{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1390282679609010048.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1248/bpb.b110657"}},{"identifier":{"@type":"COI","@value":"1:STN:280:DC%2BC38jps1Ogtg%3D%3D"}},{"identifier":{"@type":"PMID","@value":"22791146"}},{"identifier":{"@type":"NDL_BIB_ID","@value":"023765471"}},{"identifier":{"@type":"URI","@value":"http://id.ndl.go.jp/bib/023765471"}},{"identifier":{"@type":"URI","@value":"https://ndlsearch.ndl.go.jp/books/R000000004-I023765471"}},{"identifier":{"@type":"URI","@value":"https://www.jstage.jst.go.jp/article/bpb/35/7/35_b110657/_pdf"}},{"identifier":{"@type":"NAID","@value":"130001872102"}},{"identifier":{"@type":"URI","@value":"https://search.jamas.or.jp/link/ui/2013156778"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@language":"en","@value":"Direct Cytotoxicity of <i>Lentinula edodes</i> Mycelia Extract on Human Hepatocellular Carcinoma Cell Line"},{"@value":"Direct Cytotoxicity of Lentinula edodes Mycelia Extract on Human Hepatocellular Carcinoma Cell Line"}],"dc:language":"en","description":[{"type":"abstract","notation":[{"@language":"en","@value":"<i>Lentinula edodes</i> mycelia (L.E.M.) is a dried powder extracted from shiitake mushrooms (<i>Lentinula edodes</i>). We previously demonstrated that it has immunomodulatory effects. In this paper, the direct cytotoxic effects of the polysaccharide-rich fraction of L.E.M. (L.E.M. ethanol precipitate; LEP) on HepG2 human hepatocellular carcinoma (HCC) cells were investigated. LEP directly killed the HepG2 cells efficaciously, but had only minor effects on normal rat hepatocytes and normal mouse dermal cells under the same conditions. Characteristic morphological changes associated with apoptosis such as shrinkage, rounding, and floating as well as chromatin condensation were confirmed; terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) staining was positive as determined by fluorescence microscopy analyses. The caspase-3 and -8 death receptor pathway was found largely responsible for the apoptotic death of HepG2 cells treated with LEP. In conclusion, LEP can directly induce apoptosis of HepG2 cells, and thus may have potential chemotherapeutic applications for the treatment of HCC."}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1410282679609010051","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000021818303"}],"foaf:name":[{"@language":"en","@value":"Tomi Hironori"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of New Product and Business Development, Central R&D Laboratory, Kobayashi Pharmaceutical Co., Ltd."}]},{"@id":"https://cir.nii.ac.jp/crid/1420282801196425856","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"30634646"},{"@type":"NRID","@value":"1000030634646"},{"@type":"NRID","@value":"9000409902305"},{"@type":"NRID","@value":"9000006449697"},{"@type":"NRID","@value":"9000021818273"},{"@type":"NRID","@value":"9000365539533"},{"@type":"NRID","@value":"9000413249488"},{"@type":"NRID","@value":"9000402049413"},{"@type":"NRID","@value":"9000404505436"},{"@type":"NRID","@value":"9000345348100"},{"@type":"NRID","@value":"9000405887800"},{"@type":"NRID","@value":"9000283134049"},{"@type":"NRID","@value":"9000411813990"},{"@type":"NRID","@value":"9000318587660"},{"@type":"NRID","@value":"9000405889026"},{"@type":"NRID","@value":"9000273030107"},{"@type":"NRID","@value":"9000346962775"},{"@type":"NRID","@value":"9000389942876"},{"@type":"NRID","@value":"9000345348095"},{"@type":"NRID","@value":"9000326264741"},{"@type":"NRID","@value":"9000405887789"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/h.yukawa_NQ"}],"foaf:name":[{"@language":"en","@value":"Yukawa Hiroshi"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of New Product and Business Development, Central R&D Laboratory, Kobayashi Pharmaceutical Co., Ltd."}]},{"@id":"https://cir.nii.ac.jp/crid/1410282679609010050","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000021818288"}],"foaf:name":[{"@language":"en","@value":"Kawanishi Takashi"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of New Product and Business Development, Central R&D Laboratory, Kobayashi Pharmaceutical Co., Ltd."}]},{"@id":"https://cir.nii.ac.jp/crid/1410282679609010048","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000021818283"}],"foaf:name":[{"@language":"en","@value":"Ishikawa Satoru"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of New Product and Business Development, Central R&D Laboratory, Kobayashi Pharmaceutical Co., Ltd."}]},{"@id":"https://cir.nii.ac.jp/crid/1410282679609010052","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000021818295"}],"foaf:name":[{"@language":"en","@value":"Tamesada Makoto"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of New Product and Business Development, Central R&D Laboratory, Kobayashi Pharmaceutical Co., Ltd."}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"09186158"},{"@type":"EISSN","@value":"13475215"},{"@type":"NDL_BIB_ID","@value":"000000153250"},{"@type":"ISSN","@value":"09186158"},{"@type":"LISSN","@value":"09186158"},{"@type":"NCID","@value":"AA10885497"}],"prism:publicationName":[{"@language":"ja","@value":"Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ"},{"@language":"en","@value":"Biological and Pharmaceutical Bulletin"},{"@language":"en","@value":"Biological & Pharmaceutical Bulletin"},{"@language":"en","@value":"Biol. Pharm. Bull."},{"@language":"ja","@value":"Ｂｉｏｌ．　Ｐｈａｒｍ．　Ｂｕｌｌ．"}],"dc:publisher":[{"@language":"en","@value":"The Pharmaceutical Society of Japan"},{"@language":"ja","@value":"公益社団法人 日本薬学会"}],"prism:publicationDate":"2012","prism:volume":"35","prism:number":"7","prism:startingPage":"1014","prism:endingPage":"1021"},"reviewed":"false","url":[{"@id":"http://id.ndl.go.jp/bib/023765471"},{"@id":"https://ndlsearch.ndl.go.jp/books/R000000004-I023765471"},{"@id":"https://www.jstage.jst.go.jp/article/bpb/35/7/35_b110657/_pdf"},{"@id":"https://search.jamas.or.jp/link/ui/2013156778"}],"availableAt":"2012","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=%3Ci%3ELentinula%20edodes%3C/i%3E%20mycelia","dc:title":"<i>Lentinula edodes</i> mycelia"},{"@id":"https://cir.nii.ac.jp/all?q=hepatocellular%20carcinom","dc:title":"hepatocellular carcinom"},{"@id":"https://cir.nii.ac.jp/all?q=apoptosis","dc:title":"apoptosis"},{"@id":"https://cir.nii.ac.jp/all?q=caspase","dc:title":"caspase"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360011143632743040","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Nod1, an Apaf-1-like Activator of Caspase-9 and Nuclear Factor-κB"}]},{"@id":"https://cir.nii.ac.jp/crid/1360283691134675200","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Tissue invasion and metastasis: Molecular, biological and clinical perspectives"}]},{"@id":"https://cir.nii.ac.jp/crid/1360292620481804544","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Induction of apoptosis in human hepatoma cells by mycelia of Antrodia camphorata in submerged 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Effect of Syringic Acid and Vanillic Acid on CCl4-Induced Liver Injury"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204628025984","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Protection against D-Galactosamine-Induced Acute Liver Injury by Oral Administration of Extracts from Lentinus edodes Mycelia"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679604054272","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Hepatoprotective Effect of Syringic Acid and Vanillic Acid on Concanavalin A-Induced Liver 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