{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1390282679304255232.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.5511/plantbiotechnology.13.0128a"}},{"identifier":{"@type":"COI","@value":"1:CAS:528:DC%2BC3sXhtlCqsL3I"}},{"identifier":{"@type":"NDL_BIB_ID","@value":"024676569"}},{"identifier":{"@type":"URI","@value":"http://id.ndl.go.jp/bib/024676569"}},{"identifier":{"@type":"URI","@value":"https://ndlsearch.ndl.go.jp/books/R000000004-I024676569"}},{"identifier":{"@type":"URI","@value":"https://dl.ndl.go.jp/pid/10205139"}},{"identifier":{"@type":"NAID","@value":"10031184117"}}],"dc:title":[{"@language":"en","@value":"Establishment of <i>Agrobacterium</i>-mediated genetic transformation system in <i>Dahlia</i>"},{"@value":"Establishment of Agrobacterium-mediated genetic transformation system in Dahlia"}],"dc:language":"en","description":[{"type":"abstract","notation":[{"@language":"en","@value":"An efficient system for <i>Agrobacterium</i>-mediated transformation was established in dahlia (<i>Dahlia</i>×<i>pinnata</i>) ‘Yamatohime’. Mass of shoot primordia (MSP) induced on MS medium supplemented with 10 mg l<sup>−1</sup> TDZ were inoculated with <i>Agrobacterium tumefaciens</i> strain EHA101 (pIG121-Hm) harboring both β-glucuronidase (GUS) and hygromycin resistant genes. After 2 days of co-cultivation, the MSP were transferred to a selection medium containing hygromycin with meropenem for bacterial elimination. Shoots were successfully regenerated from survived MSP on hormone-free medium without hygromycin and they rooted on hormone-free medium containing hygromycin. The hygromycin-resistant plants thus obtained showed histochemical blue staining for GUS. Transformation of plants was confirmed by PCR and Southern blot analyses. Transgenic ‘Kokucho’ were also produced by using the same transformation procedure, suggesting wide applicability of this <i>Agrobacterium</i>-mediated transformation procedure for other dahlia cultivars."}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1410282679304255233","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000261673310"}],"foaf:name":[{"@language":"en","@value":"Otani Yuko"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Graduate School of Horticulture, Chiba University"}]},{"@id":"https://cir.nii.ac.jp/crid/1410001204326819203","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000000953202"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/chindongpoh"}],"foaf:name":[{"@language":"en","@value":"Chin Dong Poh"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Graduate School of Horticulture, Chiba University"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282679304255234","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000261673312"}],"foaf:name":[{"@language":"en","@value":"Mii Masahiro"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Graduate School of Horticulture, Chiba University"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"13424580"},{"@type":"LISSN","@value":"13424580"},{"@type":"EISSN","@value":"13476114"},{"@type":"CODEN","@value":"PLBIF6"},{"@type":"NDL_BIB_ID","@value":"000000157745"},{"@type":"ISSN","@value":"13424580"},{"@type":"NCID","@value":"AA11250821"}],"prism:publicationName":[{"@language":"en","@value":"Plant Biotechnology"},{"@language":"ja","@value":"Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ"},{"@language":"en","@value":"Plant Biotechnology"},{"@language":"ja","@value":"Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ"}],"dc:publisher":[{"@language":"en","@value":"Japanese Society for Plant Biotechnology"},{"@language":"ja","@value":"日本植物バイオテクノロジー学会"}],"prism:publicationDate":"2013","prism:volume":"30","prism:number":"2","prism:startingPage":"135","prism:endingPage":"139"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","url":[{"@id":"http://id.ndl.go.jp/bib/024676569"},{"@id":"https://ndlsearch.ndl.go.jp/books/R000000004-I024676569"},{"@id":"https://dl.ndl.go.jp/pid/10205139"}],"availableAt":"2013","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=%3Ci%3EAgrobacterium%20tumefaciens%3C/i%3E","dc:title":"<i>Agrobacterium tumefaciens</i>"},{"@id":"https://cir.nii.ac.jp/all?q=%3Ci%3EDahlia%3C/i%3E%C3%97%3Ci%3Epinnata%3C/i%3E","dc:title":"<i>Dahlia</i>×<i>pinnata</i>"},{"@id":"https://cir.nii.ac.jp/all?q=ornamental%20plant","dc:title":"ornamental plant"},{"@id":"https://cir.nii.ac.jp/all?q=shoot%20regeneration","dc:title":"shoot regeneration"},{"@id":"https://cir.nii.ac.jp/all?q=transformation.","dc:title":"transformation."}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360011143739579520","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Regeneration of herbicide-tolerant black locust transgenic plants by SAAT"}]},{"@id":"https://cir.nii.ac.jp/crid/1360011146543214464","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Additional virulence genes and sonication enhance Agrobacterium tumefaciens-mediated loblolly pine transformation"}]},{"@id":"https://cir.nii.ac.jp/crid/1361699993837647360","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Efficient selection and regeneration of creeping bentgrass transformants following particle bombardment"}]},{"@id":"https://cir.nii.ac.jp/crid/1361981470409835136","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The use of the polymerase 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