Development of Functional Surfaces by Layered Thin Films Containing Proteins

SATO Katsuhiko, ANZAI Jun-ichi

doi:10.2116/bunsekikagaku.56.387

Protein thin films containing avidin and lectin have been prepared on solid surfaces to develop devices sensitive to pH and biomolecules. Concanavalin A (Con A), one of the glucose- and mannose-binding lectins, and glycogen were alternately deposited onto the surface of a quartz slide to prepare layer-by-layer (LbL) thin films, through strong Con A-glycogen binding. Con A-glycogen LbL films are sensitive to sugars, and can be disintegrated in response to sugars, depending on the type of sugars and the concentration. Avidin and biotin (or iminobiotin)-modified polymers were used for constructing LbL thin films and the response to the pH and biotin analogues was evaluated. LbL films composed of avidin and iminobiotin-modified polymer can be disintegrated upon changing the pH of the media in which the films are immersed. LbL films were decomposed when the solution was acidified, while in basic solutions the films were stable. In a similar way, LbL films deposited onto the surface of electrodes that were sensitive to an electrode potential. LbL films were disintegrated upon applying positive potentials (1 V or lower) to the electrode due to acidification of the local pH at the electrode-film interface, arising from the electrolysis of water. LbL films composed of avidin and iminobiotin-modified polymer were also decomposed by adding biotin and analogues in solution. Thus, a possible use of LbL films containing Con A and avidin in biosensing and delivery was suggested.<br>

Development of Functional Surfaces by Layered Thin Films Containing Proteins

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