{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1390282679691059328.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.5358/hsj.34.177"}},{"identifier":{"@type":"NDL_BIB_ID","@value":"026695792"}},{"identifier":{"@type":"URI","@value":"http://id.ndl.go.jp/bib/026695792"}},{"identifier":{"@type":"URI","@value":"https://ndlsearch.ndl.go.jp/books/R000000004-I026695792"}},{"identifier":{"@type":"NAID","@value":"130005095175"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@language":"en","@value":"Development and Characterization of 12 Microsatellite Markers for the Hokkaido Salamander (<i>Hynobius retardatus</i>)"},{"@value":"Development and Characterization of 12 Microsatellite Markers for the Hokkaido Salamander (Hynobius retardatus)"},{"@value":"Development and characterization of 12 microsatellite markers for the Hokkaido salamander (<italic>Hynobius retardatus</italic>)"}],"dc:language":"en","description":[{"type":"abstract","notation":[{"@language":"en","@value":"The Hokkaido salamander, <i>Hynobius retardatus</i> (Caudata: Hynobiidae), is a common salamander species distributed throughout Hokkaido Island, Japan. Because of its regional morphological diversity and flexible phenotypic plasticity, its demographic history and the causes of its distribution patterns are of intrinsic interest to evolutionary biologists. To address these issues, a microsatellite marker is an ideal genetic marker for inferring gene flows and hybridizations between populations on a fine scale. We therefore utilized an Ion PGM™ sequencing system to screen the <i>H. retardatus</i> genome for microsatellite markers. As the result, we identified 12 polymorphic markers. We then tested the usefulness of these markers in <i>H. retardatus</i> by genotyping 20 individuals from each of two regional populations (the Erimo and Nopporo populations). The total number of alleles ranged from 3 to 8, and the expected heterozygosities per locus ranged from 0.049 to 0.803. We expect the developed markers to be useful for future studies of demography of this species."}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1420564276187594240","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"60632635"},{"@type":"NRID","@value":"1000060632635"},{"@type":"CINII_AUTHOR_ID","@value":"DB00400469"},{"@type":"URI","@value":"https://ci.nii.ac.jp/author/DB00400469#entity"},{"@type":"URI","@value":"https://viaf.org/viaf/NII%7CDB00400469"},{"@type":"NRID","@value":"9000399787704"},{"@type":"NRID","@value":"9000414491116"},{"@type":"NRID","@value":"9000398150763"},{"@type":"NRID","@value":"9000335564548"},{"@type":"NRID","@value":"9000360565507"},{"@type":"NRID","@value":"9000303161428"},{"@type":"NRID","@value":"9000411229143"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/mmatsunami"}],"foaf:name":[{"@language":"en","@value":"MATSUNAMI Masatoshi"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Graduate School of Fisheries Sciences, Hokkaido University"},{"@language":"en","@value":"Laboratory of Ecology and Genetics, Graduate School of Environmental Science, Hokkaido University"}]},{"@id":"https://cir.nii.ac.jp/crid/1420564276158629120","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"00507197"},{"@type":"NRID","@value":"1000000507197"},{"@type":"NRID","@value":"9000254230872"},{"@type":"NRID","@value":"9000415151374"},{"@type":"NRID","@value":"9000402776881"},{"@type":"NRID","@value":"9000402776885"},{"@type":"NRID","@value":"9000327348362"},{"@type":"NRID","@value":"9000392827894"},{"@type":"NRID","@value":"9000335564549"},{"@type":"NRID","@value":"9000345382842"},{"@type":"NRID","@value":"9000272988301"},{"@type":"NRID","@value":"9000267790507"},{"@type":"NRID","@value":"9000400183730"},{"@type":"NRID","@value":"9000005183443"},{"@type":"NRID","@value":"9000408427129"},{"@type":"NRID","@value":"9000386285693"},{"@type":"NRID","@value":"9000347077129"},{"@type":"NRID","@value":"9000303161429"},{"@type":"NRID","@value":"9000255960852"},{"@type":"NRID","@value":"9000283457496"},{"@type":"NRID","@value":"9000398293374"},{"@type":"NRID","@value":"9000413945671"},{"@type":"NRID","@value":"9000398293363"},{"@type":"NRID","@value":"9000403401522"},{"@type":"NRID","@value":"9000267783204"},{"@type":"NRID","@value":"9000019097751"},{"@type":"NRID","@value":"9000400183598"},{"@type":"NRID","@value":"9000254701834"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/igatake"}],"foaf:name":[{"@language":"en","@value":"IGAWA Takeshi"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Graduate School of International Development and Cooperation, Hiroshima University"}]},{"@id":"https://cir.nii.ac.jp/crid/1420845751163159680","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"50623534"},{"@type":"NRID","@value":"1000050623534"},{"@type":"NRID","@value":"9000413483725"},{"@type":"NRID","@value":"9000413475118"},{"@type":"NRID","@value":"9000364752367"},{"@type":"NRID","@value":"9000345382843"},{"@type":"NRID","@value":"9000272988302"},{"@type":"NRID","@value":"9000303161430"},{"@type":"NRID","@value":"9000255960853"},{"@type":"NRID","@value":"9000015319853"},{"@type":"NRID","@value":"9000007054112"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/masafumi-nozawa"}],"foaf:name":[{"@language":"en","@value":"NOZAWA Masafumi"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Center for Information Biology, National Institute of Genetics"},{"@language":"en","@value":"Department of Genetics, The Graduate University for Advanced Studies (SOKENDAI)"}]},{"@id":"https://cir.nii.ac.jp/crid/1420001326223982208","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"70447069"},{"@type":"NRID","@value":"1000070447069"},{"@type":"NRID","@value":"9000409978272"},{"@type":"NRID","@value":"9000237783873"},{"@type":"NRID","@value":"9000237783824"},{"@type":"NRID","@value":"9000335564550"},{"@type":"NRID","@value":"9000006401186"},{"@type":"NRID","@value":"9000237783878"},{"@type":"NRID","@value":"9000237783886"},{"@type":"NRID","@value":"9000237783850"},{"@type":"NRID","@value":"9000237782830"},{"@type":"NRID","@value":"9000392032174"},{"@type":"NRID","@value":"9000415174201"},{"@type":"NRID","@value":"9000303161431"},{"@type":"NRID","@value":"9000237783879"},{"@type":"NRID","@value":"9000242347860"},{"@type":"NRID","@value":"9000403990329"},{"@type":"NRID","@value":"9000392031575"},{"@type":"NRID","@value":"9000415155622"},{"@type":"NRID","@value":"9000411229146"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0074111"}],"foaf:name":[{"@language":"en","@value":"MICHIMAE Hirofumi"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Division of Biostatics, School of Pharmaceutical Sciences, Kitasato University"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282679691059328","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000303161432"}],"foaf:name":[{"@language":"en","@value":"MIURA Toru"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Laboratory of Ecology and Genetics, Graduate School of Environmental Science, Hokkaido University"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282679691059331","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000303161433"}],"foaf:name":[{"@language":"en","@value":"NISHIMURA Kinya"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Graduate School of Fisheries Sciences, Hokkaido University"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"13455834"},{"@type":"EISSN","@value":"18811019"},{"@type":"NDL_BIB_ID","@value":"000000161447"},{"@type":"ISSN","@value":"13455834"},{"@type":"LISSN","@value":"13455834"},{"@type":"NCID","@value":"AA12246863"}],"prism:publicationName":[{"@language":"en","@value":"Current Herpetology"},{"@language":"ja","@value":"Ｃｕｒｒｅｎｔ　ｈｅｒｐｅｔｏｌｏｇｙ"},{"@language":"en","@value":"Curr. Herpetol."},{"@language":"en","@value":"Current Herpetol."},{"@language":"ja","@value":"Ｃｕｒｒｅｎｔ　Ｈｅｒｐｅｔｏｌｏｇｙ"},{"@language":"en","@value":"Curr.Herpetol."},{"@language":"ja","@value":"Ｃｕｒｒ．Ｈｅｒｐｅｔｏｌ．"},{"@language":"en","@value":"Current herpetology"}],"dc:publisher":[{"@language":"en","@value":"The Herpetological Society of Japan"},{"@language":"ja","@value":"日本爬虫両棲類学会"}],"prism:publicationDate":"2015","prism:volume":"34","prism:number":"2","prism:startingPage":"177","prism:endingPage":"181"},"reviewed":"false","url":[{"@id":"http://id.ndl.go.jp/bib/026695792"},{"@id":"https://ndlsearch.ndl.go.jp/books/R000000004-I026695792"}],"availableAt":"2015","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Urodela","dc:title":"Urodela"},{"@id":"https://cir.nii.ac.jp/all?q=Salamander","dc:title":"Salamander"},{"@id":"https://cir.nii.ac.jp/all?q=Genetic%20diversity","dc:title":"Genetic diversity"},{"@id":"https://cir.nii.ac.jp/all?q=Population%20genetics","dc:title":"Population genetics"},{"@id":"https://cir.nii.ac.jp/all?q=Microsatellite%20markers","dc:title":"Microsatellite markers"}],"project":[{"@id":"https://cir.nii.ac.jp/crid/1040000782317512960","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"26830144"},{"@type":"JGN","@value":"JP26830144"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26830144/"}],"notation":[{"@language":"ja","@value":"免疫関連遺伝子に基づく絶滅危惧両生類の疫学的リスク予測とツボカビ耐性機構の検証"},{"@language":"en","@value":"Epidemiological risk prediction and validation of fungal resistance mechanism of threatened amphibian species based on immunity related genes"}]}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050025031482596736","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Maternal effects on phenotypic plasticity in larvae of the salamander Hynobius retardatus"},{"@value":"Maternal effects on phenotypic plasticity in larvae of the salamander <italic>Hynobius retardatus</italic>"}]},{"@id":"https://cir.nii.ac.jp/crid/1050306506454771328","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Population Structure and Evolution after Speciation of the Hokkaido Salamander (Hynobius retardatus)"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004238332779136","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Population Structure of the Salamander Hynobius retardatus Inferred from a Partial Sequence of the Mitochondrial DNA Control Region"}]},{"@id":"https://cir.nii.ac.jp/crid/1360574094126076288","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"P450 aromatase expression in the temperature-sensitive sexual differentiation of salamander (Hynobius retardatus) gonads"}]},{"@id":"https://cir.nii.ac.jp/crid/1360855567865599104","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Microsatellites: simple sequences with complex evolution"}]},{"@id":"https://cir.nii.ac.jp/crid/1361981470489081984","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"SolexaQA: At-a-glance quality assessment of Illumina second-generation sequencing data"}]},{"@id":"https://cir.nii.ac.jp/crid/1362825894711839360","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"GENEPOP (Version 1.2): Population Genetics Software for Exact Tests and Ecumenicism"}]},{"@id":"https://cir.nii.ac.jp/crid/1362825895768190464","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"<scp>msatcommander</scp>: detection of microsatellite repeat arrays and automated, locus‐specific primer design"}]},{"@id":"https://cir.nii.ac.jp/crid/1363107368483176192","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"A post-labeling method for multiplexed and multicolored genotyping analysis of SSR, indel and SNP markers in single tube with bar-coded split tag (BStag)"}]},{"@id":"https://cir.nii.ac.jp/crid/1363388844124984320","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"<scp>genalex</scp> 6: genetic analysis in Excel. 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