{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1390282679673259648.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1247/csf.24.111"}},{"identifier":{"@type":"COI","@value":"1:CAS:528:DyaK1MXlsFKhu7g%3D"}},{"identifier":{"@type":"PMID","@value":"10462172"}},{"identifier":{"@type":"NDL_BIB_ID","@value":"4821027"}},{"identifier":{"@type":"URI","@value":"http://id.ndl.go.jp/bib/4821027"}},{"identifier":{"@type":"URI","@value":"https://ndlsearch.ndl.go.jp/books/R000000004-I4821027"}},{"identifier":{"@type":"URI","@value":"http://www.jstage.jst.go.jp/article/csf/24/3/24_3_111/_pdf"}},{"identifier":{"@type":"NAID","@value":"130004137293"}},{"identifier":{"@type":"NAID","@value":"10002420910"}},{"identifier":{"@type":"URI","@value":"https://search.jamas.or.jp/link/ui/2000025820"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@language":"en","@value":"Retinol Esterification Activity Contributes to Retinol Transport in Stellate Cells."}],"dc:language":"en","description":[{"type":"abstract","notation":[{"@language":"en","@value":"The mechanisms of retinol transport and Accumulation in hepatic stellate cells (HSC) remain to be clucidated. Our previous studies suggested that retinol esterfication activity, particularly lecithin: retinol acyltransferase (LRAT) activity, in liver retinoid metabolism is important to elucidate the relationship between retinol uptake by HSC and the esterification of retinol. In the present study, using a human HSC-like cell line, LI90, we demonstrated that retinol esterification activity of LI90, we demonstrated that retinol esterification activity of LI90 cells is similar to that of primary cultures of rat HSC and higher than that of a human hepatoma cell line. Further, since progesterone or diphos-pho-lauroyl-phosphatidylcholine increased retinol esterification activity of LI90 cells, it is likely that LRAT contributes to retinol esterification in LI90. We examined retinol esterification in LI90 cells and clearance of retinol from culture medium. The percentages of both retinol and esterified retinol in LI90 cells increased in a manner dependent of retinol concentration in medium, whereas that of retinol in medium decreased. The percentages of esterified and unesterified retinol in LI90 cells and of retinol in medium were linearly dependent on the logarithm of the initial concentration of retinol in the medium. These results suggest that retinol esterification activity contributes to retinol uptake by HSC and maintenance of non-toxic retinol levels in plasma."}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1420001326230204544","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"30199749"},{"@type":"NRID","@value":"1000030199749"},{"@type":"NRID","@value":"9000021346372"},{"@type":"NRID","@value":"9000257892065"},{"@type":"NRID","@value":"9000258086094"},{"@type":"NRID","@value":"9000021353872"},{"@type":"NRID","@value":"9000404637866"},{"@type":"NRID","@value":"9000410054290"},{"@type":"NRID","@value":"9000347164042"},{"@type":"NRID","@value":"9000403404985"},{"@type":"NRID","@value":"9000345452890"},{"@type":"NRID","@value":"9000016417556"},{"@type":"NRID","@value":"9000242070737"},{"@type":"NRID","@value":"9000018827631"},{"@type":"NRID","@value":"9000398443449"},{"@type":"NRID","@value":"9000398443505"},{"@type":"NRID","@value":"9000398411643"},{"@type":"NRID","@value":"9000021468532"},{"@type":"NRID","@value":"9000021626523"},{"@type":"NRID","@value":"9000021365834"},{"@type":"NRID","@value":"9000021458916"},{"@type":"NRID","@value":"9000001820229"},{"@type":"NRID","@value":"9000398431535"},{"@type":"NRID","@value":"9000398431561"},{"@type":"NRID","@value":"9000398436656"},{"@type":"NRID","@value":"9000398436654"},{"@type":"NRID","@value":"9000391883486"},{"@type":"NRID","@value":"9000318583676"},{"@type":"NRID","@value":"9000347276542"},{"@type":"NRID","@value":"9000398436947"},{"@type":"NRID","@value":"9000398436931"},{"@type":"NRID","@value":"9000398437373"},{"@type":"NRID","@value":"9000398437385"},{"@type":"NRID","@value":"9000255958616"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0050250"}],"foaf:name":[{"@language":"en","@value":"Matsuura Tomokazu"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Internal Medicine I, Jikei University School of Medicine, Minato-ku, Tokyo 105-8461, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282679673259777","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000255958618"}],"foaf:name":[{"@language":"en","@value":"Nagamori Kazuhiro"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Internal Medicine I, Jikei University School of Medicine, Minato-ku, Tokyo 105-8461, Japan"},{"@language":"en","@value":"Division of Clinical Pathology, Tohoku Welfare Pension Hospital, Miyagino-ku, Sendai 983-8512, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282679673259776","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000255958617"}],"foaf:name":[{"@language":"en","@value":"Hasumura Satoshi"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Internal Medicine I, Jikei University School of Medicine, Minato-ku, Tokyo 105-8461, Japan"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"03867196"},{"@type":"EISSN","@value":"13473700"},{"@type":"NDL_BIB_ID","@value":"000000129749"},{"@type":"ISSN","@value":"03867196"},{"@type":"LISSN","@value":"03867196"},{"@type":"NCID","@value":"AA0060007X"}],"prism:publicationName":[{"@language":"en","@value":"Cell Structure and Function"},{"@language":"en","@value":"Cell Struct. Funct."