://cir.nii.ac.jp/crid/1361981469134011904","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Gene expression analysis of strawberry achene and receptacle maturation using DNA microarrays"}]},{"@id":"https://cir.nii.ac.jp/crid/1361981470355198464","@type":"Article","relationType":["references","cites"],"jpcoar:relatedTitle":[{"@value":"The strawberry <i>FaMYB1</i> transcription factor suppresses anthocyanin and flavonol accumulation in transgenic tobacco"}]},{"@id":"https://cir.nii.ac.jp/crid/1362825894199483776","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"A simple protocol for transient gene expression in ripe fleshy fruit mediated by Agrobacterium"}]},{"@id":"https://cir.nii.ac.jp/crid/1363388845521316992","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The strawberry transcription factor FaMYB1 inhibits the biosynthesis of proanthocyanidins in Lotus corniculatus leaves"}]},{"@id":"https://cir.nii.ac.jp/crid/1363670320060772864","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Developmental, genetic and environmental factors affect the expression of flavonoid genes, enzymes and metabolites in strawberry fruits*"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001206303302400","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references","cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Color and Anthocyanin Composition of Strawberry Fruit. Changes during Fruit Development and Differences among Cultivars, with Special Reference to the Occurrence of Pelargonidin 3-malonylglucoside."},{"@language":"ja","@value":"イチゴの果色とアントシアニン濃度, 組成の成熟に伴う変化と品種間差異 : 特に Pelargonidin 3-malonylglucoside 生成との関係について"}]},{"@id":"https://cir.nii.ac.jp/crid/1570009751567795200","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"The strawberry transcription factor FaMYB1 inhibits the biosynthesis of proanthocyanidins in Lotus corniculatus leaves"}]},{"@id":"https://cir.nii.ac.jp/crid/1570291225855076352","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"An R2R3 MYB transcription factor associated with regulation of the anthocyanin biosynthetic pathway in Rosaceae"}]},{"@id":"https://cir.nii.ac.jp/crid/1570572701503493376","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Antisense inhibition of a pectate lyase gene supports a role for pectin depolymerization in strawberry fruit softening"}]},{"@id":"https://cir.nii.ac.jp/crid/1570854175808494464","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Two-phase flavonoid formation in developing strawberry (Fragaria×ananassa) fruit"}]},{"@id":"https://cir.nii.ac.jp/crid/1570854175808495488","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"RNAi-induced silencing of gene expression in strawberry fruit (Fragaria×ananassa) by agroinfiltration : a rapid assay for gene function analysis"}]},{"@id":"https://cir.nii.ac.jp/crid/1571135651708919936","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Isolation of a set of ripening-related genes from strawberry : their identification and possible relationship to fruit quality traits"}]},{"@id":"https://cir.nii.ac.jp/crid/1571698601429010304","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Comparison of transcriptional profiles of flavonoid genes and anthocyanin contents during fruit development of two botanical forms of Fragaria chiloensis ssp. chiloensis"}]},{"@id":"https://cir.nii.ac.jp/crid/1572261549947959680","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"A simple protocol for transient gene expression in ripe fleshy fruit mediated by Agrobacterium"}]},{"@id":"https://cir.nii.ac.jp/crid/1572543026610363776","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Gene expression analysis of strawberry achene and receptacle maturation using DNA microarrays"}]},{"@id":"https://cir.nii.ac.jp/crid/1573105975622179200","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"The strawberry fruit Fra a allergen functions in flavonoid biosynthesis"}]},{"@id":"https://cir.nii.ac.jp/crid/1573105975622181376","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Characterization of major enzymes and genes involved in flavonoid and proanthocyanidin biosynthesis during fruit development in strawberry (Fragaria×ananassa)"}]},{"@id":"https://cir.nii.ac.jp/crid/1573668925575602304","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Developmental, genetic and environmental factors affect the expression of flavonoid genes, enzymes and metabolites in strawberry fruits"}]}],"dataSourceIdentifier":[{"@type":"JALC","@value":"oai:japanlinkcenter.org:1000764208"},{"@type":"JALC","@value":"oai:japanlinkcenter.org:9100001161"},{"@type":"NDL_SEARCH","@value":"oai:ndlsearch.ndl.go.jp:R000000004-I023784456"},{"@type":"CROSSREF","@value":"10.5511/plantbiotechnology.12.0406a"},{"@type":"CROSSREF","@value":"10.5511/plantbiotechnology.12.406a"},{"@type":"CIA","@value":"10030804744"},{"@type":"CIA","@value":"130001883169"},{"@type":"OPENAIRE","@value":"doi_dedup___::f6a653612e51ced1aa15e75ee0ab8706"},{"@type":"CROSSREF","@value":"10.1007/s00425-014-2228-6_references_DOI_1AORDCQ58WcX5DOydrMFmWAZfBS"},{"@type":"CROSSREF","@value":"10.1007/s10265-013-0582-2_references_DOI_1AORDCQ58WcX5DOydrMFmWAZfBS"}]}