{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1390282679332072960.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.2116/bunsekikagaku.28.8_490"}},{"identifier":{"@type":"NDL_BIB_ID","@value":"2061699"}},{"identifier":{"@type":"URI","@value":"http://id.ndl.go.jp/bib/2061699"}},{"identifier":{"@type":"URI","@value":"https://ndlsearch.ndl.go.jp/books/R000000004-I2061699"}},{"identifier":{"@type":"URI","@value":"https://dl.ndl.go.jp/pid/10888936"}},{"identifier":{"@type":"NAID","@value":"110002908048"}},{"identifier":{"@type":"URI","@value":"http://id.ndl.go.jp/digimeta/10888936"}},{"identifier":{"@type":"URI","@value":"http://www.jstage.jst.go.jp/article/bunsekikagaku1952/28/8/28_8_490/_pdf"}},{"identifier":{"@type":"URI","@value":"http://dl.ndl.go.jp/info:ndljp/pid/10888936"}},{"identifier":{"@type":"URI","@value":"https://search.jamas.or.jp/link/ui/1980059830"}}],"dc:title":[{"@value":"グアヤコールエストロジェン類の薄層クロマトグラフィーによる分離定量"},{"@language":"en","@value":"Separation and estimation of guaiacol estrogens by thin-layer chromatography"},{"@language":"ja-Kana","@value":"グアヤコールエストロジェンルイ ノ ハクソウ クロマトグラフィー ニヨル ブン"}],"dcterms:alternative":[{"@value":"ステロイドの臨床分析(第10報)"}],"dc:language":"ja","description":[{"type":"abstract","notation":[{"@language":"en","@value":"Two couples of biological guaiacol estrogen isomers and a couple of synthetic ones were separated satisfactory by thin-layer chromatography by using continuous developing technique. The chromatographic plates were Merck silica gel 60 F<SUB>254</SUB> (No. 5715) and spotted plates were developed continuously. Following solvents were used: benzene for the separation of 2-methoxy-estrone (II) and 2-hydroxyestrone-3-methyl ether (III), 2% acetone in benzene for the mixture of 2-methoxyestradiol(V) and 2-hydroxyestradiol-3-methyl ether (VI), and 5% acetone in benzene for 2-methoxy-3, 17 β-dihydroxyestra-1, 3, 5(10)-trien-6-one (VIII) and 3-methoxy-2, 17β-dihydroxyestra-1, 3, 5 (10)-trien-6-one (IX). Combination of the present separation technique with thin-layer chromato-scanner made possible for the estimation of guaiacol estrogens. Calibration curves between the spotted steroid amounts and peak area on chromatograms were shown to be linear in the range of (0.510.0) μg for II, III, V, and VI, and (0.210) μg for VIII and IX. Estimation of radioactive guaiacols obtained by the incubation of catechol estrogens with rat liver catechol <I>O</I>-methyl transferase in the presence of <I>S</I>-adenosyl-L-methionine (<SUP>3</SUP>H<SUB>3</SUB>C) was also possible by using radio thin-layer chromato-scanner."},{"@value":"メチル化酵素によって,カテコールエストロジェン類から生ずるグアヤコールエストロジェン異性体類を,シリカゲルプレートを固定相に用いて連続展開法による薄層クロマトグラフィー(TLC)分離を行い,次いで薄層クロマトスキャンナーを併用することにより,それらステロイドの定量法を確立した.用いたステロイドは,2-メトキシエストロン(II)と2-ヒドロキシエストロン-3-メチルエーテル(III),2-メトキシエストラジオール(V)と2-ヒドロキシエストラジオール-3-メチルエーテル(VI),及び2-メトキシ-3,17β-ジヒドロキシエストラ-1,3,5(10)-トリエン-6-オン(VIII)と3-メトキシ-2,17β-ジヒドロキシエストラ-1,3,5(10)-トリエン-6-オン(IX)の3組の異性体で,それらのTLC分離のために次のような展開溶媒を用いた:ベンゼン,2%アセトン/ベンゼン,及び5%アセトン/ベンゼン.<BR>次にこれらステロイドのTLCによる定量を検討した.プレートにスポットした試料の量とスキャンナーより得られるクロマトグラム上のピーク面積の強度(重量法)との間には,II,III,V,及びVIの場合には(0.5～10)μg,VIII及びIXの場合には(0.2～10)μgの範囲で直線性を示し,この間での定量が可能である.<BR>本分析法はラジオ薄層クロマトスキャンナーと併用すれば,放射性のグアヤコールエストロジェン類の定性並びに定量も可能である."}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1030003658334849794","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"80088864"},{"@type":"NRID","@value":"1000080088864"},{"@type":"NRID","@value":"9000392640412"},{"@type":"NRID","@value":"9000254220365"},{"@type":"NRID","@value":"9000254675780"},{"@type":"NRID","@value":"9000392638357"},{"@type":"NRID","@value":"9000254674073"},{"@type":"NRID","@value":"9000254209783"},{"@type":"NRID","@value":"9000254709750"},{"@type":"NRID","@value":"9000254689449"},{"@type":"NRID","@value":"9000392638354"},{"@type":"NRID","@value":"9000392671927"},{"@type":"NRID","@value":"9000254224173"},{"@type":"NRID","@value":"9000392642691"},{"@type":"NRID","@value":"9000392640212"},{"@type":"NRID","@value":"9000254681556"},{"@type":"NRID","@value":"9000254675847"},{"@type":"NRID","@value":"9000254671162"},{"@type":"NRID","@value":"9000254694551"},{"@type":"NRID","@value":"9000254219988"},{"@type":"NRID","@value":"9000254210872"},{"@type":"NRID","@value":"9000254240833"},{"@type":"NRID","@value":"9000254692533"},{"@type":"NRID","@value":"9000254688863"},{"@type":"NRID","@value":"9000254209759"},{"@type":"NRID","@value":"9000254210317"},{"@type":"NRID","@value":"9000253210646"},{"@type":"NRID