書誌事項
- タイトル別名
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- On the Sugar Composition of Hydrol from the Enzymatic Hydrolyzate of Starch Part II
- デンプン コウソ トウカ ハイドロール ノ トウ ソセイ ニ ツイテ 2 コウソザイ ニ ヨル ギャクゴウセイ トウルイ ノ ケントウ
- Reversion Product from D-Glucose by Crude Glucoamylase
- 酵素剤による逆合成糖類の検討
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説明
Product of reverse action of glucoamylase was prepared by incubating 40% D-glucose solution with 0. 5% of crude glucoamylase at 55°C for 72_??_96 hrs, followed by inactivating the enzyme at 75°C for 10 min. By paper chromatography and paper ionophoresis, nigerose, maltose, isomaltose, laminaribiose, gentiobiose, panose, isopanose and isomaltotriose were detected in the reaction product. Reversion product from 650g of D-glucose was fractionated by a Carbon-Celite column using water and 25_??_15% ethanol as successive elution solvents. Further separation of sugar was carried out by thick paper chromatography and also by Magnesol-Celite column chromatography of the acetylated sugars. In this way, isomaltose, nigerose, maltose, gentiobiose and laminaribiose were identified as their crystalline octaacetates. Panose was identified as crystalline panitol dodecaacetate.<br> These results suggest that the glucobioses and glucotrioses in the hydrol were produced from D-glucose by the reverse action of crude enzyme. The enzyme used in this experiment showed no transglucosylase activity.
収録刊行物
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- 日本農芸化学会誌
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日本農芸化学会誌 40 (11), 401-405, 1966
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679539180288
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- NII論文ID
- 130001222376
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- NII書誌ID
- AN00196191
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- ISSN
- 18836844
- 00021407
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- NDL書誌ID
- 8399505
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可