On the precision of the automatic amino acid analyzer

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Bibliographic Information

Other Title
  • 自動アミノ酸分析計の精度について
  • ジドウ アミノサン ブンセキケイ ノ セイド ニ ツイテ
Published
1966
DOI
  • 10.2116/bunsekikagaku.15.3
Publisher
The Japan Society for Analytical Chemistry

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Description

The reproducibility of the automatic analysis of amino acids is examined by the repeated determinations. i) The ordinate axis of the recorder sheet of the automatic amino acid analyzer such as HITACHI Type KLA-2 should be longer than 25 cm. ii) Small variations in the elution time of each amino acid do not affect the result of determination. iii) The shape of the peak gives a greater effect on the result, and a well-defined Gaussian curve is required to obtain high reproducibility. iv) The absorbance ratios of 440 mμ/570 mμ, and 440 mμ/650 mμ for the reaction products of 52 amino acid components with ninhydrin were determined. The reproducibilities of the estimated ratios are given for 20 standard amino acids.

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 15 (1), 3-9, 1966

    The Japan Society for Analytical Chemistry

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