}],"dc:publisher":[{"@language":"en","@value":"Japan Society for Cell Biology"},{"@language":"ja","@value":"日本細胞生物学会"}],"prism:publicationDate":"1999","prism:volume":"24","prism:number":"3","prism:startingPage":"111","prism:endingPage":"116"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","url":[{"@id":"http://id.ndl.go.jp/bib/4821027"},{"@id":"https://ndlsearch.ndl.go.jp/books/R000000004-I4821027"},{"@id":"http://www.jstage.jst.go.jp/article/csf/24/3/24_3_111/_pdf"},{"@id":"https://search.jamas.or.jp/link/ui/2000025820"}],"availableAt":"1999","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=retinoid","dc:title":"retinoid"},{"@id":"https://cir.nii.ac.jp/all?q=vitamin%20A","dc:title":"vitamin A"},{"@id":"https://cir.nii.ac.jp/all?q=hepatic%20stellate%20cell","dc:title":"hepatic stellate cell"},{"@id":"https://cir.nii.ac.jp/all?q=liver","dc:title":"liver"},{"@id":"https://cir.nii.ac.jp/all?q=human","dc:title":"human"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360292619016736896","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Liver takes up retinol-binding protein from plasma."}]},{"@id":"https://cir.nii.ac.jp/crid/1360292619056326656","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Regulation of Hepatic Lecithin: Retinol Acyltransferase Activity by Retinoic Acid"}]},{"@id":"https://cir.nii.ac.jp/crid/1360292621118023424","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Lecithin:Retinol Acyltransferase and Retinyl Ester Hydrolase Activities Are Differentially Regulated by Retinoids and Have Distinct Distributions between Hepatocyte and Nonparenchymal Cell Fractions of Rat Liver ,"}]},{"@id":"https://cir.nii.ac.jp/crid/1360574095019993088","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Regulation of Vitamin A Transport into Cultured Stellate Cells of Rat Liver: Studies by Anchored Cell Analysis and Sorting System"}]},{"@id":"https://cir.nii.ac.jp/crid/1361699995941667200","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Cellular retinol-binding proteins are determinants of retinol uptake and metabolism in stably transfected Caco-2 cells."}]},{"@id":"https://cir.nii.ac.jp/crid/1361699996282353152","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Esterification of retinol in rat liver. Possible participation by cellular retinol-binding protein and cellular retinol-binding protein II."}]},{"@id":"https://cir.nii.ac.jp/crid/1361981471341181952","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Identification and partial characterization of a retinal pigment epithelial membrane receptor for plasma retinol-binding protein"}]},{"@id":"https://cir.nii.ac.jp/crid/1362262944204014336","@type":"Article","relationType":["references","cites"],"jpcoar:relatedTitle":[{"@value":"DEVELOPMENT OF VITAMIN A-RICH LIPID DROPLETS IN MULTIVESICULAR BODIES OF RAT LIVER STELLATE CELLS"}]},{"@id":"https://cir.nii.ac.jp/crid/1362544420040865280","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Retinol esterification by mammary gland microsomes from the lactating rat."}]},{"@id":"https://cir.nii.ac.jp/crid/1363107368847706240","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Retinol Transport in Cultured Stellate Cells of Rat Liver: Studies by Light and Electron Microscope Autoradiography"}]},{"@id":"https://cir.nii.ac.jp/crid/1363107369655687040","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Internalization of retinol-binding protein in parenchymal and stellate cells of rat liver."}]},{"@id":"https://cir.nii.ac.jp/crid/1363107369666724864","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"A lecithin: Retinol acyltransferase activity in human and rat liver"}]},{"@id":"https://cir.nii.ac.jp/crid/1363107371311886848","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Retinoid-mediated induction of the fat-storing phenotype in a liver connective tissue cell line (GRX)"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204697259520","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Extension of Long Cellular Processes of Hepatic Stellate Cells Cultured on Extracellular Type I Collagen Gel by Microtubule Assembly: Observation Utilizing Time-lapse Video-microscopy."}]},{"@id":"https://cir.nii.ac.jp/crid/1390001205701034240","@type":"Article","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@language":"ja","@value":"ビタミンA貯蔵細胞研究からの新知見"},{"@language":"en","@value":"Frontiers of Vitamin A-storing Cell Research"},{"@language":"ja-Kana","@value":"ビタミン A チョゾウ サイボウ ケンキュウ カラ ノ シン チケン"}]},{"@id":"https://cir.nii.ac.jp/crid/1521699229883476864","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@value":"Extension of Long Cellular Processes of Hepatic Stellate Cells Cultured on Extracellular Type 1 Collagen Gel by Microtubule Assembly:Observation Utilizing Time-lapse Video-microscopy"},{"@language":"ja-Kana","@value":"Extension of Long Cellular Processes of"}]},{"@id":"https://cir.nii.ac.jp/crid/1570291226738473856","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Retinol transport in cultured stellate cells of rat liver : studies by Light and electron microscope autoradiography."}]},{"@id":"https://cir.nii.ac.jp/crid/1570572699143239296","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Retinol transport in cultured fat-storing cells of rat liver. 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