Direct Cytotoxicity of Lentinula edodes Mycelia Extract on Human Hepatocellular Carcinoma Cell Line
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- Tomi Hironori
- Department of New Product and Business Development, Central R&D Laboratory, Kobayashi Pharmaceutical Co., Ltd.
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- Yukawa Hiroshi
- Department of New Product and Business Development, Central R&D Laboratory, Kobayashi Pharmaceutical Co., Ltd.
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- Kawanishi Takashi
- Department of New Product and Business Development, Central R&D Laboratory, Kobayashi Pharmaceutical Co., Ltd.
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- Ishikawa Satoru
- Department of New Product and Business Development, Central R&D Laboratory, Kobayashi Pharmaceutical Co., Ltd.
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- Tamesada Makoto
- Department of New Product and Business Development, Central R&D Laboratory, Kobayashi Pharmaceutical Co., Ltd.
書誌事項
- タイトル別名
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- Direct Cytotoxicity of <i>Lentinula edodes</i> Mycelia Extract on Human Hepatocellular Carcinoma Cell Line
- 公開日
- 2012
- 資源種別
- journal article
- DOI
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- 10.1248/bpb.b110657
- 公開者
- 公益社団法人 日本薬学会
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説明
Lentinula edodes mycelia (L.E.M.) is a dried powder extracted from shiitake mushrooms (Lentinula edodes). We previously demonstrated that it has immunomodulatory effects. In this paper, the direct cytotoxic effects of the polysaccharide-rich fraction of L.E.M. (L.E.M. ethanol precipitate; LEP) on HepG2 human hepatocellular carcinoma (HCC) cells were investigated. LEP directly killed the HepG2 cells efficaciously, but had only minor effects on normal rat hepatocytes and normal mouse dermal cells under the same conditions. Characteristic morphological changes associated with apoptosis such as shrinkage, rounding, and floating as well as chromatin condensation were confirmed; terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) staining was positive as determined by fluorescence microscopy analyses. The caspase-3 and -8 death receptor pathway was found largely responsible for the apoptotic death of HepG2 cells treated with LEP. In conclusion, LEP can directly induce apoptosis of HepG2 cells, and thus may have potential chemotherapeutic applications for the treatment of HCC.
収録刊行物
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- Ｂｉｏｌｏｇｉｃａｌ ＆ Ｐｈａｒｍａｃｅｕｔｉｃａｌ Ｂｕｌｌｅｔｉｎ
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Ｂｉｏｌｏｇｉｃａｌ ＆ Ｐｈａｒｍａｃｅｕｔｉｃａｌ Ｂｕｌｌｅｔｉｎ 35 (7), 1014-1021, 2012
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390282679609010048
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- NII論文ID
- 130001872102
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- NII書誌ID
- AA10885497
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- COI
- 1:STN:280:DC%2BC38jps1Ogtg%3D%3D
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 023765471
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- PubMed
- 22791146
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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- JaLC
- NDLサーチ
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可
