{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1390282679697275136.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1270/jsbbs1951.44.333"}},{"identifier":{"@type":"NDL_BIB_ID","@value":"3895913"}},{"identifier":{"@type":"URI","@value":"http://id.ndl.go.jp/bib/3895913"}},{"identifier":{"@type":"URI","@value":"https://ndlsearch.ndl.go.jp/books/R000000004-I3895913"}},{"identifier":{"@type":"URI","@value":"http://www.jstage.jst.go.jp/article/jsbbs1951/44/3/44_3_333/_pdf"}},{"identifier":{"@type":"NAID","@value":"130003479117"}},{"identifier":{"@type":"NAID","@value":"110001807782"}}],"dc:title":[{"@language":"en","@value":"Simple Method for Overcoming the Lethality Observed in the Hybrid between Nicotiana suaveolens and N. tabacum."},{"@language":"ja","@value":"タバコ属種間雑種(Nicotiana suaveolens×N. tabacum)に現れる致死性を克服するための簡便な方法"},{"@language":"ja-Kana","@value":"タバコゾクシュカン ザッシュ Nicotiana suaveolens N.t"}],"dc:language":"en","description":[{"type":"abstract","notation":[{"@language":"en","@value":"Hybrid plants between N. suaveolens and N. tabacum plants exhibit lethality under standard culture conditions and die at the cotyledonary stage. For overcoming this type of high brid lethality, the effect of cytokinin was tested. The intact hybrid seedlings were cultured on media containing cytokinin (0-10mg/l kinetin or 6-benzylaminopurine (BAP)).Adventitious buds were regeneratcd from the base of the hypocotyl or the primary root of the dying seedlings. The regenerated shoots were transferred to the medium containing 2mg/l 3-indoleacetic acid (IAA) to accelerate rooting. The hybrid seedlings maintained a vigorous growth without developing any lethal symptoms up to maturity in a greenhouse. Forty chromosomes were counted in their somatic cells and their morphological characters were intermediate between those of parental species. Intact seedling culture with the addition of cytokinin was found to be a very simple method for overcoming lethality in this cross-combination."},{"@value":"Nicotiana層内の種間雑種，N. suaveolens×N. tabacum (♂)は成育初期に雑種致死する。雑種致死の克服を目的として，播種後4日目の雑種実生をO-10mg/lのカイネチンまたはBAPを添加した1/2MS培地に移植し，温度条件が28℃，終日照明のインキュベーター内で培養した。培養を開始して数日後に，本組み合わせ雑種致死の特徴である胚軸の褐変が始まり，約1週間後には全体が褐変した。培地にサイトカイニンを添加しなかった処理区では，そのまますべての雑種実生が枯死した(Fig.1A)。ところが，培地にサイトカイニンを添加したものでは，褐変した実生の根および胚軸の基部に緑色の不定芽が現れ，培養を開始してから6週間後には多数のシュートが形成された(Fig. 2)。サイトカイニンとしてカイネチンを添加したものでは2および1Omg/l添加区で，BAPを添加したものでは2mg/l添加区でマルチプルシュートが観察された。カイネチン，BAPともに0.2mg/l添加区で雑種致死の症状を示さない健全なシュートが得られた(Table 1)。このように雑種致死実生からの致死を示さない不定芽の直接的な分化には，培地に植物成長調節物質としてサイトカイニンを単独で添加することが有効であると示唆された。雑種致死実生から再分化したシュートを2mg/lのオーキシンを含む培地に移植し発根を促したところ，ほぼすべての個体が発根した。これらの植物体は，檸種致死の症状をまったく示さず温室で順調に成育しつづけ開花した。開花植物の形態はほぼ両親の中間であり，花色は淡い桃色で両親の中間色だった(Fig 3)。根端の染色体数は40本であり，両親のゲノムの和であった。これらの結果より，本法によって得られた植物体は完全な雑種植物であることが証明された。以上，本交雑組み合わせにおいて従来よりも簡便に雑種致死を克服する方法を確立した。今後，雑種致死がみられる他の交雑組み合わせについて，本法の適用が期待される。"}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1420001326214774400","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"90292482"},{"@type":"NRID","@value":"1000090292482"},{"@type":"NRID","@value":"9000020297182"},{"@type":"NRID","@value":"9000002623722"},{"@type":"NRID","@value":"9000253115544"},{"@type":"NRID","@value":"9000392685573"},{"@type":"NRID","@value":"9000254140927"},{"@type":"NRID","@value":"9000283460584"},{"@type":"NRID","@value":"9000003118135"},{"@type":"NRID","@value":"9000020468344"},{"@type":"NRID","@value":"9000392689150"},{"@type":"NRID","@value":"9000253114796"},{"@type":"NRID","@value":"9000003778902"},{"@type":"NRID","@value":"9000