","@value":"9000254692285"},{"@type":"NRID","@value":"9000392651341"},{"@type":"NRID","@value":"9000021837004"},{"@type":"NRID","@value":"9000392667334"},{"@type":"NRID","@value":"9000254210224"},{"@type":"NRID","@value":"9000254671303"},{"@type":"NRID","@value":"9000254210578"},{"@type":"NRID","@value":"9000254682927"},{"@type":"NRID","@value":"9000392665761"},{"@type":"NRID","@value":"9000020795852"},{"@type":"NRID","@value":"9000392672182"},{"@type":"NRID","@value":"9000254682914"},{"@type":"NRID","@value":"9000392652652"},{"@type":"NRID","@value":"9000392666834"},{"@type":"NRID","@value":"9000254682150"},{"@type":"NRID","@value":"9000392652675"},{"@type":"NRID","@value":"9000254671730"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0021900"}],"foaf:name":[{"@language":"en","@value":"YOSHIZAWA Itsuo"},{"@language":"ja","@value":"吉沢 逸雄"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Hokkaido Institute of Pharmaceutical Sciences"},{"@language":"ja","@value":"北海道薬科大学薬学科薬品分析学教室"}]},{"@id":"https://cir.nii.ac.jp/crid/1030003658343003648","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"60050548"},{"@type":"NRID","@value":"1000060050548"},{"@type":"NRID","@value":"9000004410840"},{"@type":"NRID","@value":"9000254687001"},{"@type":"NRID","@value":"9000254672529"},{"@type":"NRID","@value":"9000254679668"},{"@type":"NRID","@value":"9000254674193"},{"@type":"NRID","@value":"9000020323174"},{"@type":"NRID","@value":"9000254680142"},{"@type":"NRID","@value":"9000254213696"},{"@type":"NRID","@value":"9000254211900"},{"@type":"NRID","@value":"9000020795855"},{"@type":"NRID","@value":"9000254676952"},{"@type":"NRID","@value":"9000256040911"}],"foaf:name":[{"@language":"en","@value":"NAKAGAWA Akiko"},{"@language":"ja","@value":"中川 明子"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Hokkaido Institute of Pharmaceutical Sciences"},{"@language":"ja","@value":"北海道薬科大学薬学科薬品分析学教室"}]},{"@id":"https://cir.nii.ac.jp/crid/1410009221450337408","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000020795860"}],"foaf:name":[{"@language":"en","@value":"OH'UCHI Ryoko"},{"@language":"ja","@value":"大内 涼子"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Hokkaido Institute of Pharmaceutical Sciences"},{"@language":"ja","@value":"北海道薬科大学薬学科薬品分析学教室"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"05251931"},{"@type":"NDL_BIB_ID","@value":"000000021279"},{"@type":"ISSN","@value":"05251931"},{"@type":"LISSN","@value":"05251931"},{"@type":"NCID","@value":"AN00222633"}],"prism:publicationName":[{"@language":"ja","@value":"分析化学"},{"@language":"en","@value":"BUNSEKI KAGAKU"},{"@language":"en","@value":"BUNSEKI KAGAKU"},{"@language":"en","@value":"Bunseki kagaku"},{"@language":"ja","@value":"分析化学"}],"dc:publisher":[{"@language":"en","@value":"The Japan Society for Analytical Chemistry"},{"@language":"ja","@value":"公益社団法人 日本分析化学会"}],"prism:publicationDate":"1979","prism:volume":"28","prism:number":"8","prism:startingPage":"490","prism:endingPage":"494"},"reviewed":"false","url":[{"@id":"http://id.ndl.go.jp/bib/2061699"},{"@id":"https://ndlsearch.ndl.go.jp/books/R000000004-I2061699"},{"@id":"https://dl.ndl.go.jp/pid/10888936"},{"@id":"http://id.ndl.go.jp/digimeta/10888936"},{"@id":"http://www.jstage.jst.go.jp/article/bunsekikagaku1952/28/8/28_8_490/_pdf"},{"@id":"http://dl.ndl.go.jp/info:ndljp/pid/10888936"},{"@id":"https://search.jamas.or.jp/link/ui/1980059830"}],"availableAt":"1979","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Catechol%20estrogen","dc:title":"Catechol estrogen"},{"@id":"https://cir.nii.ac.jp/all?q=Continuous%20developing%20method","dc:title":"Continuous developing method"},{"@id":"https://cir.nii.ac.jp/all?q=Guaiacol%20estrogen","dc:title":"Guaiacol estrogen"},{"@id":"https://cir.nii.ac.jp/all?q=Radio%20thin-layer%20chromatoscanner","dc:title":"Radio thin-layer chromatoscanner"},{"@id":"https://cir.nii.ac.jp/all?q=Thin-layer%20chromatography","dc:title":"Thin-layer chromatography"},{"@id":"https://cir.nii.ac.jp/all?q=Thin-layer%20chromatoscanner","dc:title":"Thin-layer chromatoscanner"}],"dataSourceIdentifier":[{"@type":"JALC","@value":"oai:japanlinkcenter.org:0034455456"},{"@type":"NDL_SEARCH","@value":"oai:ndlsearch.ndl.go.jp:R000000004-I2061699"},{"@type":"NDL_SEARCH","@value":"oai:ndlsearch.ndl.go.jp:R100000039-I10888936"},{"@type":"CROSSREF","@value":"10.2116/bunsekikagaku.28.8_490"},{"@type":"NDL_DC","@value":"info:ndljp/pid/10888936"},{"@type":"CIA","@value":"110002908048"}]}