{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1390282679474287232.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1248/jhs.50.511"}},{"identifier":{"@type":"NDL_BIB_ID","@value":"7097538"}},{"identifier":{"@type":"URI","@value":"http://id.ndl.go.jp/bib/7097538"}},{"identifier":{"@type":"URI","@value":"https://ndlsearch.ndl.go.jp/books/R000000004-I7097538"}},{"identifier":{"@type":"URI","@value":"https://dl.ndl.go.jp/pid/8717535"}},{"identifier":{"@type":"URI","@value":"http://www.jstage.jst.go.jp/article/jhs/50/5/50_5_511/_pdf"}},{"identifier":{"@type":"NAID","@value":"110003609196"}},{"identifier":{"@type":"URI","@value":"https://search.jamas.or.jp/link/ui/2006059976"}}],"dc:title":[{"@language":"en","@value":"Development of Standardized in Vitro Assay System for Estrogen Receptors and Species Specificity of Binding Ability of 4-Nonylphenol and p-Octylphenol"}],"dc:language":"en","description":[{"type":"abstract","notation":[{"@language":"en","@value":"The <I>in vitro</I> binding assay seems to be a useful first screening method for endocrine disrupting chemicals. The various methods have been developed and applied to the testing of chemicals. Although these assays should be applied to estrogen receptors (ER) of not only humans but also wildlife, a standardized system is yet to be established. Furthermore, a method for <I>Xenopus</I> ER is not yet developed. We previously expressed the ligand-binding domain (LBD) of quail ER<i>α</i> and ER<i>β</i> as a fusion protein with glutathione S-transferase, and developed a competitive enzyme immunoassay for detecting the capacity of chemicals to bind ERs. It seems that this system is a powerful tool, since it needs no special equipment. In this report, we first produced ER-LBD protein of human, <I>Xenopus</I> and medaka as well as quail. Then, we established a competitive enzyme immunoassay for these ERs as a standardized method, and compared the species specificity of the ability of 4-nonylphenol and <I>p</I>-octylphenol to bind ERs. Although a significant difference was not detected among ER<i>β</i> of human, quail and medaka, 4-nonylphenol and <I>p</I>-octylphenol exhibited the higher affinity for the medaka ER<i>α</i> than human ER<i>α</i>. These results indicate the species specificity of the capacity of chemicals to bind ERs. <br>"}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1420564276169396608","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"00363953"},{"@type":"NRID","@value":"1000000363953"},{"@type":"NRID","@value":"9000238429106"},{"@type":"NRID","@value":"9000363895815"},{"@type":"NRID","@value":"9000005133945"},{"@type":"NRID","@value":"9000021809976"},{"@type":"NRID","@value":"9000021944282"},{"@type":"NRID","@value":"9000020152219"},{"@type":"NRID","@value":"9000322102571"},{"@type":"NRID","@value":"9000005132450"},{"@type":"NRID","@value":"9000020031279"},{"@type":"NRID","@value":"9000020073139"},{"@type":"NRID","@value":"9000322102628"},{"@type":"NRID","@value":"9000020259013"},{"@type":"NRID","@value":"9000019043151"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0200441"}],"foaf:name":[{"@language":"en","@value":"Nishizuka Makoto"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Nagoya City University"}]},{"@id":"https://cir.nii.ac.jp/crid/1410001204497464064","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000005131882"}],"foaf:name":[{"@language":"en","@value":"Heitaku Shiro"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Nagoya City University"}]},{"@id":"https://cir.nii.ac.jp/crid/1410001204497464065","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000005131875"}],"foaf:name":[{"@language":"en","@value":"Maekawa Shinobu"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Nagoya City University"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282679474287235","@type":"Researcher","foaf:name":[{"@language":"en","@value":"Imagawa Masayoshi"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Nagoya City University"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282679474287236","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000005130728"}],"foaf:name":[{"@language":"en","@value":"Nishikawa Jun-ichi"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Laboratory of Environmental Biochemistry, Graduate School of Pharmaceutical Sciences, Osaka University"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"13449702"},{"@type":"EISSN","@value":"13475207"},{"@type":"CODEN","@value":"JHSCFD"},{"@type":"NDL_BIB_ID","@value":"000000160750"},{"@type":"ISSN","@value":"13449702"},{"@type":"LISSN","@value":"13449702"},{"@type":"NCID","@value":"AA11316464"}],"prism:publicationName":[{"@language":"ja","@value":"ＪＯＵＲＮＡＬ　ＯＦ　ＨＥＡＬＴＨ　ＳＣＩＥＮＣＥ"},{"@language":"en","@value":"Journal of Health Science"},{"@language":"en","@value":"JOURNAL OF HEALTH SCIENCE"},{"@language":"en","@value":"J. Health Sci."