{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1390282679485527552.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1252/kakoronbunshu.31.133"}},{"identifier":{"@type":"NDL_BIB_ID","@value":"7350243"}},{"identifier":{"@type":"URI","@value":"http://id.ndl.go.jp/bib/7350243"}},{"identifier":{"@type":"URI","@value":"https://ndlsearch.ndl.go.jp/books/R000000004-I7350243"}},{"identifier":{"@type":"URI","@value":"http://www.jstage.jst.go.jp/article/kakoronbunshu/31/2/31_2_133/_pdf"}},{"identifier":{"@type":"NAID","@value":"130000018649"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@language":"en","@value":"Characteristics of Hydrolysis with Lipase Immobilized in Mesoporous Silica"},{"@language":"ja","@value":"メソポーラスシリカ粒子に固定化したリパーゼによる加水分解反応特性"},{"@language":"ja-Kana","@value":"メソポーラスシリカ リュウシ ニ コテイカ シタ リパーゼ ニ ヨル カスイ ブンカイ ハンノウ トクセイ"}],"dc:language":"ja","description":[{"type":"abstract","notation":[{"@language":"en","@value":"Mesoporous silica powder (SBA15) with hexagonal pore structure (pore diameter, 7.5 nm and specific surface area, 584 m<sup>2</sup>/g) was prepared in the presence of tri-block copolymer as a template. Commercial mesoporous silica MCM41 with hexagonal pore structure (pore diameter, 2.9 nm and specific surface area, 759 m<sup>2</sup>/g) and SBA15 were used as a carrier for immobilization of a hydrolytic enzyme, lipase. SBA15 immobilized a greater amount of enzyme than MCM41 because of its larger pore diameter.<br>The hydrolysis of 2-naphthyl acetate with the lipase immobilized in mesoporous silica was examined at 25, 50 and 70°C by measuring the concentration of hydrolysis product, 2-naphthol. At 50 and 75°C, the immobilized lipase converted a greater proportion of 2-naphthyl acetate than the lipase in aqueous phase, and the conversion with MCM41 as carrier was greater than that with SBA15. On reuse of the immobilized lipase a conversion of more than 70% was obtained at 50°C. The thermal stability of lipase for the hydrolysis of 2-naphthyl acetate was increased by the immobilization of lipase in mesoporous silica."},{"@language":"ja","@value":"ブロック共重合体を鋳型として用いてヘキサゴナルの細孔構造を有する（細孔径7.5 nm，比表面積584 m<sup>2</sup>/g）メソポーラスシリカ粒子（SBA15）を調製した．SBA15およびヘキサゴナル構造を有する市販のMCM41（細孔径2.9 nm，比表面積759 m<sup>2</sup>/g）を加水分解酵素リパーゼの固定化担体として用いた．リパーゼの固定化量は，SBA15の方がMCM41より大きくなった．これらの固定化酵素による2-ナフチルアセテートの加水分解反応特性を生成物の2-ナフトール濃度の時間変化を25, 50および70°Cで測定して評価した．50°Cでは，リパーゼの固定化により転化率が増大した．高温での加水分解反応では，MCM41に固定化したリパーゼの転化率が，SBA15のそれよりも若干大きかった．また固定化リパーゼの再利用を検討した結果，50°Cでも70%以上の転化率を示した．メソポーラスシリカ粒子にリパーゼを固定化することによって，加水分解反応におけるリパーゼの酵素活性の熱安定性が増大した．"}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1410009221828778112","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000007023728"}],"foaf:name":[{"@language":"en","@value":"Maeda Satomi"},{"@language":"ja","@value":"前田 諭弥"}],"jpcoar:affiliationName":[{"@language":"ja","@value":"関西大学工学部 化学工学科"},{"@language":"en","@value":"Department of Chemical Engineering, Faculty of Engineering, Kansai University"}]},{"@id":"https://cir.nii.ac.jp/crid/1410009221828778113","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000007023729"}],"foaf:name":[{"@language":"en","@value":"Choshi Kenichi"},{"@language":"ja","@value":"調子 健一"}],"jpcoar:affiliationName":[{"@language":"ja","@value":"関西大学工学部 化学工学科"},{"@language":"en","@value":"Department of Chemical Engineering, Faculty of Engineering, Kansai University"}]},{"@id":"https://cir.nii.ac.jp/crid/1030003658373533952","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"70111995"},{"@type":"NRID","@value":"1000070111995"},{"@type":"NRID","@value":"9000021403173"},{"@type":"NRID","@value":"9000021493283"},{"@type":"NRID","@value":"9000020221449"},{"@type":"NRID","@value":"9000007024961"},{"@type":"NRID","@value":"9000020086499"},{"@type":"NRID","@value":"9000020212892"},{"@type":"NRID","@value":"9000000553963"},{"@type":"NRID","@value":"9000007027660"},{"@type":"NRID","@value":"9000020707748"},{"@type":"NRID","@value":"9000000555940"},{"@type":"NRID","@value":"9000016386733"},{"@type":"NRID","@value":"9000020050073"},{"@type":"NRID","@value":"9000020226740"},{"@type":"NRID","@value":"9000020673285"},{"@type":"NRID","@value":"9000238245835"},{"@type":"NRID","@value":"9000007028167"},{"@type":"NRID","@value":"9000022035799"},{"@type":"NRID","@value":"9000020788077"},{"@type":"NRID","@value":"9000254254227"},{"@type":"NRID","@value":"9000254254218"},{"@type":"NRID","@value":"9000021982969"},{"@type":"NRID","@value":"9000020781690"},{"@type":"NRID","@value":"9000020150783"},{"@type":"NRID","@value":"9000356516448"},{"@type":"NRID","@value":"9000253479724"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0013564"}],"foaf:name":[{"@language":"ja","@value":"三宅 