Induction of chorismate mutase by an elicitor in oat leaves

DOI
  • Matsukawa Tetsuya
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University:CREST, Japan Science and Technology Corporation (JST)
  • Ishihara Atsushi
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University:CREST, Japan Science and Technology Corporation (JST)
  • Iwamura Hajime
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University:CREST, Japan Science and Technology Corporation (JST)

Bibliographic Information

Other Title
  • 2 エンバクにおけるエリシターによるコリスミ酸ムターゼ誘導

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Description

Avenanthramides, a series of substituted N-cinnamoylanthranilate derivatives, are phytoalexins in oats. Hydroxycinnamate and anthranilate are derived from the shikimate pathway which branches at chorismate. Chorismate mutase (CM) is the first enzyme of the branch that leads to phenylalanine and phenylpropanoid biosynthesis. The induction of CM was investigated in primary oat leaves using oligo-N-acetylchitooligosaccharides as elicitors. The CM activity started to increase 6 hr after elictitation and reached a maximum by 9 hr. Among the oligo-N-acetylchitooligosaccharides tested, tetra-, penta-, and hexasaccharides effectively induced the enzyme activity in a dose-dependent manner. The activity was separated into two major peaks by anion exchange chromatography indicating that CM consists of at least two isoforms. Comparison of untreated and elicitor-treated leaves revealed that only one CM isoform was responsive to the elicitor. These results suggest a regulatory role of CM in avenanthramide biosynthesis.

Journal

Details 詳細情報について

  • CRID
    1390282679399532800
  • NII Article ID
    110001853506
  • DOI
    10.18978/jscrpanb.35.0_11
  • ISSN
    21896313
    09191887
  • Text Lang
    ja
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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