{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1390282679617697280.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.2745/dds.18.361"}},{"identifier":{"@type":"NAID","@value":"10011212506"}},{"identifier":{"@type":"URI","@value":"https://search.jamas.or.jp/link/ui/2003291178"}}],"dc:title":[{"@language":"en","@value":"Autotaxin has lysophospholipase D activity leading to tumor cell growth and motility by lysophosphatidic acid production"},{"@value":"抗がん剤のｍｏｌｅｃｕｌａｒ　ｔａｒｇｅｔｉｎｇ　　細胞浸潤・増殖因子"}],"dcterms:alternative":[{"@language":"en","@value":"Autotaxin has lysophospholipase D activity leading to tumor cell growth and motility by lysophosplealidic acid production"},{"@value":"細胞浸潤·増殖因子"}],"dc:language":"ja","description":[{"type":"abstract","notation":[{"@language":"en","@value":"Autotaxin (ATX) is a tumor cell motility-stimulating factor, originally isolated from melanoma cell supernatants. ATX had been proposed to mediate its effects through 5'-nucleotide pyrophosphatase and phosphodiesterase activities. However, the ATX substrate mediating the increase in cellular motility remains to he identified. Here, we demonstrated that lysophospholipase D (lysoPLD) purified from fetal bovine serum, which catalyzes the production of the bioactive phospholipid mediator, lysophosphatidic acid (LPA), from lysophosphatidylcholine(LPC), is identical to ATX. The Km value of ATX for LPC was 25-fold lower than that for the synthetic nucleoside substrate, p-nitrophenyl-tri-monophosphate. LPA mediates multiple biological functions including cytoskeletal reorganization, chemotaxis and cell growth through activation of specific G-protein-coupled receptors. Recombinant ATX, particularly in the presence of LPC, dramatically increased chemotaxis and proliferation of multiple different cell lines. Moreover, we demonstrate that several cancer cell lines release significant amounts of LPC, a substrate for ATX, into the culture medium. The demonstration that ATX and lysoPLD are identical suggests that autocrine or paracrine production of LPA contributes to tumor cell motility, survival and proliferation. It also provides potential novel targets for therapy of pathophysiological states, including cancer."}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1420282801194736640","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"20250219"},{"@type":"NRID","@value":"1000020250219"},{"@type":"ORCID","@value":"0000-0001-9435-1896"},{"@type":"NRID","@value":"9000000417872"},{"@type":"NRID","@value":"9000017289720"},{"@type":"NRID","@value":"9000411795906"},{"@type":"NRID","@value":"9000327093486"},{"@type":"NRID","@value":"9000252814570"},{"@type":"NRID","@value":"9000261043561"},{"@type":"NRID","@value":"9000409849646"},{"@type":"NRID","@value":"9000414502880"},{"@type":"NRID","@value":"9000253171807"},{"@type":"NRID","@value":"9000399220217"},{"@type":"NRID","@value":"9000283649572"},{"@type":"NRID","@value":"9000356514034"},{"@type":"NRID","@value":"9000391611375"},{"@type":"NRID","@value":"9000252815086"},{"@type":"NRID","@value":"9000397946300"},{"@type":"NRID","@value":"9000404829743"},{"@type":"NRID","@value":"9000399009768"},{"@type":"NRID","@value":"9000017331148"},{"@type":"NRID","@value":"9000021994811"},{"@type":"NRID","@value":"9000365542158"},{"@type":"NRID","@value":"9000402771868"},{"@type":"NRID","@value":"9000016590108"},{"@type":"NRID","@value":"9000018350195"},{"@type":"NRID","@value":"9000402125275"},{"@type":"NRID","@value":"9000020455109"},{"@type":"NRID","@value":"9000020455315"},{"@type":"NRID","@value":"9000241474646"},{"@type":"NRID","@value":"9000264868566"},{"@type":"NRID","@value":"9000412400895"},{"@type":"NRID","@value":"9000314382970"},{"@type":"NRID","@value":"9000411643374"},{"@type":"NRID","@value":"9000403260250"},{"@type":"NRID","@value":"9000272991585"},{"@type":"NRID","@value":"9000398998176"},{"@type":"NRID","@value":"9000018562022"},{"@type":"NRID","@value":"9000390892292"},{"@type":"NRID","@value":"9000362192773"},{"@type":"NRID","@value":"9000398397116"},{"@type":"NRID","@value":"9000018234758"},{"@type":"NRID","@value":"9000411784322"},{"@type":"NRID","@value":"9000399764650"},{"@type":"NRID","@value":"9000331092472"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/junken_aoki"}],"foaf:name":[{"@language":"en","@value":"Aoki Junken"},{"@language":"ja","@value":"青木 淳賢"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Graduate School of Pharmaceutical Sciences University of Tokyo"},{"@language":"ja","@value":"東京大学大学院薬学系研究科衛生化学教室"}]},{"@id":"https://cir.nii.ac.jp/crid/1410853646632655232","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000001030540"}],"foaf:name":[{"@language":"en","@value":"Kishi