Development and Characterization of 12 Microsatellite Markers for the Hokkaido Salamander (<i>Hynobius retardatus</i>)

DOI Web Site 3 Citations 12 References
  • MATSUNAMI Masatoshi
    Graduate School of Fisheries Sciences, Hokkaido University Laboratory of Ecology and Genetics, Graduate School of Environmental Science, Hokkaido University
  • IGAWA Takeshi
    Graduate School of International Development and Cooperation, Hiroshima University
  • NOZAWA Masafumi
    Center for Information Biology, National Institute of Genetics Department of Genetics, The Graduate University for Advanced Studies (SOKENDAI)
  • MICHIMAE Hirofumi
    Division of Biostatics, School of Pharmaceutical Sciences, Kitasato University
  • MIURA Toru
    Laboratory of Ecology and Genetics, Graduate School of Environmental Science, Hokkaido University
  • NISHIMURA Kinya
    Graduate School of Fisheries Sciences, Hokkaido University

Bibliographic Information

Other Title
  • Development and Characterization of 12 Microsatellite Markers for the Hokkaido Salamander (Hynobius retardatus)
  • Development and characterization of 12 microsatellite markers for the Hokkaido salamander (<italic>Hynobius retardatus</italic>)

Search this article

Description

The Hokkaido salamander, Hynobius retardatus (Caudata: Hynobiidae), is a common salamander species distributed throughout Hokkaido Island, Japan. Because of its regional morphological diversity and flexible phenotypic plasticity, its demographic history and the causes of its distribution patterns are of intrinsic interest to evolutionary biologists. To address these issues, a microsatellite marker is an ideal genetic marker for inferring gene flows and hybridizations between populations on a fine scale. We therefore utilized an Ion PGM™ sequencing system to screen the H. retardatus genome for microsatellite markers. As the result, we identified 12 polymorphic markers. We then tested the usefulness of these markers in H. retardatus by genotyping 20 individuals from each of two regional populations (the Erimo and Nopporo populations). The total number of alleles ranged from 3 to 8, and the expected heterozygosities per locus ranged from 0.049 to 0.803. We expect the developed markers to be useful for future studies of demography of this species.

Journal

  • Current Herpetology

    Current Herpetology 34 (2), 177-181, 2015

    The Herpetological Society of Japan

Citations (3)*help

See more

References(12)*help

See more

Related Projects

See more

Keywords

Details 詳細情報について

Report a problem

Back to top