{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1390282679639109632.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.2116/bunsekikagaku.66.89"}},{"identifier":{"@type":"NDL_BIB_ID","@value":"027975687"}},{"identifier":{"@type":"URI","@value":"http://id.ndl.go.jp/bib/027975687"}},{"identifier":{"@type":"URI","@value":"https://ndlsearch.ndl.go.jp/books/R000000004-I027975687"}},{"identifier":{"@type":"URI","@value":"https://www.jstage.jst.go.jp/article/bunsekikagaku/66/2/66_89/_pdf"}},{"identifier":{"@type":"NAID","@value":"130005465010"}}],"dc:title":[{"@language":"ja","@value":"PTFEチューブを分離場とするアミロイド線維の分離分析"},{"@language":"en","@value":"Separation Analysis of Amyloid Fibrils Utilizing a PTFE Tube as a Separation Field"},{"@language":"ja-Kana","@value":"PTFE チューブ オ ブンリジョウ ト スル アミロイド センイ ノ ブンリ ブンセキ"}],"dc:language":"ja","description":[{"type":"abstract","notation":[{"@language":"en","@value":"We developed an easy-to-use analytical method for amyloid fibrils by high-performance liquid chromatography (HPLC) with post column fluorescent labeling.    Thioflavin T (Th T), which was used widly as a labeling agent for amyloid fibrils, is a traditional probe for the confirmation of amyloid fibril formation.    Although the traditional fluorescence spectroscopy measurements are used for monitoring the aggregation reaction, they have no applicability to separation analysis of the amyloid fibrils.    In order to understand the fibril formation process, the development of an efficient method that enables to separate and detect the amyloid fibrils is required.    In this work, Th T labeling of amyloid fibrils in 1 mM hen egg white lysozyme (HEWL) solution was performed following separation of different-sized amyloid fibrils in a PTFE tube (5000 × 0.50 mm i.d.) as a separation field.    The fluorescence was monitored at 485 nm, with the maximum excitation wavelength at 435 nm.    The novel method's sensitivity for the detection of amyloid fibrils was high and compared well with the traditional fluorescence spectroscopy measurements.    In addition, the developed method detected more than 5 peaks within 20 min under the optimum separation conditions.    These results suggest that this analytical method is used to understand the amyloid fibril formation process."}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1420845751135394048","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"00552358"},{"@type":"NRID","@value":"1000000552358"},{"@type":"NRID","@value":"9000018183052"},{"@type":"NRID","@value":"9000404343504"},{"@type":"NRID","@value":"9000292182789"},{"@type":"NRID","@value":"9000399515979"},{"@type":"NRID","@value":"9000398275079"},{"@type":"NRID","@value":"9000347129149"},{"@type":"NRID","@value":"9000408637778"},{"@type":"NRID","@value":"9000410322371"},{"@type":"NRID","@value":"9000391971150"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0145417"}],"foaf:name":[{"@language":"ja","@value":"朝本 紘充"},{"@language":"en","@value":"ASAMOTO Hiromichi"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Liberal Arts and Basic Science, College of Industrial Technology, Nihon University"},{"@language":"ja","@value":"日本大学生産工学部教養・基礎科学系"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282679639109634","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000347129150"}],"foaf:name":[{"@language":"ja","@value":"長嶋 恭介"},{"@language":"en","@value":"NAGASHIMA Kyosuke"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Applied Molecular Chemistry, College of Industrial Technology, Nihon University"},{"@language":"ja","@value":"日本大学生産工学部応用分子化学科"}]},{"@id":"https://cir.nii.ac.jp/crid/1420564276174784896","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"50244421"},{"@type":"NRID","@value":"1000050244421"},{"@type":"NRID","@value":"9000283836922"},{"@type":"NRID","@value":"9000253348768"},{"@type":"NRID","@value":"9000018183055"},{"@type":"NRID","@value":"9000404343502"},{"@type":"NRID","@value":"9000402249123"},{"@type":"NRID","@value":"9000010648806"},{"@type":"NRID","@value":"9000380985522"},{"@type":"NRID","@value":"9000256338400"},{"@type":"NRID","@value":"9000024997674"},{"@type":"NRID","@value":"9000000490531"},{"@type":"NRID","@value":"9000000258535"},{"@type":"NRID","@value":"9000391520130"},{"@type":"NRID","@value":"9000403168578"},{"@type":"NRID","@value":"9000402249101"},{"@type":"NRID","@value":"9000398275077"},{"@type":"NRID","@value":"9000021103460"},{"@type":"NRID","@value":"9000006390718"},{"@