Yasuhiro"},{"@language":"ja","@value":"岸 安宏"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Graduate School of Pharmaceutical Sciences University of Tokyo"},{"@language":"ja","@value":"東京大学大学院薬学系研究科衛生化学教室"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"09135006"},{"@type":"EISSN","@value":"18812732"},{"@type":"CODEN","@value":"DDSYEI"},{"@type":"NCID","@value":"AN10084591"}],"prism:publicationName":[{"@language":"en","@value":"Drug Delivery System"},{"@language":"ja","@value":"Ｄｒｕｇ　Ｄｅｌｉｖｅｒｙ　Ｓｙｓｔｅｍ"},{"@language":"en","@value":"DDS"},{"@language":"en","@value":"Official Journal of the Japan Society of Drug Delivery System"},{"@language":"ja","@value":"ＤＤＳ"}],"dc:publisher":[{"@language":"en","@value":"THE JAPAN SOCIETY OF DRUG DELIVERY SYSTEM"},{"@language":"ja","@value":"日本DDS学会"}],"prism:publicationDate":"2003","prism:volume":"18","prism:number":"4","prism:startingPage":"361","prism:endingPage":"366"},"reviewed":"false","url":[{"@id":"https://search.jamas.or.jp/link/ui/2003291178"}],"availableAt":"2003","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=lysophosphatidic%20acid","dc:title":"lysophosphatidic acid"},{"@id":"https://cir.nii.ac.jp/all?q=autotaxin","dc:title":"autotaxin"},{"@id":"https://cir.nii.ac.jp/all?q=lysoPLD","dc:title":"lysoPLD"},{"@id":"https://cir.nii.ac.jp/all?q=metastasis/invasion","dc:title":"metastasis/invasion"},{"@id":"https://cir.nii.ac.jp/all?q=cancer","dc:title":"cancer"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360292619606245888","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@value":"Phenotypic Modulation of Vascular Smooth Muscle Cells Induced by Unsaturated Lysophosphatidic Acids"}]},{"@id":"https://cir.nii.ac.jp/crid/1360855568830000128","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@value":"A family of phospholipid autacoids: Occurrence, metabolism and bioactions"}]},{"@id":"https://cir.nii.ac.jp/crid/1361699994262218112","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@value":"Affinity Purification and cDNA Cloning of Rat Neural Differentiation and Tumor Cell Surface Antigen gp130RB13-6 Reveals Relationship to Human and Murine PC-1"}]},{"@id":"https://cir.nii.ac.jp/crid/1361981470284943104","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@value":"Autotaxin Is an Exoenzyme Possessing 5′-Nucleotide Phosphodiesterase/ATP Pyrophosphatase and ATPase Activities"}]},{"@id":"https://cir.nii.ac.jp/crid/1362262944023141248","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@value":"Characterization of <i>lpa<sub>2</sub></i> (<i>Edg4</i>) and <i>lpa<sub>1</sub></i>/<i>lpa<sub>2</sub></i> (<i>Edg2/Edg4</i>) Lysophosphatidic Acid Receptor Knockout Mice: Signaling Deficits without Obvious Phenotypic Abnormality Attributable to <i>lpa<sub>2</sub></i>"}]},{"@id":"https://cir.nii.ac.jp/crid/1362544419001280512","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@value":"SURFACE ALLOANTIGENS OF PLASMA CELLS"}]},{"@id":"https://cir.nii.ac.jp/crid/1362825896014984064","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@value":"Serum Lysophosphatidic Acid Is Produced through Diverse Phospholipase Pathways"}]},{"@id":"https://cir.nii.ac.jp/crid/1363107368435734784","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@value":"Autotaxin has lysophospholipase D activity leading to tumor cell growth and motility by lysophosphatidic acid production"}]},{"@id":"https://cir.nii.ac.jp/crid/1363107370829559936","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@value":"Autotaxin, tumor motility-stimulating exophosphodiesterase"}]},{"@id":"https://cir.nii.ac.jp/crid/1363388844617243136","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@value":"Requirement for the\n            <i>lp</i>\n            <sub>A1</sub>\n            lysophosphatidic acid receptor gene in normal suckling behavior"}]},{"@id":"https://cir.nii.ac.jp/crid/1570009750001436288","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Insulin-like growth factor II acts as an autocrine growth and motility factor in human rhabdomyosarcoma tumors"}]},{"@id":"https://cir.nii.ac.jp/crid/1570291224882779008","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Hepatocyte growth factor--scatter factor : mitogen, motogen, and met"}]},{"@id":"https://cir.nii.ac.jp/crid/1570572699954856960","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"cDNA cloning of the human tumor motility-stimulating protein, autotaxin, reveals a homology with phosphodiesterases"}]},{"@id":"https://cir.nii.ac.jp/crid/1571980074838414080","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Tumor autocrine motility 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