義和"},{"@language":"en","@value":"Miyake Yoshikazu"}],"jpcoar:affiliationName":[{"@language":"ja","@value":"関西大学工学部 化学工学科"},{"@language":"en","@value":"Department of Chemical Engineering, Faculty of Engineering, Kansai University"},{"@language":"en","@value":"High Technology Research Center, Kansai University"},{"@language":"ja","@value":"関西大学ハイテクリサーチセンター"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"0386216X"},{"@type":"LISSN","@value":"0386216X"},{"@type":"EISSN","@value":"13499203"},{"@type":"NDL_BIB_ID","@value":"000000026852"},{"@type":"ISSN","@value":"0386216X"},{"@type":"NCID","@value":"AN00037234"}],"prism:publicationName":[{"@language":"en","@value":"KAGAKU KOGAKU RONBUNSHU"},{"@language":"ja","@value":"化学工学論文集"},{"@language":"en","@value":"KAGAKU KOGAKU RONBUNSHU"},{"@language":"ja","@value":"化学工学論文集"}],"dc:publisher":[{"@language":"en","@value":"The Society of Chemical Engineers, Japan"},{"@language":"ja","@value":"公益社団法人 化学工学会"}],"prism:publicationDate":"2005","prism:volume":"31","prism:number":"2","prism:startingPage":"133","prism:endingPage":"137"},"reviewed":"false","url":[{"@id":"http://id.ndl.go.jp/bib/7350243"},{"@id":"https://ndlsearch.ndl.go.jp/books/R000000004-I7350243"},{"@id":"http://www.jstage.jst.go.jp/article/kakoronbunshu/31/2/31_2_133/_pdf"}],"availableAt":"2005","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Mesoporous%20Silica","dc:title":"Mesoporous Silica"},{"@id":"https://cir.nii.ac.jp/all?q=Lipase","dc:title":"Lipase"},{"@id":"https://cir.nii.ac.jp/all?q=Immobilized%20Enzyme","dc:title":"Immobilized Enzyme"},{"@id":"https://cir.nii.ac.jp/all?q=Hydrolysis","dc:title":"Hydrolysis"},{"@id":"https://cir.nii.ac.jp/all?q=Template","dc:title":"Template"},{"@id":"https://cir.nii.ac.jp/all?q=%E3%83%A1%E3%82%BD%E3%83%9D%E3%83%BC%E3%83%A9%E3%82%B9%E3%82%B7%E3%83%AA%E3%82%AB","dc:title":"メソポーラスシリカ"},{"@id":"https://cir.nii.ac.jp/all?q=%E3%83%AA%E3%83%91%E3%83%BC%E3%82%BC","dc:title":"リパーゼ"},{"@id":"https://cir.nii.ac.jp/all?q=%E5%9B%BA%E5%AE%9A%E5%8C%96%E9%85%B5%E7%B4%A0","dc:title":"固定化酵素"},{"@id":"https://cir.nii.ac.jp/all?q=%E5%8A%A0%E6%B0%B4%E5%88%86%E8%A7%A3","dc:title":"加水分解"},{"@id":"https://cir.nii.ac.jp/all?q=%E9%8B%B3%E5%9E%8B","dc:title":"鋳型"}],"project":[{"@id":"https://cir.nii.ac.jp/crid/1040282256859881088","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"16560662"},{"@type":"JGN","@value":"JP16560662"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16560662/"}],"notation":[{"@language":"ja","@value":"逆ヘキサゴナル構造を有するメソポーラスシリカを用いた分離材料の開発"},{"@language":"en","@value":"Development of Mesoporous Silica Particles with Reversed Hexagonal Nano-structure used as Separation Materials"}]}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360011143914866816","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Hexagonal to Mesocellular Foam Phase Transition in Polymer-Templated Mesoporous Silicas"}]},{"@id":"https://cir.nii.ac.jp/crid/1360292619462256384","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Encapsulation of enzymes in mesoporous host materials via the nonsurfactant-templated sol–gel process"}]},{"@id":"https://cir.nii.ac.jp/crid/1361699996254367616","@type":"Article","relationType":["references","cites"],"jpcoar:relatedTitle":[{"@value":"Triblock Copolymer Syntheses of Mesoporous Silica with Periodic 50 to 300 Angstrom Pores"}]},{"@id":"https://cir.nii.ac.jp/crid/1361981471460122368","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Separation of biological 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