紫黒米・黒大豆中の総アントシアニンのｐＨ ｄｉｆｆｅｒｅｎｔｉａｌ法による定量とその試験所間比較による妥当性確認
書誌事項
- タイトル別名
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- Validation of pH Differential Method for the Determination of Total Anthocyanin Content in Black Rice and Black Soybean with Interlaboratory Comparison
- ムラサキ クロゴメ クロ ダイズ チュウ ノ ソウ アントシアニン ノ pH differentialホウ ニ ヨル テイリョウ ト ソノ シケンジョ カン ヒカク ニ ヨル ダトウセイ カクニン
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説明
An interlaboratory study was performed to find a method for the determination of total anthocyanin content in black rice and black soybean, based on the pH differential method. The extraction procedure of anthocyanin in milled materials of black rice and black soybean was optimized ; the recoveries determined for milled materials of rice and soybean spiked with cyanidin-3-glucoside were 98.9% and 92.1%, respectively. Duplicate determinations of total anthocyanin content performed by two technicians on five different test materials on three different days resulted in relative standard deviations of 1.6 to 2.7%. Seven collaborators in six laboratories participated, and measured the total anthocyanin content in five test materials as blind duplicates by the proposed method. A statistical treatment revealed that the relative standard deviation of the repeatability (RSDr) and the relative standard deviation of the reproducibility (RSDR) for the determination of total anthocyanin content were 0.9% to 4.0% and 1.5% to 6.1%, respectively. The HorRat values (RSDR/predicted RSDR) were 0.25 to 1.24. The present study demonstrated that the proposed method for the determination of total anthocyanin content in black rice and black soybean showed good interlaboratory reproducibility in the range from 0.50 to 2.71 mg/g. The expanded uncertainty, obtained by multiplying the standard deviation of the reproducibility (sR) by the coverage factor (k = 2), ranged from 0.021 to 0.326 mg/g.
収録刊行物
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- 分析化学
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分析化学 60 (10), 819-824, 2011
公益社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679638038016
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- NII論文ID
- 10029482396
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- NII書誌ID
- AN00222633
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- COI
- 1:CAS:528:DC%2BC3MXhsValtrfN
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- NDL書誌ID
- 11273458
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- ISSN
- 05251931
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDLサーチ
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- 使用不可