Development and Characterization of 12 Microsatellite Markers for the Hokkaido Salamander (Hynobius retardatus)
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- MATSUNAMI Masatoshi
- Graduate School of Fisheries Sciences, Hokkaido University Laboratory of Ecology and Genetics, Graduate School of Environmental Science, Hokkaido University
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- IGAWA Takeshi
- Graduate School of International Development and Cooperation, Hiroshima University
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- NOZAWA Masafumi
- Center for Information Biology, National Institute of Genetics Department of Genetics, The Graduate University for Advanced Studies (SOKENDAI)
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- MICHIMAE Hirofumi
- Division of Biostatics, School of Pharmaceutical Sciences, Kitasato University
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- MIURA Toru
- Laboratory of Ecology and Genetics, Graduate School of Environmental Science, Hokkaido University
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- NISHIMURA Kinya
- Graduate School of Fisheries Sciences, Hokkaido University
書誌事項
- タイトル別名
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- Development and Characterization of 12 Microsatellite Markers for the Hokkaido Salamander (<i>Hynobius retardatus</i>)
- Development and characterization of 12 microsatellite markers for the Hokkaido salamander (<italic>Hynobius retardatus</italic>)
- 公開日
- 2015
- 資源種別
- journal article
- DOI
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- 10.5358/hsj.34.177
- 公開者
- 日本爬虫両棲類学会
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説明
The Hokkaido salamander, Hynobius retardatus (Caudata: Hynobiidae), is a common salamander species distributed throughout Hokkaido Island, Japan. Because of its regional morphological diversity and flexible phenotypic plasticity, its demographic history and the causes of its distribution patterns are of intrinsic interest to evolutionary biologists. To address these issues, a microsatellite marker is an ideal genetic marker for inferring gene flows and hybridizations between populations on a fine scale. We therefore utilized an Ion PGM™ sequencing system to screen the H. retardatus genome for microsatellite markers. As the result, we identified 12 polymorphic markers. We then tested the usefulness of these markers in H. retardatus by genotyping 20 individuals from each of two regional populations (the Erimo and Nopporo populations). The total number of alleles ranged from 3 to 8, and the expected heterozygosities per locus ranged from 0.049 to 0.803. We expect the developed markers to be useful for future studies of demography of this species.
収録刊行物
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- Ｃｕｒｒｅｎｔ ｈｅｒｐｅｔｏｌｏｇｙ
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Ｃｕｒｒｅｎｔ ｈｅｒｐｅｔｏｌｏｇｙ 34 (2), 177-181, 2015
日本爬虫両棲類学会
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詳細情報 詳細情報について
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- CRID
- 1390282679691059328
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- NII論文ID
- 130005095175
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- NII書誌ID
- AA12246863
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- ISSN
- 18811019
- 13455834
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- NDL書誌ID
- 026695792
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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- JaLC
- NDLサーチ
- Crossref
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- 使用不可
