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The MCR-F430 complex catalyzes the last step of methanogenesis: reduction of methyl-coenzyme M to methane. Since F430 is a common enzyme for mathanogens, it can be a function-specific biomarker to estimate biomass and activities of methanogens in environmental samples. A recently developed high sensitive method for coenzyme F430 analysis and a further purification step revealed F430 concentrations at 69 and 88 meters below seafloor (mbsf) off Shimokita Peninsula (529 and 31.3 fmol g-wet<sup>–1</sup>) and at 60 and 275 mbsf at Nankai Trough (31.4 and 26 fmol g-wet<sup>–1</sup>). Estimated methanogenic biomass and activities based on reported F430 content and activities in methanogens and prokaryotic cell weight, were ranging from 3.9 × 10<sup>6</sup> to 2.7 × 10<sup>6</sup> cells g-wet<sup>–1</sup> and from 1.7 to 11 μmol d<sup>–1</sup> g-wet<sup>–1</sup>.</p>"}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1420564276189792896","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"80633239"},{"@type":"NRID","@value":"1000080633239"},{"@type":"NRID","@value":"9000003938937"},{"@type":"NRID","@value":"9000003899594"},{"@type":"NRID","@value":"9000347188789"},{"@type":"NRID","@value":"9000006356559"},{"@type":"NRID","@value":"9000239624528"},{"@type":"NRID","@value":"9000021261607"},{"@type":"NRID","@value":"9000240032365"},{"@type":"NRID","@value":"9000258405069"},{"@type":"NRID","@value":"9000331897013"},{"@type":"NRID","@value":"9000239625432"},{"@type":"NRID","@value":"9000283904150"},{"@type":"NRID","@value":"9000345248826"},{"@type":"NRID","@value":"9000283595412"},{"@type":"NRID","@value":"9000315142735"},{"@type":"NRID","@value":"9000017552853"},{"@type":"NRID","@value":"9000331895542"},{"@type":"NRID","@value":"9000264238158"},{"@type":"NRID","@value":"9000345404532"},{"@type":"NRID","@value":"9000398987584"},{"@type":"NRID","@value":"9000018751070"},{"@type":"NRID","@value":"9000283905727"},{"@type":"NRID","@value":"9000305639662"},{"@type":"NRID","@value":"9000258407616"},{"@type":"NRID","@value":"9000290821392"},{"@type":"NRID","@value":"9000252823837"},{"@type":"NRID","@value":"9000370281251"},{"@type":"NRID","@value":"9000317563801"},{"@type":"NRID","@value":"9000345439012"},{"@type":"NRID","@value":"9000239624420"},{"@type":"NRID","@value":"9000239624537"},{"@type":"NRID","@value":"9000264238181"},{"@type":"NRID","@value":"9000402477216"},{"@type":"NRID","@value":"9000239624709"},{"@type":"NRID","@value":"9000258405400"},{"@type":"NRID","@value":"9000239624556"},{"@type":"NRID","@value":"9000021324179"},{"@type":"NRID","@value":"9000317155251"},{"@type":"NRID","@value":"9000402477270"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/masanorikaneko"}],"foaf:name":[{"@language":"en","@value":"Kaneko 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Naohiko"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Japan Agency for Marine-Earth Science and Technology"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00167002"},{"@type":"LISSN","@value":"00167002"},{"@type":"EISSN","@value":"18805973"},{"@type":"NDL_BIB_ID","@value":"000000128646"},{"@type":"ISSN","@value":"00167002"},{"@type":"NCID","@value":"AA00654975"}],"prism:publicationName":[{"@language":"en","@value":"GEOCHEMICAL JOURNAL"},{"@language":"ja","@value":"ＧＥＯＣＨＥＭＩＣＡＬ　ＪＯＵＲＮＡＬ"},{"@language":"en","@value":"Geochem. J."},{"@language":"ja","@value":"Ｇｅｏｃｈｅｍ．　Ｊ．"}],"dc:publisher":[{"@language":"en","@value":"GEOCHEMICAL SOCIETY OF JAPAN"},{"@language":"ja","@value":"一般社団法人日本地球化学会"}],"prism:publicationDate":"2016","prism:volume":"50","prism:number":"6","prism:startingPage":"453","prism:endingPage":"460"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","url":[{"@id":"http://id.ndl.go.jp/bib/027766708"},{"@id":"https://ndlsearch.ndl.go.jp/books/R000000004-I027766708"},{"@id":"https://www.jstage.jst.go.jp/article/geochemj/50/6/50_2.0410/_pdf"}],"availableAt":"2016","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=coenzyme%20F430","dc:title":"coenzyme F430"},{"@id":"https://cir.nii.ac.jp/all?q=methane%20hydrate","dc:title":"methane hydrate"},{"@id":"https://cir.nii.ac.jp/all?q=methanogens","dc:title":"methanogens"},{"@id":"https://cir.nii.ac.jp/all?q=function-specific%20compound%20analysis","dc:title":"function-specific compound analysis"},{"@id":"https://cir.nii.ac.jp/all?q=marine%20sediment","dc:title":"marine sediment"}],"project":[{"@id":"https://cir.nii.ac.jp/crid/1040000781833166464","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"15H05332"},{"@type":"JGN","@value":"JP15H05332"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15H05332/"}],"notation":[{"@language":"ja","@value":"光合成とメタン生成のリンケージ：機能特異分子補酵素F430分析という新手法の展開"},{"@language":"en","@value":"Linkages between photosynthesis and methanogenesis: application of function specific compound analysis of coenzyme F430"}]},{"@id":"https://cir.nii.ac.jp/crid/1040000782266029312","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"25610166"},{"@type":"JGN","@value":"JP25610166"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-25610166/"}],"notation":[{"@language":"ja","@value":"機能性分子F430を用いた堆積物深部メタン生成ポテンシャルの精密定量"},{"@language":"en","@value":"Quantification of methanogenic potential 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