},{"@language":"ja","@value":"Ｊ．　Ｈｅａｌｔｈ　Ｓｃｉ．"}],"dc:publisher":[{"@language":"en","@value":"The Pharmaceutical Society of Japan"},{"@language":"ja","@value":"公益社団法人 日本薬学会"}],"prism:publicationDate":"2004","prism:volume":"50","prism:number":"5","prism:startingPage":"511","prism:endingPage":"517"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","url":[{"@id":"http://id.ndl.go.jp/bib/7097538"},{"@id":"https://ndlsearch.ndl.go.jp/books/R000000004-I7097538"},{"@id":"https://dl.ndl.go.jp/pid/8717535"},{"@id":"http://www.jstage.jst.go.jp/article/jhs/50/5/50_5_511/_pdf"},{"@id":"https://search.jamas.or.jp/link/ui/2006059976"}],"availableAt":"2004","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=endocrine%20disrupting%20chemicals","dc:title":"endocrine disrupting chemicals"},{"@id":"https://cir.nii.ac.jp/all?q=estrogen%20receptor","dc:title":"estrogen receptor"},{"@id":"https://cir.nii.ac.jp/all?q=enzyme%20immunoassay","dc:title":"enzyme immunoassay"},{"@id":"https://cir.nii.ac.jp/all?q=endocrine%20disruptor","dc:title":"endocrine disruptor"},{"@id":"https://cir.nii.ac.jp/all?q=%3CI%3Ein%20vitro%3C/I%3E%20binding%20assay","dc:title":"<I>in vitro</I> binding assay"},{"@id":"https://cir.nii.ac.jp/all?q=species%20specificity","dc:title":"species specificity"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360855570086269440","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Ecological risk assessment of endocrine disruptors."}]},{"@id":"https://cir.nii.ac.jp/crid/1360855570904780928","@type":"Article","relationType":["references","cites"],"jpcoar:relatedTitle":[{"@value":"New Screening Methods for Chemicals with Hormonal Activities Using Interaction of Nuclear Hormone Receptor with Coactivator"}]},{"@id":"https://cir.nii.ac.jp/crid/1362262944382935168","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Endocrine disrupters — testing strategies to assess human hazard"}]},{"@id":"https://cir.nii.ac.jp/crid/1362825894411752576","@type":"Article","relationType":["references","cites"],"jpcoar:relatedTitle":[{"@value":"Overview of endocrine disruptor research activity in the United States"}]},{"@id":"https://cir.nii.ac.jp/crid/1363107371188208512","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Nutritional implications of ginger: chemistry, biological activities and signaling pathways"}]},{"@id":"https://cir.nii.ac.jp/crid/1363388846214609024","@type":"Article","relationType":["references","cites"],"jpcoar:relatedTitle":[{"@value":"Endocrine Disruptors: Present Issues, Future Directions"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204497025280","@type":"Article","relationType":["references","cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Estrogenic Activities of 517 Chemicals by Yeast Two-Hybrid Assay."}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204497464192","@type":"Article","relationType":["references","cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Development of a Competitive Enzyme Immunoassay for Detection of Capacity of Chemicals to Bind Quail Estrogen Receptor .ALPHA. and .BETA."},{"@value":"Development of a Competitive Enzyme Immunoassay for Detection of Capacity of Chemicals to Bind Quail Estrogen Receptor α and β"},{"@language":"ja-Kana","@value":"Development of a Competitive Enzyme Immunoassay for Detection of Capacity of Chemicals to Bind Quail Estrogen Receptor アルファ and ベータ"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679475729536","@type":"Article","relationType":["isReferencedBy","isCitedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Evaluation of Ability of Chemicals to Bind Frog (<I>Xenopus laevis</I>) Estrogen Receptor by <I>in Vitro</I> Binding Assay"},{"@value":"Evaluation of Ability of Chemicals to Bind Frog (Xenopus laevis) Estrogen Receptor by in Vitro Binding Assay"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679942488192","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references","cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Basis of a High-Throughput Method for Nuclear Receptor Ligands"}]},{"@id":"https://cir.nii.ac.jp/crid/1390570397339896576","@type":"Article","relationType":["references","cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Endocrine disruptor issues in Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1570291225021410432","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Ecological risk assessment of endocrine disruptors"}]},{"@id":"https://cir.nii.ac.jp/crid/1571698601684790016","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Endocrine disrupters-testing strategies to assess human hazard. 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