{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1390282679406470656.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1046/j.1444-2906.2003.00599.x"}},{"identifier":{"@type":"NAID","@value":"130003904011"}}],"dc:title":[{"@language":"en","@value":"Follicle-stimulating hormone .BETA., luteinizing hormone .BETA. and glycoprotein hormone .ALPHA. subunit mRNA levels in artificially maturing Japanese eel Anguilla japonica and naturally maturing New Zealand longfinned eel Anguilla dieffenbachii."},{"@value":"Follicle-stimulating hormone, luteinizing hormone and glycoprotein hormone subunit mRNA levels in artificially maturing Japanese eel Anguilla japonica and naturally maturing New Zealand longfinned eel Anguilla dieffenbachia"}],"dc:language":"en","description":[{"type":"abstract","notation":[{"@language":"en","@value":"cDNAs for three subunits of gonadotropin, namely follicle-stimulating hormone (FSH) β, luteinizing hormone (LH) β and glycoprotein hormone (GP) α, of the New Zealand longfinned eel <i>Anguilla dieffenbachii</i> were cloned. Based on their nucleotide sequences, which were more than 98% identical to those of the Japanese eel <i>Anguilla japonica</i>, a real-time reverse transcription-polymerase chain reaction method for the quantitative assay of gonadotropin subunit mRNAs of the two eel species was developed using the same primer sets. Accordingly, changes in pituitary gonadotropin mRNA levels were analyzed in female Japanese eels induced to develop artificially by salmon pituitary homogenate (SPH) treatment and in naturally maturing female New Zealand longfinned eels collected from the wild. In Japanese eels, the FSH β mRNA level was very high at the previtellogenic stage (PV) and decreased remarkably after SPH injection, whereas the reverse was seen in New Zealand longfinned eels. The mRNA levels of LH β and GP α increased during gonadal development in both species, albeit to a much greater extent in Japanese eels. As the Japanese and New Zealand longfinned eels are closely related species of the same genus, the different gonadotropin expression patterns are likely to reflect differences in maturational conditions rather than species. Our findings are indicative of aberrant gonadal development and may explain the poor quality eggs obtained from artificially matured Japanese eels."}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1410282679406470660","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000254577566"}],"foaf:name":[{"@language":"en","@value":"LOKMAN P MARK"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Zoology, University of Otago, Dunedin, New Zealand"},{"@language":"en","@value":"Department of Marine Science, University of 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KOJI"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Division of Marine Biosciences, Graduate School of Fisheries, Hokkaido University"},{"@language":"en","@value":"Division of Molecular Interaction, Institute for Genetic Medicine, Hokkaido University Graduate School of Medicine"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282679406470665","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000254577567"}],"foaf:name":[{"@language":"en","@value":"YOUNG GRAHAM"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Zoology, University of Otago, Dunedin, New Zealand"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282679406470661","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000254577568"}],"foaf:name":[{"@language":"en","@value":"OZAKI YUICHI"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Division of Marine Biosciences, Graduate School of Fisheries, Hokkaido 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YASUTOSHI"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo"},{"@language":"en","@value":"National Research Institute of Aquaculture, Fisheries Research Agency"}]},{"@id":"https://cir.nii.ac.jp/crid/1420282801196894592","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"40231930"},{"@type":"NRID","@value":"1000040231930"},{"@type":"NRID","@value":"9000364702481"},{"@type":"NRID","@value":"9000254577751"},{"@type":"NRID","@value":"9000254572680"},{"@type":"NRID","@value":"9000254144853"},{"@type":"NRID","@value":"9000342792081"},{"@type":"NRID","@value":"9000402233561"},{"@type":"NRID","@value":"9000410265747"},{"@type":"NRID","@value":"9000303997810"},{"@type":"NRID","@value":"9000254143527"},{"@type":"NRID","@value":"9000018337397"},{"@type":"NRID","@value":"9000351082864"},{"@type":"NRID","@value":"9000254575405"},{"@type":"NRID","@value":"9000254574384"},{"@type":"NRID","@value":"9000254574226"},{"@type":"NRID","@value":"9000254141962"},{"@type":"NRID","@value":"9000254575839"},{"@type":"NRID","@value":"9000254144130"},{"@type":"NRID","@value":"9000415230544"},{"@type":"NRID","@value":"9000254575645"},{"@type":"NRID","@value":"9000242444278"},{"@type":"NRID","@value":"9000254145398"},{"@type":"NRID","@value":"9000413934299"},{"@type":"NRID","@value":"9000254572849"},{"@type":"NRID","@value":"9000292872248"},{"@type":"NRID","@value":"9000356913644"},{"@type":"NRID","@value":"9000254574635"},{"@type":"NRID","@value":"9000254145230"},{"@type":"NRID","@value":"9000254143893"},{"@type":"NRID","@value":"9000254142908"},{"@type":"NRID","@value":"9000254144166"},{"@type":"NRID","@value":"9000254577210"},{"@type":"NRID","@value":"9000254572874"},{"@type":"NRID","@value":"9000254577564"},{"@type":"NRID","@value":"9000406211830"},{"@type":"NRID","@value":"9000410998425"},{"@type":"NRID","@value":"9000398624694"},{"@type":"NRID","@value":"9000361706799"},{"@type":"NRID","@value":"9000347127722"},{"@type":"NRID","@value":"9000394470580"},{"@type":"NRID","@value":"9000010528863"},{"@type":"NRID","@value":"9000415201579"},{"@type":"NRID","@value":"9000412248578"},{"@type":"NRID","@value":"9000254575447"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0166779"}],"foaf:name":[{"@language":"en","@value":"ADACHI