type":"NRID","@value":"9000347129151"},{"@type":"NRID","@value":"9000021064416"},{"@type":"NRID","@value":"9000265252218"},{"@type":"NRID","@value":"9000257791712"},{"@type":"NRID","@value":"9000367429053"},{"@type":"NRID","@value":"9000286626247"},{"@type":"NRID","@value":"9000243887240"},{"@type":"NRID","@value":"9000414103679"},{"@type":"NRID","@value":"9000240063152"},{"@type":"NRID","@value":"9000256410534"},{"@type":"NRID","@value":"9000021896855"},{"@type":"NRID","@value":"9000408585688"},{"@type":"NRID","@value":"9000318583273"},{"@type":"NRID","@value":"9000345349261"},{"@type":"NRID","@value":"9000399228458"},{"@type":"NRID","@value":"9000257793125"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0140493"}],"foaf:name":[{"@language":"ja","@value":"中釜 達朗"},{"@language":"en","@value":"NAKAGAMA Tatsuro"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Applied Molecular Chemistry, College of Industrial Technology, Nihon University"},{"@language":"ja","@value":"日本大学生産工学部応用分子化学科"}]},{"@id":"https://cir.nii.ac.jp/crid/1420845751145149440","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"90409087"},{"@type":"NRID","@value":"1000090409087"},{"@type":"NRID","@value":"9000004506586"},{"@type":"NRID","@value":"9000256026183"},{"@type":"NRID","@value":"9000018183054"},{"@type":"NRID","@value":"9000024997675"},{"@type":"NRID","@value":"9000010317797"},{"@type":"NRID","@value":"9000010171159"},{"@type":"NRID","@value":"9000381465523"},{"@type":"NRID","@value":"9000001761152"},{"@type":"NRID","@value":"9000261668379"},{"@type":"NRID","@value":"9000381465585"},{"@type":"NRID","@value":"9000347129152"},{"@type":"NRID","@value":"9000381465487"},{"@type":"NRID","@value":"9000367429054"},{"@type":"NRID","@value":"9000345248383"},{"@type":"NRID","@value":"9000257792854"},{"@type":"NRID","@value":"9000001283699"},{"@type":"NRID","@value":"9000304615439"},{"@type":"NRID","@value":"9000002106201"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0101755"}],"foaf:name":[{"@language":"ja","@value":"齊藤 和憲"},{"@language":"en","@value":"SAITOH Kazunori"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Applied Molecular Chemistry, College of Industrial Technology, Nihon University"},{"@language":"ja","@value":"日本大学生産工学部応用分子化学科"}]},{"@id":"https://cir.nii.ac.jp/crid/1030848083272630401","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"70200087"},{"@type":"NRID","@value":"1000070200087"},{"@type":"NRID","@value":"9000253468152"},{"@type":"NRID","@value":"9000253468628"},{"@type":"NRID","@value":"9000254061264"},{"@type":"NRID","@value":"9000253155532"},{"@type":"NRID","@value":"9000254616895"},{"@type":"NRID","@value":"9000347129153"},{"@type":"NRID","@value":"9000254061380"}],"foaf:name":[{"@language":"ja","@value":"南澤 宏明"},{"@language":"en","@value":"MINAMISAWA Hiroaki"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Liberal Arts and Basic Science, College of Industrial Technology, Nihon University"},{"@language":"ja","@value":"日本大学生産工学部教養・基礎科学系"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"05251931"},{"@type":"NDL_BIB_ID","@value":"000000021279"},{"@type":"ISSN","@value":"05251931"},{"@type":"LISSN","@value":"05251931"},{"@type":"NCID","@value":"AN00222633"}],"prism:publicationName":[{"@language":"ja","@value":"分析化学"},{"@language":"en","@value":"BUNSEKI KAGAKU"},{"@language":"en","@value":"BUNSEKI KAGAKU"},{"@language":"en","@value":"Bunseki kagaku"},{"@language":"ja","@value":"分析化学"}],"dc:publisher":[{"@language":"en","@value":"The Japan Society for Analytical Chemistry"},{"@language":"ja","@value":"公益社団法人 日本分析化学会"}],"prism:publicationDate":"2017","prism:volume":"66","prism:number":"2","prism:startingPage":"89","prism:endingPage":"94"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","url":[{"@id":"http://id.ndl.go.jp/bib/027975687"},{"@id":"https://ndlsearch.ndl.go.jp/books/R000000004-I027975687"},{"@id":"https://www.jstage.jst.go.jp/article/bunsekikagaku/66/2/66_89/_pdf"}],"availableAt":"2017","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=amyloid%20fibril","dc:title":"amyloid fibril"},{"@id":"https://cir.nii.ac.jp/all?q=HPLC-fluorescence%20detecion","dc:title":"HPLC-fluorescence detecion"},{"@id":"https://cir.nii.ac.jp/all?q=PTFE%20tube","dc:title":"PTFE tube"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360011145296745856","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Mechanism of thioflavin T binding to amyloid 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