392681643"},{"@type":"NRID","@value":"9000392682274"},{"@type":"NRID","@value":"9000020543606"},{"@type":"NRID","@value":"9000021839105"},{"@type":"NRID","@value":"9000021874143"},{"@type":"NRID","@value":"9000020499213"},{"@type":"NRID","@value":"9000021490082"},{"@type":"NRID","@value":"9000020148661"},{"@type":"NRID","@value":"9000304616486"},{"@type":"NRID","@value":"9000252947232"},{"@type":"NRID","@value":"9000254567804"},{"@type":"NRID","@value":"9000401863864"},{"@type":"NRID","@value":"9000005991449"},{"@type":"NRID","@value":"9000252943694"},{"@type":"NRID","@value":"9000020219924"},{"@type":"NRID","@value":"9000020398428"},{"@type":"NRID","@value":"9000020397879"},{"@type":"NRID","@value":"9000252950624"},{"@type":"NRID","@value":"9000252938281"},{"@type":"NRID","@value":"9000020446062"},{"@type":"NRID","@value":"9000020376173"},{"@type":"NRID","@value":"9000254140928"},{"@type":"NRID","@value":"9000003246293"},{"@type":"NRID","@value":"9000021875632"},{"@type":"NRID","@value":"9000021779984"},{"@type":"NRID","@value":"9000003342284"},{"@type":"NRID","@value":"9000003246292"},{"@type":"NRID","@value":"9000254264419"},{"@type":"NRID","@value":"9000401885454"},{"@type":"NRID","@value":"9000020380430"},{"@type":"NRID","@value":"9000252949456"},{"@type":"NRID","@value":"9000401575475"},{"@type":"NRID","@value":"9000392692931"},{"@type":"NRID","@value":"9000020489540"},{"@type":"NRID","@value":"9000021778816"},{"@type":"NRID","@value":"9000254367820"},{"@type":"NRID","@value":"9000253114403"},{"@type":"NRID","@value":"9000401590437"},{"@type":"NRID","@value":"9000392685883"},{"@type":"NRID","@value":"9000021873654"},{"@type":"NRID","@value":"9000414834664"},{"@type":"NRID","@value":"9000401588607"},{"@type":"NRID","@value":"9000401885453"},{"@type":"NRID","@value":"9000399811347"},{"@type":"NRID","@value":"9000256383173"},{"@type":"NRID","@value":"9000020493420"},{"@type":"NRID","@value":"9000255999487"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0110629"}],"foaf:name":[{"@language":"en","@value":"Inoue Eiichi"},{"@language":"ja","@value":"井上 栄一"}]},{"@id":"https://cir.nii.ac.jp/crid/1030003658450064768","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"00181826"},{"@type":"NRID","@value":"1000000181826"},{"@type":"NRID","@value":"9000003091944"},{"@type":"NRID","@value":"9000003118976"},{"@type":"NRID","@value":"9000256382998"},{"@type":"NRID","@value":"9000265562155"},{"@type":"NRID","@value":"9000003117288"},{"@type":"NRID","@value":"9000351100918"},{"@type":"NRID","@value":"9000252998998"},{"@type":"NRID","@value":"9000003117363"},{"@type":"NRID","@value":"9000253001301"},{"@type":"NRID","@value":"9000018600608"},{"@type":"NRID","@value":"9000261673278"},{"@type":"NRID","@value":"9000003121002"},{"@type":"NRID","@value":"9000391407378"},{"@type":"NRID","@value":"9000253000669"},{"@type":"NRID","@value":"9000007055897"},{"@type":"NRID","@value":"9000256000140"},{"@type":"NRID","@value":"9000255694316"},{"@type":"NRID","@value":"9000020454306"},{"@type":"NRID","@value":"9000252999382"},{"@type":"NRID","@value":"9000003118138"},{"@type":"NRID","@value":"9000255999198"},{"@type":"NRID","@value":"9000006596481"},{"@type":"NRID","@value":"9000255816136"},{"@type":"NRID","@value":"9000283621326"},{"@type":"NRID","@value":"9000015620274"},{"@type":"NRID","@value":"9000253000842"},{"@type":"NRID","@value":"9000256383174"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0060033"}],"foaf:name":[{"@language":"en","@value":"Marubashi Wataru"},{"@language":"ja","@value":"丸橋 