SHINJI"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Division of Marine Biosciences, Graduate School of Fisheries, Hokkaido University"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282679406470658","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000254577565"}],"foaf:name":[{"@language":"en","@value":"YAMAUCHI KOHEI"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Division of Marine Biosciences, Graduate School of Fisheries, Hokkaido University"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282679406470659","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000254577570"}],"foaf:name":[{"@language":"en","@value":"OKUMURA HIROMI"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Division of Marine Biosciences, Graduate School of Fisheries, Hokkaido University"},{"@language":"en","@value":"Protein Research Group, RIKEN Genomic Sciences Center (GSC)"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282679406470657","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000254577571"}],"foaf:name":[{"@language":"en","@value":"KAZETO YUKINORI"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Division of Marine Biosciences, Graduate School of Fisheries, Hokkaido University"},{"@language":"en","@value":"Center of Marine Biotechnology, University of Maryland, Biotechnology Institute"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282679406470656","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000254577573"}],"foaf:name":[{"@language":"en","@value":"AIDA KATSUMI"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"09199268"},{"@type":"EISSN","@value":"14442906"}],"prism:publicationName":[{"@language":"en","@value":"Fisheries science"},{"@language":"ja","@value":"Ｆｉｓｈｅｒｉｅｓ　ｓｃｉｅｎｃｅ"},{"@language":"en","@value":"Fisheries science"},{"@language":"ja","@value":"Ｆｉｓｈｅｒｉｅｓ　ｓｃｉｅｎｃｅ"}],"dc:publisher":[{"@language":"en","@value":"The Japanese Society of Fisheries Science"},{"@language":"ja","@value":"公益社団法人 日本水産学会"}],"prism:publicationDate":"2003","prism:volume":"69","prism:number":"1","prism:startingPage":"146","prism:endingPage":"153"},"reviewed":"false","availableAt":"2003","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=gene%20expression","dc:title":"gene expression"},{"@id":"https://cir.nii.ac.jp/all?q=gonadal%20maturation","dc:title":"gonadal maturation"},{"@id":"https://cir.nii.ac.jp/all?q=gonadotropin","dc:title":"gonadotropin"},{"@id":"https://cir.nii.ac.jp/all?q=Japanese%20eel","dc:title":"Japanese eel"},{"@id":"https://cir.nii.ac.jp/all?q=New%20Zealand%20longfinned%20eel","dc:title":"New Zealand longfinned eel"},{"@id":"https://cir.nii.ac.jp/all?q=real-time%20PCR","dc:title":"real-time PCR"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050869456409017088","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Histological characteristics of the oocyte chorion in wild post-spawning and artificially matured Japanese eels Anguilla japonica"}]},{"@id":"https://cir.nii.ac.jp/crid/1360005520779671936","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Reproductive physiological characteristics of tropical Celebes eels <scp><i>Anguilla celebesensis</i></scp> in relation to downstream migration and ovarian development"}]},{"@id":"https://cir.nii.ac.jp/crid/1360011143762766208","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Changes in serum steroid hormones and steroidogenic ability of ovarian follicles during artificial maturation of cultivated Japanese eel, Anguilla japonica"}]},{"@id":"https://cir.nii.ac.jp/crid/1360011145077561088","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Growth Hormone (GH) and Gonadotropin Subunit Gene Expression and Pituitary and Plasma Changes during Spermatogenesis and Oogenesis in Rainbow Trout (Oncorhynchus mykiss)"}]},{"@id":"https://cir.nii.ac.jp/crid/1360011146129337728","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Morphometric changes, gonad histology, and fecundity estimates in migrating New Zealand freshwater eels (\n                    <i>Anguilla</i>\n                    spp.)"