{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1390282679601579264.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1248/bpb.31.2154"}},{"identifier":{"@type":"NDL_BIB_ID","@value":"9685850"}},{"identifier":{"@type":"URI","@value":"http://id.ndl.go.jp/bib/9685850"}},{"identifier":{"@type":"URI","@value":"https://ndlsearch.ndl.go.jp/books/R000000004-I9685850"}},{"identifier":{"@type":"URI","@value":"http://www.jstage.jst.go.jp/article/bpb/31/11/31_11_2154/_pdf"}},{"identifier":{"@type":"NAID","@value":"110006968105"}},{"identifier":{"@type":"URI","@value":"https://search.jamas.or.jp/link/ui/2009092907"}}],"dc:title":[{"@language":"en","@value":"Anti-diabetic Properties of Chrysophanol and Its Glucoside from Rhubarb Rhizome"}],"dc:language":"en","description":[{"type":"abstract","notation":[{"@language":"en","@value":"An ethanol extract of rhubarb rhizome exhibited marked glucose transport activity in differentiated L6 rat myotubes. Activity-guided fractionation resulted in the isolation of two anthraquinones, chrysophanol-8-<i>O</i>-β-<small>D</small>-glucopyranoside (1) and chrysophanol (2). The anti-diabetic effect was examined by glucose transport activity, glucose transporter 4 (Glut4) expression in myotubes, and the level of insulin receptor (IR) tyrosine phosphorylation as influenced by tyrosine phosphatase 1B, each of which is a major target of diabetes treatment. Chrysophanol-8-<i>O</i>-β-<small>D</small>-glucopyranoside up to 25 μ<small>M</small> dose-dependently activated glucose transport in insulin-stimulated myotubes. Increased tyrosine phosphorylation of IR due to tyrosine phosphatase 1B inhibitory activity with an IC<sub>50</sub> value of 18.34±0.29 μ<small>M</small> and unchanged Glut4 mRNA levels was observed following chrysophanol-8-<i>O</i>-β-<small>D</small>-glucopyranoside treatment. Chrysophanol up to 100 μ<small>M</small> exerted mild glucose transport activity and elevated the tyrosine phosphorylation of IR <i>via</i> tyrosine phosphatase 1B inhibition (IC<sub>50</sub>=79.86±0.12 μ<small>M</small>); Glut4 mRNA expression was also significantly increased by 100 μ<small>M</small>. The ED<sub>50</sub> values of the two compounds were 59.38±0.66 and 79.69±0.03 μ<small>M</small>, respectively. Therefore, these two anthraquinones from rhubarb rhizome, chrysophanol-8-<i>O</i>-β-<small>D</small>-glucopyranoside and chrysophanol, have mild cytotoxicity and anti-diabetic properties and could play metabolic roles in the insulin-stimulated glucose transport pathway."}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1410001204148992903","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000006834560"},{"@type":"NRID","@value":"9000252790966"}],"foaf:name":[{"@language":"en","@value":"Lee Myung Sun"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Food and Nutrition, Chungnam National University"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282679601579264","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000006834563"}],"foaf:name":[{"@language":"en","@value":"Sohn Cheon Bae"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Food and Nutrition, Chungnam National University"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"09186158"},{"@type":"EISSN","@value":"13475215"},{"@type":"NDL_BIB_ID","@value":"000000153250"},{"@type":"ISSN","@value":"09186158"},{"@type":"LISSN","@value":"09186158"},{"@type":"NCID","@value":"AA10885497"}],"prism:publicationName":[{"@language":"ja","@value":"Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ"},{"@language":"en","@value":"Biological and Pharmaceutical Bulletin"},{"@language":"en","@value":"Biological & Pharmaceutical Bulletin"},{"@language":"en","@value":"Biol. Pharm. Bull."},{"@language":"ja","@value":"Ｂｉｏｌ．　Ｐｈａｒｍ．　Ｂｕｌｌ．"}],"dc:publisher":[{"@language":"en","@value":"The Pharmaceutical Society of Japan"},{"@language":"ja","@value":"公益社団法人 日本薬学会"}],"prism:publicationDate":"2008","prism:volume":"31","prism:number":"11","prism:startingPage":"2154","prism:endingPage":"2157"},"reviewed":"false","url":[{"@id":"http://id.ndl.go.jp/bib/9685850"},{"@id":"https://ndlsearch.ndl.go.jp/books/R000000004-I9685850"},{"@id":"http://www.jstage.jst.go.jp/article/bpb/31/11/31_11_2154/_pdf"},{"@id":"https://search.jamas.or.jp/link/ui/2009092907"}],"availableAt":"2008","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=rhubarb","dc:title":"rhubarb"},{"@id":"https://cir.nii.ac.jp/all?q=anthraquinone","dc:title":"anthraquinone"},{"@id":"https://cir.nii.ac.jp/all?q=diabetes","dc:title":"diabetes"},{"@id":"https://cir.nii.ac.jp/all?q=glucose%20transport","dc:title":"glucose 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