亘"}]},{"@id":"https://cir.nii.ac.jp/crid/1410001204424692352","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000003116458"},{"@type":"NRID","@value":"9000253001302"},{"@type":"NRID","@value":"9000253000670"}],"foaf:name":[{"@language":"en","@value":"Niwa Masaru"},{"@language":"ja","@value":"丹羽 勝"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"05363683"},{"@type":"EISSN","@value":"2185291X"},{"@type":"NDL_BIB_ID","@value":"000000001389"},{"@type":"ISSN","@value":"05363683"},{"@type":"LISSN","@value":"05363683"},{"@type":"NCID","@value":"AN10322962"}],"prism:publicationName":[{"@language":"ja","@value":"育種学雑誌"},{"@language":"en","@value":"Japanese Journal of Breeding"},{"@language":"en","@value":"Ikushugaku zasshi"},{"@language":"ja","@value":"育雑"},{"@language":"en","@value":"Jpn.J. Breed."}],"dc:publisher":[{"@language":"en","@value":"Japanese Society of Breeding"},{"@language":"ja","@value":"日本育種学会"}],"prism:publicationDate":"1994","prism:volume":"44","prism:number":"3","prism:startingPage":"333","prism:endingPage":"336"},"reviewed":"false","url":[{"@id":"http://id.ndl.go.jp/bib/3895913"},{"@id":"https://ndlsearch.ndl.go.jp/books/R000000004-I3895913"},{"@id":"http://www.jstage.jst.go.jp/article/jsbbs1951/44/3/44_3_333/_pdf"}],"availableAt":"1994","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=cytokinin","dc:title":"cytokinin"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360584340704841856","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Understanding and overcoming hybrid lethality in seed and seedling stages as barriers to hybridization and gene flow"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204327515392","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Characterization of hybrid seedlings from crosses of <i>Nicotiana stocktonii</i> Brandegee×<i>N. tabacum</i> L. and <i>N. stocktonii</i>×progenitors of <i>N. tabacum</i>"},{"@value":"Characterization of hybrid seedlings from crosses of Nicotiana stocktonii Brandegee×N. tabacum L. and N. stocktonii×progenitors of N. tabacum"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679696511360","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Improvement of the Method for Overcoming the Hybrid Lethality between Nicotiana suaveolens and N. tabacum by Culture of F<SUB>1</SUB> Seeds in Liquid Media Containing Cytokinins"},{"@language":"ja","@value":"サイトカイニンを添加した液体培地でのF<SUB>1</SUB>種子(Nicotiana suaveolens×N.tabacum)の培養による雑種致死克服の効率化"},{"@value":"Improvement of the method for overcoming the hybrid lethality between Nicotiana suaveolens and N.tabacum by culture of F1 seeds in liquid media containing cytokinis"},{"@language":"ja-Kana","@value":"Improvement of the method for overcomin"},{"@value":"Improvement of the method for overcoming the hybrid lethality between Nicotiana suaveolens and N. tabacum by culture of F1 seeds in liquid media containing cytokinins"}]}],"dataSourceIdentifier":[{"@type":"JALC","@value":"oai:japanlinkcenter.org:0002856011"},{"@type":"NDL_SEARCH","@value":"oai:ndlsearch.ndl.go.jp:R000000004-I3895913"},{"@type":"CROSSREF","@value":"10.1270/jsbbs1951.44.333"},{"@type":"CIA","@value":"110001807782"},{"@type":"CIA","@value":"130003479117"},{"@type":"CROSSREF","@value":"10.1270/jsbbs1951.47.211_references_DOI_DuKbopeB28HNeULKUu8JsAfEPiv"},{"@type":"CROSSREF","@value":"10.3389/fpls.2023.1219417_references_DOI_DuKbopeB28HNeULKUu8JsAfEPiv"},{"@type":"CROSSREF","@value":"10.5511/plantbiotechnology.15.0402a_references_DOI_DuKbopeB28HNeULKUu8JsAfEPiv"}]}