Detection Method for Aquatic Bacteria of the Fingers, as a Potential Origin of the Aqueous Solution Contamination
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- OSONO EIICHI
- Koshigaya Ohbukuro Clinic Microbiology and Immunology, Nippon Medical School
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- HONDA KAZUMI
- Koshigaya Ohbukuro Clinic
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- INOUE YUKI
- Koshigaya Ohbukuro Clinic
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- NOROSE YOSHIHIKO
- Microbiology and Immunology, Nippon Medical School
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- TAKAHASHI MEGUMI
- Microbiology and Immunology, Nippon Medical School
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- ICHIMURA KYOKO
- Koshigaya Ohbukuro Clinic
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- KAMANO CHISAKO
- Koshigaya Ohbukuro Clinic
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- SHINYA EIJI
- Microbiology and Immunology, Nippon Medical School
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- TAKAKU SHUN
- Microbiology and Immunology, Nippon Medical School
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- OKAMATSU KENTARO
- Komagome Aoba Clinic
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- KAWAMOTO SHINYA
- Nephrology, Dokkyo Medical University, Koshigaya Hospital
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- TAKIZAWA HIDEAKI
- Social Medicene, Tokyo University of Pharmacy and Life Science
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- TAKAHASHI HIDEMI
- Microbiology and Immunology, Nippon Medical School
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Abstract
<p> Aquatic bacteria were isolated from the hands of working staffs by an adapted culture protocol. When the sample solution obtained by the“ glove juice method” was incubated for 3 days at room temperature, viable cell counts increased up to 105-fold, and the majority of the isolated colonies were shown to be Gram-negative aquatic bacteria, which carry the risk of contaminating water. Using R2A medium, coagulase-negative staphylococci were the dominant microbes immediately after recovery from the hands. Here it was revealed that bacteria of the phylum Proteobacteria isolated from the hand can be the causative bacteria of aqueous contamination. This modification in the GJ method may be useful as an effective training protocol to demonstrate the importance of hand hygiene and clean operation for aseptic manufacturing. </p>
Journal
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- Biocontrol Science
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Biocontrol Science 22 (1), 61-65, 2017
The Society for Antibacterial and Antifungal Agents, Japan