}]},{"@id":"https://cir.nii.ac.jp/crid/1360292621467945728","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Regulation of the type-II gonadotrophin α and β subunit mRNAs by œstradiol and testosterone in the European eel"}]},{"@id":"https://cir.nii.ac.jp/crid/1360574094305315584","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Duality of Gonadotropin in a Primitive Teleost, Japanese Eel (Anguilla japonica)"}]},{"@id":"https://cir.nii.ac.jp/crid/1360855568509113984","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Effects of sex steroids on gonadotropin (FSH and LH) regulation in coho salmon (Oncorhynchus kisutch)"}]},{"@id":"https://cir.nii.ac.jp/crid/1361418518394315136","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Molecular cloning of the cDNAs encoding pituitary glycoprotein hormone α- and gonadotropin IIβ-subunits of the Japanese eel, Anguilla japonica, and increase in their mRNAs during ovarian development induced by injection of chum salmon pituitary homogenate"}]},{"@id":"https://cir.nii.ac.jp/crid/1361418518808503552","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Molecular Cloning and Characterization of Japanese Eel Ovarian P450c17 (CYP17) cDNA"}]},{"@id":"https://cir.nii.ac.jp/crid/1361694594159516032","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Changes in Sex Steroids and Ovarian Steroidogenic Enzyme mRNA Levels in Artificially Maturing Japanese Eel (Anguilla japonica) and Naturally Maturing New Zealand Longfin Eel (Anguilla dieffenbachii) during Vitellogenesis"}]},{"@id":"https://cir.nii.ac.jp/crid/1361699994313860480","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Gonad histology and plasma steroid profiles in wild New Zealand freshwater eels (Anguilla dieffenbachii and A. australis) before and at the onset of the natural spawning migration. 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Females*"}]},{"@id":"https://cir.nii.ac.jp/crid/1361981470209904256","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Androgens stimulate gonadotropin-II β-subunit in eel pituitary cells in vitro"}]},{"@id":"https://cir.nii.ac.jp/crid/1362825894955814912","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"In vitro biosynthesis of oestradiol-17β and 17α,20gb-dihydroxy-4-pregnen-3-one by vitellogenic ovarian follicles from migrating New Zealand longfinned eels (Anguilla dieffenbachii)"}]},{"@id":"https://cir.nii.ac.jp/crid/1363107368999243648","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Seasonal Changes in mRNA Levels of Gonadotropin and Thyrotropin Subunits in the Goldfish,Carassius auratus"}]},{"@id":"https://cir.nii.ac.jp/crid/1363107370986778240","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Changes in transcription of pituitary glycoprotein hormone α and gonadotropin IIβ subunits during ovarian development induced by repeated injections of salmon pituitary homogenate in the Japanese eel, Anguilla japonica"}]},{"@id":"https://cir.nii.ac.jp/crid/1363388843253522048","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"A novel method for real time quantitative RT-PCR."}]},{"@id":"https://cir.nii.ac.jp/crid/1363388845057878016","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Cultivation of larvae of Japanese eel"}]},{"@id":"https://cir.nii.ac.jp/crid/1363388845213149952","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Steroidogenic activities of two distinct salmon gonadotropins"}]},{"@id":"https://cir.nii.ac.jp/crid/1363670318662780800","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Isolation and Characterization of Two Coho Salmon Gonadotropins, GTH I and GTH II1"}]},{"@id":"https://cir.nii.ac.jp/crid/1363670319173642752","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Unique Expression of Gonadotropin-I and -II Subunit Genes in Male and Female Red Seabream (Pagrus major) During Sexual Maturation1"}]},{"@id":"https://cir.nii.ac.jp/crid/1363670320302473984","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Reproductive development of male and female tilapia hybrids (Oreochromis niloticus × O. aureus) and changes in mRNA levels of gonadotropin (GtH) Iβ and IIβ subunits"}]},{"@id":"https://cir.nii.ac.jp/crid/1364233269742649088","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Evolution of the Freshwater Eels"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204428348288","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Effects of sex steroids on the mRNA levels of gonadotropin subunits in juvenile and ovariectomized goldfish Carassius auratus."}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679404813568","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Differential expression of two gonadotropin (GTH) .BETA. subunit genes during ovarian maturation induced by repeated injection of salmon GTH in the Japanese eel Anguilla japonica."},{"@value":"Differential expressin of two gonadotropin(GTH) β subunit genes during ovarian maturation induced by repeated injection of salmon GTH in the Japanese eel Anguilla japonica"},{"@language":"ja-Kana","@value":"Differential expressin of two gonadotropin GTH ベータ subunit genes during ovarian maturation induced by repeated injection of salmon GTH in the Japanese eel Anguilla japonica"}]}],"dataSourceIdentifier":[{"@type":"JALC","@value":"oai:japanlinkcenter.org:0016304478"},{"@type":"CROSSREF","@value":"10.1046/j.1444-2906.2003.00599.x"},{"@type":"CIA","@value":"130003904011"},{"@type":"OPENAIRE","@value":"doi_dedup___::b1e7e194b0a664c0d5fbe54a4def91fb"},{"@type":"CROSSREF","@value":"10.1007/s12562-015-0851-1_references_DOI_Gc29wsiFLk9CgfHRNlKuZqYzy5N"},{"@type":"CROSSREF","@value":"10.1111/jfb.14231_references_DOI_Gc29wsiFLk9CgfHRNlKuZqYzy5N"},{"@type":"CROSSREF","@value":"10.3390/fishes4040052_references_DOI_Gc29wsiFLk9CgfHRNlKuZqYzy5N"}]}